ID LEUD_PYRHO Reviewed; 163 AA.
AC O59393;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 27-MAR-2024, entry version 134.
DE RecName: Full=3-isopropylmalate dehydratase small subunit;
DE EC=4.2.1.33;
DE AltName: Full=Alpha-IPM isomerase;
DE Short=IPMI;
DE AltName: Full=Isopropylmalate isomerase;
GN Name=leuD; OrderedLocusNames=PH1724;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC isopropylmalate, via the formation of 2-isopropylmaleate.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC EC=4.2.1.33;
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 2/4.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 2 subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000001; BAA30838.1; -; Genomic_DNA.
DR PIR; G71180; G71180.
DR RefSeq; WP_010885788.1; NC_000961.1.
DR PDB; 1V7L; X-ray; 1.98 A; A/B=1-163.
DR PDBsum; 1V7L; -.
DR AlphaFoldDB; O59393; -.
DR SMR; O59393; -.
DR STRING; 70601.gene:9378720; -.
DR EnsemblBacteria; BAA30838; BAA30838; BAA30838.
DR GeneID; 1442569; -.
DR KEGG; pho:PH1724; -.
DR eggNOG; arCOG02230; Archaea.
DR OrthoDB; 6505at2157; -.
DR BRENDA; 4.2.1.33; 5244.
DR UniPathway; UPA00048; UER00071.
DR EvolutionaryTrace; O59393; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01577; IPMI_Swivel; 1.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR HAMAP; MF_01032; LeuD_type2; 1.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR033940; IPMI_Swivel.
DR InterPro; IPR011827; LeuD_type2/HacB/DmdB.
DR NCBIfam; TIGR02087; LEUD_arch; 1.
DR PANTHER; PTHR43345:SF2; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 1-RELATED; 1.
DR PANTHER; PTHR43345; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amino-acid biosynthesis;
KW Branched-chain amino acid biosynthesis; Leucine biosynthesis; Lyase.
FT CHAIN 1..163
FT /note="3-isopropylmalate dehydratase small subunit"
FT /id="PRO_0000141949"
FT STRAND 2..9
FT /evidence="ECO:0007829|PDB:1V7L"
FT HELIX 16..19
FT /evidence="ECO:0007829|PDB:1V7L"
FT HELIX 30..36
FT /evidence="ECO:0007829|PDB:1V7L"
FT TURN 37..41
FT /evidence="ECO:0007829|PDB:1V7L"
FT HELIX 45..48
FT /evidence="ECO:0007829|PDB:1V7L"
FT STRAND 54..56
FT /evidence="ECO:0007829|PDB:1V7L"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:1V7L"
FT HELIX 69..77
FT /evidence="ECO:0007829|PDB:1V7L"
FT STRAND 81..85
FT /evidence="ECO:0007829|PDB:1V7L"
FT HELIX 89..98
FT /evidence="ECO:0007829|PDB:1V7L"
FT STRAND 102..105
FT /evidence="ECO:0007829|PDB:1V7L"
FT STRAND 115..119
FT /evidence="ECO:0007829|PDB:1V7L"
FT TURN 120..123
FT /evidence="ECO:0007829|PDB:1V7L"
FT STRAND 124..127
FT /evidence="ECO:0007829|PDB:1V7L"
FT STRAND 130..133
FT /evidence="ECO:0007829|PDB:1V7L"
FT HELIX 139..146
FT /evidence="ECO:0007829|PDB:1V7L"
FT HELIX 150..156
FT /evidence="ECO:0007829|PDB:1V7L"
SQ SEQUENCE 163 AA; 18011 MW; AEFBDDE1CBCDD266 CRC64;
MITTGKVWKF GDDISTDEIT PGRYNLTKDP KELAKIAFIE VRPDFARNVR PGDVVVAGKN
FGIGSSRESA ALALKALGIA GVIAESFGRI FYRNAINIGI PLLLGKTEGL KDGDLVTVNW
ETGEVRKGDE ILMFEPLEDF LLEIVREGGI LEYIRRRGDL CIR
//
