ID LIP4_ARATH Reviewed; 373 AA.
AC Q9FXB6; O82076;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE RecName: Full=GDSL esterase/lipase LIP-4;
DE EC=3.1.1.-;
DE AltName: Full=Extracellular lipase LIP-4;
DE Flags: Precursor;
GN Name=LIP4; OrderedLocusNames=At1g56670; ORFNames=F25P12.90;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 45-345.
RC STRAIN=cv. Columbia;
RA Dickstein R., Davies P., Hoogland D., Brick D., Upton C.;
RT "An Arabidopsis lipase-like gene within a sequence previously characterized
RT as an intron.";
RL J. Biochem. Mol. Biol. Biophys. 1:265-273(1998).
RN [4]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AC009323; AAG09098.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33421.1; -; Genomic_DNA.
DR EMBL; U57828; AAC23651.1; -; mRNA.
DR PIR; D96608; D96608.
DR PIR; T52366; T52366.
DR RefSeq; NP_176059.1; NM_104543.4.
DR AlphaFoldDB; Q9FXB6; -.
DR SMR; Q9FXB6; -.
DR STRING; 3702.Q9FXB6; -.
DR GlyCosmos; Q9FXB6; 1 site, No reported glycans.
DR PaxDb; 3702-AT1G56670-1; -.
DR ProteomicsDB; 238439; -.
DR EnsemblPlants; AT1G56670.1; AT1G56670.1; AT1G56670.
DR GeneID; 842122; -.
DR Gramene; AT1G56670.1; AT1G56670.1; AT1G56670.
DR KEGG; ath:AT1G56670; -.
DR Araport; AT1G56670; -.
DR TAIR; AT1G56670; -.
DR eggNOG; ENOG502QTD0; Eukaryota.
DR HOGENOM; CLU_015101_2_0_1; -.
DR InParanoid; Q9FXB6; -.
DR OMA; MHYSKPP; -.
DR OrthoDB; 458055at2759; -.
DR PhylomeDB; Q9FXB6; -.
DR BioCyc; ARA:AT1G56670-MONOMER; -.
DR PRO; PR:Q9FXB6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FXB6; baseline and differential.
DR Genevisible; Q9FXB6; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; SGNH hydrolase; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR PANTHER; PTHR22835:SF275; GDSL ESTERASE_LIPASE LIP-4; 1.
DR PANTHER; PTHR22835; ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN; 1.
DR Pfam; PF00657; Lipase_GDSL; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..32
FT /evidence="ECO:0000255"
FT CHAIN 33..373
FT /note="GDSL esterase/lipase LIP-4"
FT /id="PRO_0000367341"
FT ACT_SITE 47
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 339
FT /evidence="ECO:0000250"
FT ACT_SITE 342
FT /evidence="ECO:0000250"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 112
FT /note="G -> V (in Ref. 3; AAC23651)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="P -> S (in Ref. 3; AAC23651)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="R -> L (in Ref. 3; AAC23651)"
FT /evidence="ECO:0000305"
FT CONFLICT 214
FT /note="G -> E (in Ref. 3; AAC23651)"
FT /evidence="ECO:0000305"
FT CONFLICT 244
FT /note="H -> L (in Ref. 3; AAC23651)"
FT /evidence="ECO:0000305"
FT CONFLICT 330
FT /note="E -> K (in Ref. 3; AAC23651)"
FT /evidence="ECO:0000305"
FT CONFLICT 345
FT /note="E -> G (in Ref. 3; AAC23651)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 373 AA; 41576 MW; 1D9352A9E66C77DC CRC64;
MATLFLYSNT FSFFFITLVS LALLILRQPS RAASCTARPV IFNFGDSNSD TGGLVAGLGY
PIGFPNGRLF FRRSTGRLSD GRLLIDFLCQ SLNTSLLRPY LDSLGRTRFQ NGANFAIAGS
PTLPKNVPFS LNIQVKQFSH FKSRSLELAS SSNSLKGMFI SNNGFKNALY MIDIGQNDIA
RSFARGNSYS QTVKLIPQII TEIKSSIKRL YDEGGRRFWI HNTGPLGCLP QKLSMVKSKD
LDQHGCLVSY NSAATLFNQG LDHMCEELRT ELRDATIIYI DIYAIKYSLI ANSNQYGFKS
PLMACCGYGG TPYNYNVKIT CGHKGSNVCE EGSRFISWDG IHYTETANAI VAMKVLSMHY
SKPPTPFHFF CRR
//
