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Database: UniProt
Entry: L7NK08_PHODP
LinkDB: L7NK08_PHODP
Original site: L7NK08_PHODP 
ID   L7NK08_PHODP            Unreviewed;       208 AA.
AC   L7NK08;
DT   03-APR-2013, integrated into UniProtKB/TrEMBL.
DT   03-APR-2013, sequence version 1.
DT   24-JAN-2024, entry version 63.
DE   RecName: Full=LexA repressor {ECO:0000256|HAMAP-Rule:MF_00015};
DE            EC=3.4.21.88 {ECO:0000256|HAMAP-Rule:MF_00015};
GN   Name=lexA {ECO:0000256|HAMAP-Rule:MF_00015,
GN   ECO:0000313|EMBL:AEU10127.1};
GN   ORFNames=IC627_00525 {ECO:0000313|EMBL:QOD56624.1}, PDP_0386
GN   {ECO:0000313|EMBL:AEU10127.1}, PDPJ_1_01238
GN   {ECO:0000313|EMBL:GAW43824.1};
OS   Photobacterium damsela subsp. piscicida (causative agent of fish
OS   pasteurellosis) (Pasteurella piscicida).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Photobacterium.
OX   NCBI_TaxID=38294 {ECO:0000313|EMBL:AEU10127.1};
RN   [1] {ECO:0000313|EMBL:AEU10127.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=NCIMB 2058 {ECO:0000313|EMBL:AEU10127.1};
RX   PubMed=23219776; DOI=10.1016/j.vaccine.2012.11.064;
RA   Andreoni F., Boiani R., Serafini G., Amagliani G., Dominici S.,
RA   Riccioni G., Zaccone R., Mancuso M., Scapigliati G., Magnani M.;
RT   "Isolation of a novel gene from Photobacterium damselae subsp. piscicida
RT   and analysis of the recombinant antigen as promising vaccine candidate.";
RL   Vaccine 31:820-826(2013).
RN   [2] {ECO:0000313|EMBL:GAW43824.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=OT-51443 {ECO:0000313|EMBL:GAW43824.1};
RA   Aoki T., Teru Y., Morimoto N., Kono T., Sakai M., Takano T., Hawke J.P.,
RA   Fukuda Y., Takeyama H., Hikima J.;
RT   "Complete Genome Sequence of Photobacterium damselae subsp. piscicida
RT   Strain OT-51443 Isolated from Yellowtail (Seriola quinqueradiata) in
RT   Japan.";
RL   Genome Announc. 5:e00404-17(2017).
RN   [3] {ECO:0000313|Proteomes:UP000196133}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OT-51443 {ECO:0000313|Proteomes:UP000196133};
RA   Hikima J., Teru Y., Sakai M., Takano T., Takeyama H., Aoki T.;
RT   "Complete genome sequence of fish pathogenic bacteria, Photobacterium
RT   damsela subsp. piscicida, strain OT-51443, isolated from yellowtail
RT   (Seriola quinqueradiata) in Japan.";
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:QOD56624.1, ECO:0000313|Proteomes:UP000516656}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AS-16-0540-1 {ECO:0000313|EMBL:QOD56624.1,
RC   ECO:0000313|Proteomes:UP000516656};
RA   Baseggio L., Silayeva O., Buller N., Landos M., Engelstaedter J.,
RA   Barnes A.C.;
RT   "Complete, closed and curated genome sequences of Photobacterium damselae
RT   subsp. piscicida isolates from Australia indicate localised evolution and
RT   additional plasmid-borne pathogenicity mechanisms.";
RL   Submitted (SEP-2020) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Represses a number of genes involved in the response to DNA
CC       damage (SOS response), including recA and lexA. In the presence of
CC       single-stranded DNA, RecA interacts with LexA causing an autocatalytic
CC       cleavage which disrupts the DNA-binding part of LexA, leading to
CC       derepression of the SOS regulon and eventually DNA repair.
CC       {ECO:0000256|HAMAP-Rule:MF_00015}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of Ala-|-Gly bond in repressor LexA.; EC=3.4.21.88;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_00015};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00015}.
CC   -!- SIMILARITY: Belongs to the peptidase S24 family.
CC       {ECO:0000256|ARBA:ARBA00007484, ECO:0000256|HAMAP-Rule:MF_00015,
CC       ECO:0000256|RuleBase:RU003991}.
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DR   EMBL; HQ599857; AEU10127.1; -; Genomic_DNA.
DR   EMBL; BDMQ01000001; GAW43824.1; -; Genomic_DNA.
DR   EMBL; CP061854; QOD56624.1; -; Genomic_DNA.
DR   RefSeq; WP_005302475.1; NZ_SUMH01000088.1.
DR   AlphaFoldDB; L7NK08; -.
DR   GeneID; 57340244; -.
DR   Proteomes; UP000196133; Unassembled WGS sequence.
DR   Proteomes; UP000516656; Chromosome 1.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   CDD; cd06529; S24_LexA-like; 1.
DR   Gene3D; 2.10.109.10; Umud Fragment, subunit A; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   HAMAP; MF_00015; LexA; 1.
DR   InterPro; IPR006200; LexA.
DR   InterPro; IPR039418; LexA-like.
DR   InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR   InterPro; IPR006199; LexA_DNA-bd_dom.
DR   InterPro; IPR006197; Peptidase_S24_LexA.
DR   InterPro; IPR015927; Peptidase_S24_S26A/B/C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   NCBIfam; TIGR00498; lexA; 1.
DR   PANTHER; PTHR33516; LEXA REPRESSOR; 1.
DR   PANTHER; PTHR33516:SF2; LEXA REPRESSOR; 1.
DR   Pfam; PF01726; LexA_DNA_bind; 1.
DR   Pfam; PF00717; Peptidase_S24; 1.
DR   PRINTS; PR00726; LEXASERPTASE.
DR   SUPFAM; SSF51306; LexA/Signal peptidase; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE   3: Inferred from homology;
KW   Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813, ECO:0000256|HAMAP-
KW   Rule:MF_00015};
KW   DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW   Rule:MF_00015};
KW   DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW   Rule:MF_00015};
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW   Rule:MF_00015};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW   Rule:MF_00015};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00015};
KW   Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|HAMAP-Rule:MF_00015};
KW   SOS response {ECO:0000256|ARBA:ARBA00023236, ECO:0000256|HAMAP-
KW   Rule:MF_00015};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW   Rule:MF_00015};
KW   Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW   Rule:MF_00015}.
FT   DOMAIN          1..65
FT                   /note="LexA repressor DNA-binding"
FT                   /evidence="ECO:0000259|Pfam:PF01726"
FT   DOMAIN          83..199
FT                   /note="Peptidase S24/S26A/S26B/S26C"
FT                   /evidence="ECO:0000259|Pfam:PF00717"
FT   DNA_BIND        28..48
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00015"
FT   ACT_SITE        125
FT                   /note="For autocatalytic cleavage activity"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00015"
FT   ACT_SITE        162
FT                   /note="For autocatalytic cleavage activity"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00015"
FT   SITE            90..91
FT                   /note="Cleavage; by autolysis"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00015"
SQ   SEQUENCE   208 AA;  22878 MW;  9FE6A1416FA016B7 CRC64;
     MKPLTPRQQE VFDLIKAKIE DSGMPPTRAE IARELGFRSA NAAEEHLKAL ARKGVLEIVP
     GASRGIRLLV GANDSDVEES GLPLIGQVAA GEPILAQEHV ECHYDVDPTL FKPRADFLLR
     VNGMSMKNIG IMDGDLLAVH KTQDVRNGQV VVARVDDDVT VKRLDKQGRK VLLHAENEEF
     DPIVVDLEHQ QLAIEGIAVG VIRNADWM
//
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