ID L8LJP9_9CHRO Unreviewed; 956 AA.
AC L8LJP9;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=glycerophosphodiester phosphodiesterase {ECO:0000256|ARBA:ARBA00012247};
DE EC=3.1.4.46 {ECO:0000256|ARBA:ARBA00012247};
DE Flags: Fragment;
GN ORFNames=GLO73106DRAFT_00015780 {ECO:0000313|EMBL:ELR97763.1};
OS Gloeocapsa sp. PCC 73106.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
OC Chroococcales; Chroococcaceae; Gloeocapsa.
OX NCBI_TaxID=102232 {ECO:0000313|EMBL:ELR97763.1, ECO:0000313|Proteomes:UP000011180};
RN [1] {ECO:0000313|EMBL:ELR97763.1, ECO:0000313|Proteomes:UP000011180}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 73106 {ECO:0000313|EMBL:ELR97763.1,
RC ECO:0000313|Proteomes:UP000011180};
RX PubMed=23277585; DOI=10.1073/pnas.1217107110;
RA Shih P.M., Wu D., Latifi A., Axen S.D., Fewer D.P., Talla E., Calteau A.,
RA Cai F., Tandeau de Marsac N., Rippka R., Herdman M., Sivonen K.,
RA Coursin T., Laurent T., Goodwin L., Nolan M., Davenport K.W., Han C.S.,
RA Rubin E.M., Eisen J.A., Woyke T., Gugger M., Kerfeld C.A.;
RT "Improving the coverage of the cyanobacterial phylum using diversity-driven
RT genome sequencing.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:1053-1058(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a sn-glycero-3-phosphodiester + H2O = an alcohol + H(+) + sn-
CC glycerol 3-phosphate; Xref=Rhea:RHEA:12969, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30879, ChEBI:CHEBI:57597,
CC ChEBI:CHEBI:83408; EC=3.1.4.46;
CC Evidence={ECO:0000256|ARBA:ARBA00029315};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 1 Mg(2+) ion. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 2 Zn(2+) ions. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ELR97763.1}.
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DR EMBL; ALVY01000204; ELR97763.1; -; Genomic_DNA.
DR AlphaFoldDB; L8LJP9; -.
DR STRING; 102232.GLO73106DRAFT_00015780; -.
DR eggNOG; COG0584; Bacteria.
DR eggNOG; COG1785; Bacteria.
DR eggNOG; COG4222; Bacteria.
DR Proteomes; UP000011180; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016791; F:phosphatase activity; IEA:InterPro.
DR GO; GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 2.
DR Gene3D; 3.20.20.190; Phosphatidylinositol (PI) phosphodiesterase; 1.
DR InterPro; IPR001952; Alkaline_phosphatase.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR030395; GP_PDE_dom.
DR InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR PANTHER; PTHR43620:SF7; GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPD6; 1.
DR PANTHER; PTHR43620; GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE; 1.
DR Pfam; PF00245; Alk_phosphatase; 2.
DR Pfam; PF03009; GDPD; 1.
DR SMART; SM00098; alkPPc; 1.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 2.
DR SUPFAM; SSF51695; PLC-like phosphodiesterases; 1.
DR PROSITE; PS51704; GP_PDE; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601952-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601952-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000011180};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Zinc {ECO:0000256|PIRSR:PIRSR601952-2}.
FT DOMAIN 12..373
FT /note="GP-PDE"
FT /evidence="ECO:0000259|PROSITE:PS51704"
FT ACT_SITE 430
FT /note="Phosphoserine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-1"
FT BINDING 386
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 386
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 661
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 666
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 708
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT NON_TER 956
FT /evidence="ECO:0000313|EMBL:ELR97763.1"
SQ SEQUENCE 956 AA; 103699 MW; 83B4A12A504860DC CRC64;
MNNFNTLTGE RPLIIAHRGA SGVLPEHTLE AYELAIEQGA DFVEPDLVIT SDGVLIARHE
PYLDDTSNVA EVFGEERKAT KMVDGREITA YFAEDFTLAE IKQLKARQNT LTFPERSREF
DDLFEIPTLE EIIDLVQRVE AETGKEVGIY PETKQPTFFQ QQGLPLEEPL VETFLSKGFT
NPERIFFQSF EVANLIRLKQ ELLPGTELEN TPLVQLYSRF ERQPYDIIHN FSDPNFDPMT
VYGTDAITAT TTYGDLINQD NNPNVNLLAD FVANYAEGIG PWKRTFVLTE PLDPEQQLGA
VTVQLTGEVL PVVEDAHEAG LQVHPYTFRD EESALALEQD GTIQTPEEEL QQHFELGVDG
VFVDHPDTAI DALNNLNRHV IFIHPDGTSP SHYAAARFVE HGPDGRLNWD QLDQAAVYLG
HLEDQLGGTS NAGAVTHATG AKVYAESFGF NADNSPVESL SGNTGRTIMH EAIEAEKVTA
LVQSGFAAEP GTAAFVAQVP EIEIDGQRIP PRQQIAEITK QVIESGVDFI MGGGELHMLP
IGTPGFHATA AQMDALSTNP LNRPTENLIE LAESLGYTVV YNRDQLLDLL ENPTPPTKVL
GVFATLHTFN DRPEEVLGLG TPQETPNFDP DAPTIAEMLS VTQTLAEQHP NFGNGSFVVV
EEEGSDNLAN NNNASGTIEA TLRADEAIGV ALDFNERYPN SLIVTAADSD AGGLQVRDPV
AADTNVGNIN NNPTLERRDI PLDGQTGSNT QPFVSAPDAS GDELPFGIAW AGLPDFSGGI
VAKAHGVNSE NLPATVDNTG IYELMYETLF ETELTSRIES APVAPEATEE TGNVIFIHPD
GTSANHYMAL RNIDYGPDGR LNWDDMSNAG VYLGHMEDQL TGTSNGGAVT HATGVKVFAE
SFGFNEDNSP ITSASGMTGM TIMEEAIEAG KATALVQSGH IAEPGTAAFV AQTLNR
//
