ID L8J2E5_9CETA Unreviewed; 4835 AA.
AC L8J2E5;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=M91_07926 {ECO:0000313|EMBL:ELR61602.1};
OS Bos mutus (wild yak).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=72004 {ECO:0000313|EMBL:ELR61602.1, ECO:0000313|Proteomes:UP000011080};
RN [1] {ECO:0000313|EMBL:ELR61602.1, ECO:0000313|Proteomes:UP000011080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=yakQH1 {ECO:0000313|Proteomes:UP000011080};
RX PubMed=22751099; DOI=10.1038/ng.2343;
RA Qiu Q., Zhang G., Ma T., Qian W., Wang J., Ye Z., Cao C., Hu Q., Kim J.,
RA Larkin D.M., Auvil L., Capitanu B., Ma J., Lewin H.A., Qian X., Lang Y.,
RA Zhou R., Wang L., Wang K., Xia J., Liao S., Pan S., Lu X., Hou H., Wang Y.,
RA Zang X., Yin Y., Ma H., Zhang J., Wang Z., Zhang Y., Zhang D., Yonezawa T.,
RA Hasegawa M., Zhong Y., Liu W., Zhang Y., Huang Z., Zhang S., Long R.,
RA Yang H., Wang J., Lenstra J.A., Cooper D.N., Wu Y., Wang J., Shi P.,
RA Wang J., Liu J.;
RT "The yak genome and adaptation to life at high altitude.";
RL Nat. Genet. 44:946-949(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR EMBL; JH880432; ELR61602.1; -; Genomic_DNA.
DR STRING; 72004.ENSBMUP00000014585; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000011080; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd08664; APC10-HERC2; 1.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14402; UBA_HERC2; 1.
DR CDD; cd02344; ZZ_HERC2; 1.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 3.30.60.90; -; 1.
DR Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 2.130.10.30; Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II; 3.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR004939; APC_su10/DOC_dom.
DR InterPro; IPR006624; Beta-propeller_rpt_TECPR.
DR InterPro; IPR021097; CPH_domain.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR037976; HERC2_APC10.
DR InterPro; IPR010606; Mib_Herc2.
DR InterPro; IPR037252; Mib_Herc2_sf.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR InterPro; IPR014722; Rib_uL2_dom2.
DR InterPro; IPR000433; Znf_ZZ.
DR InterPro; IPR043145; Znf_ZZ_sf.
DR InterPro; IPR041987; ZZ_HERC2.
DR PANTHER; PTHR22870; REGULATOR OF CHROMOSOME CONDENSATION; 1.
DR PANTHER; PTHR22870:SF437; X-LINKED RETINITIS PIGMENTOSA GTPASE REGULATOR-RELATED; 1.
DR Pfam; PF03256; ANAPC10; 1.
DR Pfam; PF11515; Cul7; 1.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF06701; MIB_HERC2; 1.
DR Pfam; PF00415; RCC1; 17.
DR Pfam; PF00569; ZZ; 1.
DR PRINTS; PR00633; RCCNDNSATION.
DR SMART; SM01337; APC10; 1.
DR SMART; SM01117; Cyt-b5; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00706; TECPR; 5.
DR SMART; SM00291; ZnF_ZZ; 1.
DR SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF159034; Mib/herc2 domain-like; 1.
DR SUPFAM; SSF50985; RCC1/BLIP-II; 4.
DR SUPFAM; SSF63748; Tudor/PWWP/MBT; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
DR PROSITE; PS51284; DOC; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS51416; MIB_HERC2; 1.
DR PROSITE; PS00626; RCC1_2; 1.
DR PROSITE; PS50012; RCC1_3; 18.
DR PROSITE; PS01357; ZF_ZZ_1; 1.
DR PROSITE; PS50135; ZF_ZZ_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000011080};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00228}.
FT REPEAT 515..570
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 571..622
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 625..676
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 677..728
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT DOMAIN 1208..1284
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000259|PROSITE:PS50255"
FT DOMAIN 1858..1931
FT /note="MIB/HERC2"
FT /evidence="ECO:0000259|PROSITE:PS51416"
FT DOMAIN 2702..2754
FT /note="ZZ-type"
FT /evidence="ECO:0000259|PROSITE:PS50135"
FT DOMAIN 2758..2935
FT /note="DOC"
FT /evidence="ECO:0000259|PROSITE:PS51284"
FT REPEAT 2958..3009
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3010..3064
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3065..3116
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3117..3168
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3171..3222
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3223..3274
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3275..3326
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 3953..4004
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4005..4058
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4059..4110
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4111..4162
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4165..4216
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4217..4268
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 4269..4320
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT DOMAIN 4458..4795
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 51..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1930..1950
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3353..3374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3603..3630
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4805..4835
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..90
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3356..3374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4809..4823
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4763
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4835 AA; 526884 MW; CD1C4658FA41DAA7 CRC64;
MPSDSFCLAA QARLDSKWLK TDIQLAFTRD GLCGLWNEMV KDGEIVYTGT ELSQNGELPP
RKDDGVDTQI GTKKEDLNDK EKKEEEETPA PAYRAKSILE SWVWGKQPDV NELKECLSVL
VKEQQALAIQ SATTTLSALR LKQRLIVLER YFIALNRTVF QENVKVKWKS SNISLPPVDK
KSSRPAGKGV EGLARVGSRA ALSFAFAFLR RAWRSGEDAD LCSELLQESL DALRALPEAS
LFDESTVSSV WLEVVERATR FLRSVVTGDV HGTPGTKGPG SIPLQDQHLA LAILLELAVQ
RGALSQMLSA VLLLLHLWDS GTRETDNERS AQGTSAPLLP LLRRFRSIVC SKDGPCAEVD
THLLSGPLSP NESFLRYLTL PQDNELAIDL RQTAVVVMAH LDRLATPCMP PLCSSPTSHK
GSLQEVIGWG LIGWKYYANV IGPIQCEGLA NLGVTQIACA EKRFLILSRN GRVYTQAYNS
DTLAPQLVQG LASRNIVKIA AHSDGHHYLA LAATGEVYSW GCGDGGRLGH GDTVPLEEPK
VISAFSGKQA GKHVVHIACG STYSAAITAE GELYTWGRGN YGRLGHGSSE DEAIPMLVAG
LKGLKVIDVA CGSGDAQTLA VTENGQVWSW GDGDYGKLGR GGSDGCKTPK LIEKLQDLDV
VKVRCGSQFS VALTKDGQVY SWGKGDNQRL GHGTEEHVRY PKLLEGLQGK KVIDVAAGST
HCLALTEDSE VHSWGSNDQC QHFDTLRVTK PEPSALPGLD TKHIVGIACG PAQSFAWSSC
SEWSIGLRVP FVVDICSMTF EQLDLLLRQV SEGMDGTADW PPPQEKEWMT VATLNLLRLQ
LHAAISHQVD PEFLGLGLGS VLLNSLKQTV VALASSAGVL STVQAAAQAV LQSGWSVLLP
TAEERARALS ALLPCAVSGN EVNISPGRRF MIDLLVGSLM ADGGLESALN AAITAEIQDI
EAKKEAQKEK EIDEQEANAS TFHRSRTPLD KDLINTGIYE SSGKQCLPLV QLIQQLLRNI
ASQTVARLKD VARRISSCLD FEQHSRERSA SLDLLLRFQR LLISKLYPGE SVGQTSEISS
PELMGVGSLL KKYTALLCTH IGDILPVAAS IASTSWRHFA EVACIVEGDF TGILLPELVV
SIVLLLSKNA GLMQEAGAVP LLGGLLEHLD RFNHLAPGKE RDDLEELAWP GIMESFFTGQ
NCRNNEEVTL IRKADLENHN KDGGFWTVID GKVYDIKDFQ TQSLTGNSIL AQFVGEDPVV
ALEAALQFED TRESMHAFCV GQYLEPDQEV VTIPDLGSLS SPLIDTERNL GLLLGLHASY
LAMSTPLSPV EVECAKWLQS SIFSGGLQTS QIHYSYNEEK DEDHCSSPGG TPASKSRLCS
HRQALGDHAQ AFPIADNNIQ DHNVKDFLCQ IERYCRQCHL TTPITFPPEH PVEEVGRLLL
CCLLKHEDLG HVALSLVHAG TLGIEQVKHR TLPKSVVDVC RVVYQAKCSL IKTHQEQGRS
YKEVCAPVIE RLRFLFNELR PAVCNDLSIM SKFKLLSSLP RWKRIAQKII RERRKKRVPK
KPESAEEEEK IGNGESDLEE ACILPHSPIN VDKRPITIKS PKDKWQPLLS TVTGVHRYKW
LKQNVQGLYP QSALLSTIAE FALKEEPVDV EKMRKCLLKQ LERAEVRLEG IDTILKLAAK
NFLLPSVQYA MFCGWQRLIP EGIDIGEPLT DCLKDVDLIP PFNRMLLEVT FGKLYTWAVQ
NIRNVLIDAN TKFKELGIQP VPLQTITNEN PSGPSLGTTP QARFLLVMLS MLTLQHGAHN
LSLLLNSGTL ALTQTALRLI GPSCDNVEED MNASARGASA TVLEETRKET APVQLPVSGP
ELAAMMKIGT RVMRGVDWKW GDQDGPPPGL GRVIGELGED GWIRVQWDTG STNSYRMGKE
GKYDLKLAEL PVSTQPSAED SDTEDDSEAE QIERNIHPTA MMLTSTVNLL QTLCLSAGVH
AEVMQSEATK TLCGLLRMLV ESGTTDKTSS PSRLVCREQH RSWCTLGFVR SIALTPHMCA
ALSSPPWISL LVKVVEGHSP FTAASLQRQI LAVHLLQAVL PSWDKTERAR DMECLVEKLF
GFLGSLLTTC SSDVPFLRES TLRRRRARPQ ASLTATHSST LAEEVVALLR TLHSLGQWNG
LINKYINSQL HSVTRSCAGK PAEGALLDDY FPDCENPEVG SLMAVLAVIG GIDGRLRLGG
QVMHDDFGEG TVTRITPKGK ITVQFCDLRT CRVCPLNQLK PLPAVAFSVT NLPFTEPMLS
VWAQLVNLAG SKLEKHKIKK SPKQGFAGQV DLDLLRCQQL KLYILKAGRA LLSHQDQLRQ
ILSQPAVQEA AAAHTDDGAA VSPDLVDMSP EGPQPPMILL QQLLASATQP SPVKAIFDKQ
ELEAAALAVC QCLAVESTHP LSPVFEDCSS SEATTPVPVQ HIRPVRAKKH KQSPGPVLPI
VVQLMEMGFP RRNIEFALKS LTGASGNAAG LPGVEALVGW LLDHADVQVT DLSDADTGSE
ECSDEEVVED VDDTAYAMST GAVVTESQTY KKRADFLSND DYAVYVRENI QVGMMVRCCR
TYEEVCEGDV GKVIKLDRDG LHDLNVQCDW QQKGGTYWVR YIHVELIGYP PPSSPSHIKI
GDKVRVKASV TTPKYKWGSV THQSVGVVKA FSANGKDVIV DFPQQSHWTG LLSEMELVPS
VHPGVTCDGC QMFPINGSRF KCRNCDDFDF CEACFKTRKH NTRHTFGRIN EPGQSAIFCG
RSGKQLKRCH NSQPGMLLDS WARMVKSLNV SSSVNQASRL IDGSEPCWQS SGSQGKHWIR
LEIFPDVLVH RLKMIVDPAD SSYMPSLVVV SGGNSLNNLI ELKTININPT DTTVSLLNDC
TEYHRYIEIA IKQCRSSGID CKIHGLILLG RIRAEEEDMA AVPFLASDNE EEEDEKGNSG
SLIRKKAAGL ESAATIRTKV FVWGLNDKDQ LGGLKGSKIK VPSFSETLSA LNVVQVAGGS
KSLFAVTVEG KVYACGEATN GRLGLGISSG TVPIPRQITA LSSYVVKKVA VHSGGRHATA
LTVDGKVFSW GEGDDGKLGH FSRMNCDKPR LIEALKTKRI RDIACGSSHS AALTSSGELY
TWGLGEYGRL GHGDNTTQLK PKMVKVLLGH RVIQVACGSR DAQTLALTDE GLVFSWGDGD
FGKLGRGGSE GCNIPQNIER LNGQGVCQIE CGAQFSLALT KSGVVWTWGK GDYFRLGHGS
DVHVRKPQVV EGLRGKKIVH VAVGALHCLA VTDAGQVYAW GDNDHGQQGN GTTTVNRKPT
LVQGLEGQKI TRVACGSSHS VAWTTVDVAT PSVHEPVLFQ TARDPLGASY LGVPSDADSS
PASNKINGAN NSKPNRPSLA KILLSLDGNL AKQQALSHIL TALQIMYARD TVVGALMPAG
MVAPVECPSF SSSAPASDVS AMASPMNGEE CVLAVDIEDR LSPNPWQEKR GEIISSEDAV
TPSAVTPSAC SASSRPFIPV TDDPGAASII AETMTKTKED VESQNKVSGP ELQSLDEFTS
LLVVDDTRVL VDLLKLSVCG RAGDKGRDVL SAVLSGMGTA YPQVADMLLE LCVTELEDVA
TDSQSGRLSS QPVVVESSHP YTDDTSSSGT VKIPGAEGLR VEFDRQCSTE RRHDPLTVMD
GVNRIVSVRS GREWSDWSSE LRIPGDELKW KFISDGSVNG WGWRFTVYPI MPAAGPKDLL
SDRCVLSCPS MDLVTCLLDF RLNLASNRSI IPRLAASLAA CAQLSALAAS HRMWALQRLR
KLLTTEFGQS ININRLLGDN DGETRALSFS GSALAALVKG LPEALQRQFE YEDPIVRGGK
QLLHSPFFKV LVALACDLEL DTLPCCAETH KWAWFRRYCM ASRVAVALDK RTSLPRLFLD
EVAKKIRELM ADGENMDVLH ESHSIFRREQ DEQLVQWMNR RPDDWTLSAG GSGTIYGWGH
NHRGQLGGIE GAKVKVPTPC EALAALRPVQ LIGGEQTLFA VTADGKLYAT GYGAGGRLGV
GGTESVSTPT LLESIQHVFI KKVAVNSGGK HCLALSSEGE VYSWGEAEDG KLGHGNRSPC
DRPRVIESLR GTEVVDVAAG GAHSACVTAA GDLYTWGKGR YGRLGHSDSE DQLKPKLVEA
LQGHRVVDVA CGSGDAQTLC LTDDDTVWSW GDGDYGKLGR GGSDGCKVPM KIDSLTGLGV
VKVECGSQFS VALTKSGAVY TWGKGDYHRL GHGSDDHVRR PRQVQGLQGK KVIAIATGSL
HCVCCSEDGE VYTWGDNDEG QLGDGTTNAI QRPRLVAALQ GKKVNRVACG SAHTLAWSTS
KPASAGKLPV QVPMEYNHLQ EIPIIALRNR LLLLHHISEL FCPCIPMFDL EGSLDETGLG
PSVGFDTLRG ILISQGKEAA FRKVVQATMV RDRQHGPVVE LNRIQVKRSR SKGGLAGPDG
TKSVFGQMCA KMTSFSPDSL LLPHRVWKVK FVGESVDDCG GGYSESIAEI CEELQNGLTP
LLIVTPNGRD ESGANRDCYL FSPAARAPVH TNMFRFLGVL LGIAIRTGSP LSLNLAEPVW
KQLAGMSLTI ADLSEVDKDF IPGLMYIRDN EATSEEFEAM SLPFTVPSAS GQDIQLSSKY
THITLDNRAE YVRLAINYRL HEFDEQVAAV REGMARVVPV PLLSLFTGYE LETMVCGSPD
IPLHLLKSVA TYKGVEPSAP LIQWFWEVME SFSNTERSLF LRFVWGRTRL PRTIADFRGR
DFVIQVLDKY NPPDHFLPES YTCFFLLKLP RYSCKQVLEE KLKYAIHFCK SIDTDDYARI
ALTGEPAADD SSDDSENEDV DSFASDSTQD YLTGH
//
