ID L0DBI9_SINAD Unreviewed; 686 AA.
AC L0DBI9;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE SubName: Full=Uncharacterized radical SAM protein YgiQ {ECO:0000313|EMBL:AGA26612.1};
GN OrderedLocusNames=Sinac_2294 {ECO:0000313|EMBL:AGA26612.1};
OS Singulisphaera acidiphila (strain ATCC BAA-1392 / DSM 18658 / VKM B-2454 /
OS MOB10).
OC Bacteria; Planctomycetota; Planctomycetia; Isosphaerales; Isosphaeraceae;
OC Singulisphaera.
OX NCBI_TaxID=886293 {ECO:0000313|EMBL:AGA26612.1, ECO:0000313|Proteomes:UP000010798};
RN [1] {ECO:0000313|EMBL:AGA26612.1, ECO:0000313|Proteomes:UP000010798}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1392 / DSM 18658 / VKM B-2454 / MOB10
RC {ECO:0000313|Proteomes:UP000010798};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Chertkov O.,
RA Kyrpides N., Mavromatis K., Ivanova N., Brettin T., Detter J.C., Han C.,
RA Larimer F., Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P.,
RA Woyke T., Wu D., Tindall B., Pomrenke H., Brambilla E., Klenk H.-P.,
RA Eisen J.A.;
RT "Complete sequence of chromosome of Singulisphaera acidiphila DSM 18658.";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01251};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|HAMAP-Rule:MF_01251};
CC -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000256|HAMAP-
CC Rule:MF_01251}.
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DR EMBL; CP003364; AGA26612.1; -; Genomic_DNA.
DR RefSeq; WP_015245768.1; NZ_JH621489.1.
DR AlphaFoldDB; L0DBI9; -.
DR STRING; 886293.Sinac_2294; -.
DR KEGG; saci:Sinac_2294; -.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_018288_2_0_0; -.
DR OrthoDB; 9803479at2; -.
DR Proteomes; UP000010798; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR HAMAP; MF_01251; UPF0313; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR022946; UPF0313.
DR InterPro; IPR024560; UPF0313_C.
DR InterPro; IPR013704; UPF0313_N.
DR NCBIfam; TIGR03904; SAM_YgiQ; 1.
DR PANTHER; PTHR32331; UPF0313 PROTEIN YGIQ; 1.
DR PANTHER; PTHR32331:SF0; UPF0313 PROTEIN YGIQ; 1.
DR Pfam; PF11842; DUF3362; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF08497; Radical_SAM_N; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01069; UPF0313; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|HAMAP-
KW Rule:MF_01251};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01251}; Reference proteome {ECO:0000313|Proteomes:UP000010798};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01251}.
FT DOMAIN 326..597
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 650..686
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 340
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 344
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 347
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
SQ SEQUENCE 686 AA; 76422 MW; 0C0A3627CE0B672C CRC64;
MTTGTVAPPP CSSRNANPNV APTQLPVLPL VPLALPHLPA SRAEMERRGW NEVDVVFVTG
DAYVDHPAFA MGILGRILEA AGFRVAILSQ PDWRSADPWR EFGRPRLFFG ISAGNMDSMI
NHYTANKKVR NDDAYSPGGR IGLRPDRATM PYCQRAREAF PGVPVIAGGV EASLRRLAHY
DYWSDTVKRS ILLDAKADLL VFGMGEQPIV ELARRLAAGE SVRDLRDMRG VAYTLGAKET
PPDDAFVLPT FEEVKTDRHK FAQATRIIHQ ETNPLNAKRL VQYHDRQAVV VNPPALPISQ
DDMDRIYGLP YTRRPHPKYK NEKIPAFETV KDSVTIMRGC FGGCTFCSIT AHQGRIIQSR
SQESVLSEIR KMGEEPSFSG IVSDIGGPTA NMYQMRCTRP EVEAKCKRQS CVHPKICKLL
GTDHGPLVQL MRESREIPGV RKVMVASGVR MDLAQRSPEY MVELAGHHVG GHLKVAPEHT
DPEVLKRMKK PEVNDYEGFA DAFKEASATA GKPKQFLVPY FIASHPGSDL DAMIDLALFL
KRNGYRPDQV QDFIPAPFDV ATCMYYTGLD PFTGEEVYTA RNLRDRKLQR ALLQFFKPEN
YFEVREALQK AGRQELIGSG CDALIPANPP KAALQARMAK ANRALGEGRY VHTIPKPAGE
SEAKQANPQS TGYRPDRKSA KRRPRR
//
