ID M0AWW5_NATA1 Unreviewed; 957 AA.
AC M0AWW5;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN ORFNames=C481_07331 {ECO:0000313|EMBL:ELZ02463.1};
OS Natrialba asiatica (strain ATCC 700177 / DSM 12278 / JCM 9576 / FERM
OS P-10747 / NBRC 102637 / 172P1).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales;
OC Natrialbaceae; Natrialba.
OX NCBI_TaxID=29540 {ECO:0000313|EMBL:ELZ02463.1, ECO:0000313|Proteomes:UP000011554};
RN [1] {ECO:0000313|EMBL:ELZ02463.1, ECO:0000313|Proteomes:UP000011554}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12278 {ECO:0000313|EMBL:ELZ02463.1,
RC ECO:0000313|Proteomes:UP000011554};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ELZ02463.1}.
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DR EMBL; AOIO01000021; ELZ02463.1; -; Genomic_DNA.
DR AlphaFoldDB; M0AWW5; -.
DR STRING; 29540.C481_07331; -.
DR PATRIC; fig|29540.5.peg.1493; -.
DR eggNOG; arCOG00795; Archaea.
DR Proteomes; UP000011554; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR014808; DNA_replication_fac_Dna2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR43788; DNA2/NAM7 HELICASE FAMILY MEMBER; 1.
DR PANTHER; PTHR43788:SF8; HELICASE WITH ZINC FINGER 2; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF08696; Dna2; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:ELZ02463.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:ELZ02463.1};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000011554}.
FT DOMAIN 55..246
FT /note="DNA replication factor Dna2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08696"
FT DOMAIN 547..657
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 666..733
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 754..939
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
SQ SEQUENCE 957 AA; 104942 MW; 01BC7CCA76F583E9 CRC64;
MSTSYGESEL AEVPVRLAVE SDRLGDVTAT TSPFDAVETE TDGGTLSDGA ARAADSTTIT
LWNKWTESAE LLEPGMELLV TDAEEDEFRG EQRYATTGDS YVVVAPSFHV NVTAIRNWVE
CPRLYYLNKL SGVPLNYPVV KGTLVHEVFG DLLRGRDLEE SIDARVEERG LELGLLGETA
DAVAEDVREN AKAIEGWLEQ GRLTEEERAT AADNSERAFT PAESSWRSEQ LLISETFGIR
GRADAIRRGA PVELKTGKNL KKEPRFKDKV QAACYALLLE EHGGDVDTGT LLYTKNSALD
RNEETGDLTP AKEFSMGDGL LKFVVRLRNE LAAMEVAGDV PTGYEGSAKC EYCFEQDTCM
VVSGRLDQES KAGQIGQALP AEEREYFERF SRAIEAERRA VHREFAKLWE QDARERADDD
RALIDLAFVE STELAEGRWE LRARRPGGAT SKLREGDLVL ASDGHPVRGS AELARIERLD
EDELVLTADE PVEVTRLDVY PSELTTDRLL AALHDALLKG DERRKDVLFD RAEPAFEPLE
ETFIPNNDAQ DEAVRKAVGA DDCALIHGPP GTGKTYTIAR AIRAMVERGE RVLLSAFTNR
AVDNALEALL DQLEGEIGED SVASPAQRNG DGEAVDVVRV GSESGVREDM QPYRLDRAGE
PGERAATLQN AQVVAATTAT CGSRVMKEQR FDAALVDEAA QLTEPGTHAA INLADWFVLV
GDHEQLPPVV QSEGRTRRVS SGANGDEPRA ADLTESLFER LVDLYPGAGV MLTKQYRMNQ
RIQAFASQEF YDGELRPATG DVAGRTLDDL SGVTRDDLPT ELRGAVTFVD VDGDGSRYTD
SEEAARIAEM VEEFRAAGLS PADIGVIAPF RAQVSELSKH VPAEVAVDTV DRFQGSSQEV
IIVSFTATDS LEGPIFDDYR RINVALTRPK RALVLVGDAN ALATDPVYSR MLEWARR
//
