ID   L0JDG1_PREDD            Unreviewed;      1028 AA.
AC   L0JDG1;
DT   06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT   06-MAR-2013, sequence version 1.
DT   27-MAR-2024, entry version 41.
DE   RecName: Full=arabinogalactan endo-beta-1,4-galactanase {ECO:0000256|ARBA:ARBA00012556};
DE            EC=3.2.1.89 {ECO:0000256|ARBA:ARBA00012556};
GN   OrderedLocusNames=Prede_1577 {ECO:0000313|EMBL:AGB28883.1};
OS   Prevotella dentalis (strain ATCC 49559 / DSM 3688 / JCM 13448 / NCTC 12043
OS   / ES 2772) (Mitsuokella dentalis).
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC   Prevotella.
OX   NCBI_TaxID=908937 {ECO:0000313|EMBL:AGB28883.1, ECO:0000313|Proteomes:UP000010862};
RN   [1] {ECO:0000313|Proteomes:UP000010862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49559 / DSM 3688 / JCM 13448 / NCTC 12043 / ES 2772
RC   {ECO:0000313|Proteomes:UP000010862};
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Peters L., Mikhailova N., Chertkov O.,
RA   Kyrpides N., Mavromatis K., Ivanova N., Brettin T., Detter J.C., Han C.,
RA   Larimer F., Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P.,
RA   Woyke T., Wu D., Gronow S., Wellnitz S., Brambilla E., Klenk H.-P.,
RA   Eisen J.A.;
RT   "Complete sequence of chromosome 2 of Prevotella dentalis DSM 3688.";
RL   Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic
CC         linkages in type I arabinogalactans.; EC=3.2.1.89;
CC         Evidence={ECO:0000256|ARBA:ARBA00001695};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 53 family.
CC       {ECO:0000256|ARBA:ARBA00010687}.
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DR   EMBL; CP003369; AGB28883.1; -; Genomic_DNA.
DR   RefSeq; WP_015310339.1; NZ_GL982489.1.
DR   AlphaFoldDB; L0JDG1; -.
DR   KEGG; pdt:Prede_1577; -.
DR   PATRIC; fig|908937.9.peg.1663; -.
DR   HOGENOM; CLU_294715_0_0_10; -.
DR   Proteomes; UP000010862; Chromosome 2.
DR   GO; GO:0015926; F:glucosidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 2.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR011683; Glyco_hydro_53.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR34983; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1.
DR   PANTHER; PTHR34983:SF1; ARABINOGALACTAN ENDO-BETA-1,4-GALACTANASE A; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   Pfam; PF07745; Glyco_hydro_53; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 2.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000010862};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           20..1028
FT                   /note="arabinogalactan endo-beta-1,4-galactanase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003944380"
FT   DOMAIN          351..700
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
SQ   SEQUENCE   1028 AA;  116432 MW;  8160976F66ACB2D5 CRC64;
     MKKAIFALFL MALSLPAWGQ QRQTFWLGAD ISGTTQLEHA GVALRNARGR VANNVCLQRL
     YGVNAARLRV WVNPENGWCG KDDVLRMAQR AQAHGMAVML DFHYSDSWAD PGHQDIPAAW
     QKMSYGQMRK ALARHTADVL QALKSHGIEV KWVQVGNETT HGFLWPMGRA EENMKQYAGL
     TQAGYDAVKS VYPEAACIVH LDGGCDQERY DRIFDGLRQY GAKWDMIGLS VYPYWDQEAK
     LTSSDEETLQ KAVANINHLY AKYGSESMIV ETGYDADRPV QGREFMKRLI DAAAHQTNGH
     CHGVFYWAPE LEGQYKLGAF RNHRPTVIMD AFREAATMVN ARPAVTWDGL SLMIDGKRVA
     PVMGEIHYSR IPAEEWAREV HKMKLGGITM IACYVFWNHI EEVEGQYDWS GRRSLRDFLE
     VCQLEGLPVI LRLGPFCHGE VRHGGIPDWA LERGVKMRSE NPEFLEMARN LYRQIFTQVQ
     GLQWKDGGPV VAAQFDNEYG GHASYLLSLK KIAKEVGFDL PFYTRTGWPK LADKMPYGEM
     IPLFGDYADG FWDRSVEETA GNYWQAFHFQ PSRANENIGS EQIDYGRQVA ERENADLQYP
     YFTCELGGGM MTSFHRRVYL YPADAYSMAM VKLGSGSNLL GYYMYHGGTN PDGKLTTLNE
     MQRTIATNYN DLPVKTYDFQ APLGEFGQVN PHFFKLRKLH VFMRDFGELL APMAAAFPED
     AVFRKGDDSK LRWNYRHDGD KAFVFVNNYE RLQGLSAKQG VQFTVCGVTF PQRPMVVPAG
     GVAAFPVNLR LGDVRLKYAT AQLLARRERA NGRVAYYFFQ PEGFATEFMV DGKLLGNVRP
     QGTKKAIYKR GNTDFYLLAA AEAESFDLDL DYLKLHSPAA LSVLDEHART VLPQSPGVTV
     AVTKVREARP ERSITVGAAG VAEEPTDEDF EHAAVYLLDL SRIGDWHSGL KVLDIEYQGD
     VARLYCDGKL LDDNFYNGRH FQFGLWRVPE NCRQLELRIL PLQKDMEVYF PQEAKRELGE
     KVISVTVK
//