ID M0JA66_9EURY Unreviewed; 149 AA.
AC M0JA66;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=Crossover junction endodeoxyribonuclease Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000256|HAMAP-Rule:MF_01490};
DE AltName: Full=Holliday junction resolvase Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN ORFNames=C438_08612 {ECO:0000313|EMBL:EMA05876.1};
OS Haloferax denitrificans ATCC 35960.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=662478 {ECO:0000313|EMBL:EMA05876.1, ECO:0000313|Proteomes:UP000011553};
RN [1] {ECO:0000313|EMBL:EMA05876.1, ECO:0000313|Proteomes:UP000011553}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35960 {ECO:0000313|EMBL:EMA05876.1,
RC ECO:0000313|Proteomes:UP000011553};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are
CC subsequently ligated to produce recombinant products.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10;
CC Evidence={ECO:0000256|ARBA:ARBA00029354, ECO:0000256|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01490};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EMA05876.1}.
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DR EMBL; AOLP01000009; EMA05876.1; -; Genomic_DNA.
DR AlphaFoldDB; M0JA66; -.
DR PATRIC; fig|662478.6.peg.1653; -.
DR Proteomes; UP000011553; Unassembled WGS sequence.
DR GO; GO:0008821; F:crossover junction DNA endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; Holliday_junction_resolvase; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR NCBIfam; NF040854; Hol_resolv_Hjc; 1.
DR PANTHER; PTHR39651; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR PANTHER; PTHR39651:SF1; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_01490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_01490}.
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 40
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 20
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 50
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 63
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT SITE 65
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
SQ SEQUENCE 149 AA; 16370 MW; 1D49877B6115C9BB CRC64;
MTARGRLPTM SSNRKGDRRE RELVNALDEA GFAVMRAPAS GSATTRELPD VLAGNGEVFY
AIEAKSSSGR PIYLSGEEVE ALVYFSRNFG AKARIAVRFD REDWYFFHPG DLYVTDGGNY
RVKKETALAE GEDFESFTGG PTQTKLGGD
//
