ID M3YEC8_MUSPF Unreviewed; 3695 AA.
AC M3YEC8;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 52.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HUWE1 {ECO:0000313|Ensembl:ENSMPUP00000009685.1};
OS Mustela putorius furo (European domestic ferret) (Mustela furo).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Musteloidea; Mustelidae;
OC Mustelinae; Mustela.
OX NCBI_TaxID=9669 {ECO:0000313|Ensembl:ENSMPUP00000009685.1};
RN [1] {ECO:0000313|Ensembl:ENSMPUP00000009685.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (MAR-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; AEYP01101822; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01101823; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01101824; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01101825; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01101826; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01101827; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01101828; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01101829; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR Ensembl; ENSMPUT00000009841.1; ENSMPUP00000009685.1; ENSMPUG00000009760.1.
DR GeneTree; ENSGT00940000156319; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14288; UBA_HUWE1; 1.
DR Gene3D; 3.30.720.50; -; 1.
DR Gene3D; 6.10.250.1630; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR041918; UBA_HUWE1.
DR InterPro; IPR004170; WWE-dom.
DR InterPro; IPR037197; WWE_dom_sf.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR Pfam; PF02825; WWE; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR SUPFAM; SSF117839; WWE domain; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS50918; WWE; 1.
PE 4: Predicted;
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 650..689
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 937..1014
FT /note="WWE"
FT /evidence="ECO:0000259|PROSITE:PS50918"
FT DOMAIN 3359..3695
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 37..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 312..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 348..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 623..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 730..749
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1023..1052
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1352..1399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1596..1676
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1690..1813
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2028..2304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2370..2393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2575..2601
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2688..2709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2740..2760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2806..2846
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2873..2902
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3073..3094
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3115..3169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 623..644
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1033..1052
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1352..1369
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1370..1394
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1596..1629
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1718..1736
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1740..1805
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2028..2052
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2053..2112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2149..2225
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2238..2268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2375..2393
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2578..2594
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2806..2843
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2873..2891
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3134..3151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3662
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3695 AA; 405359 MW; CBDE8AFA489FEB82 CRC64;
MRHQPTLKTD ATTAIIKLLE EICNLGRDPK YICQKPSIQK ADGTAAAPPP RSNHAAEEAS
SEDEEEEEVQ AMQSFNSTQQ NETEPNQQVV GTEERIPIPL MDYILNVMKF VESILSNNTT
DDHCQEFVNQ KGLLPLVTIL GLPNLPIDFP TSAACQAVAG VCKSILTLSH EPKVLQEGLL
QLDSILSSLE PLHRPIESPG GSVLLRELAC AGNVADATLS AQATPLLHAL TAAHAYIMMF
VHTCRVGQSE IRSISVNQWG SQLGLSVLSK LSQLYCSLVW ESTVLLSLCT PNSLPSGCEF
GQADMQKLVP KDEKAGTTQG GKRSDGEQDG TAASMDASTQ GLLEGIGLDG DTLAPMETDE
PTASDSKGKS KITPAMAARI KQIKPLLSAS SRLGRALAEL FGLLVKLCVG SPVRQRRSHH
AASTTTAPTP AARSTASALT KLLTKGLSWQ PPPYTPTPRF RLTFFICSVG FTSPMLFDER
KYPYHLMLQK FLCSGGHNAL FETFNWALSM GGKVPVAEGL EHSDLPDGTG EFLDAWLMLV
EKMVNPTTVL ESPHSLPAKL PGGVQNFPQF SALRFLVVTQ KAAFTCIKNL WNRKPLKVYG
GRMAESMLAI LCHILRGEPV IREKLSKEKE GSRGEEDTGQ EEGGSRREPQ VNQQQLQQLM
DMGFTREHAM EALLNTSTME QATEYLLTHP PPIMGGVVRD LSMSEEDQMM RAIAMSLGQD
IPMDQRAESP EEVACRKEEE ERKAREKQEE EEAKCLEKFQ DADPLEQDEL HTFTDTMLPG
CFHLLDELPD TVYRVCDLIM TAIKRNGADY RDMILKQVVN QVWEAADVLI KAALPLTTSD
TKTVSEWISQ MATLPQASNL ATRILLLTLL FEELKLPCAW VVESSGILNV LIKLLEVVQP
CLQAAKEQKE VQTPKWITPV LLLIDFYEKT AISSKRRAQM TKYLQSNNNN WRWFDDRSGR
WCSYSASNNS TIDSAWKSGE TSVRFTAGRR RYTVQFTTMV QVNEETGNRR PVMLTLLRVP
RLNKNSKNSN GQELEKTLEE SKEMDIKRKE NKANDTPLAL EGTNTEKETS LEETKIGEIL
IQGLTEDMVT VLIRACVSML GVPVDPDTLH ATLRLCLRLT RDHKYAMMFA ELKSTRMILN
LTQSSGFNGF TPLVTLLLRH IIEDPCTLRH TMEKVVRSAA TSGAGSTTSG VVSGSLGSRE
INYILRVLGP AACRNPDIFT EVANCCIRIA LPAPRGSGTA SDDEFENLRI KGPNAVQLVK
TTPLKPSPLP VIPDTIKEVI YDMLNALAAY HAPEEAEKSD PKPGGMTQEV GQLLQDMGDD
VYQQYRSLTR QSSDFDTQSG FSINSQVFAA DGASTETSTS GTSQGEASTP EESRDGKKDK
EGDRASEEGK QKGKGSKPLM PTSTILRLLA ELVRSYVGIA TLIANYSYTV GQSELIKEDC
SVLAFVLDHL LPHTQNAEDK DTPALARLFL ASLAAAGSGT DAQVALVNEV KAALGRALAM
AESTEKHARL QAVMCIISTI MESCPSTSSF YSSATAKTQH NGMNNIIRLF LKKGLVNDLA
RVPHSLDLSS PNMANTVNAA LKPLETLSRI VNQPSSLFGS KSASSKSKSE QDAQGAAQDS
NSNQQDPGEP GEAEVQEEDH DVTQTEVADG DIMDGEAETD SVVIAGQPEV LSSQEMQVEN
ELEDLIDELL ERDGGSGNST IIVSRSGEDE SQEDVLMDEA PSNLSQASTL QANREDSMNI
LDPEDEEEHT QEEDSSGSNE DEDDSQDEEE EEEEDEEDDQ EDDEGEEGDE DDDDDGSEME
LDEDYPDMNA SPLVRFERFD REDDLIIEFD NMFSSATDIP PSPGNIPTTH PLMVRHADHS
SLTLGSGSST TRLTQGIGRS QRTLRQLTAN TGHTIHVHYP GNRQPNPPLI LQRLLGPSAA
ADILQLSSSL PLQSRGRARL LVGNDDVHII ARSDDELLDD FFHDQSTATS QAGTLSSIPT
ALTRWTEECK VLDAESMHDC VSVVKVPIVN HLEFLRDEEL EERREKRRKQ LAEEETKITD
KSKEDKENRD QGAQCTASKT NDSTEQNLSD GTPMPDSYPT TPSSTDAATS ESKDTLVTLQ
PSQQQQTLPP PPALGQIAQE LQSPAGEGGS SAQLLMPVES EELGPTRPSG EAETTQMELS
PAPTITSLSP ERAEDSDALT AVSSQLEGSP MDTSSLASCT LEEAVGDTSA AGSSEQPTAG
SSTPGDAPPV VTEVQGRGDT SGEPTQPPED SSPPASSESS STRDSAVAIS GADSRGILEE
PLPSTSSEEE DPLAGISLPE GVDPSFLAAL PDDIRREVLQ NQLGIRPPTR TAPSANSSAP
AVVGNPGVTE VSPEFLAALP PAIQEEVLAQ QRAEQQRREL AQNASSDTPM DPVTFIQTLP
SDLRRSVLED MEDSVLAVMP PDIAAEAQAL RREQEARQRQ LMHERLFGHS STSALSAILR
SPAFTSRLSG NRGVQYTRLA VQRGGTFQMG GSSSHNRPSG SNVDTLLRLR GRLLLDHEAL
SCLLVLLFVD EPKLNTSRLH RVLRNLCYHA QTRHWVIRSL LSILQRSSES ELCIETPKLS
SSEEKGKKSS KSCGSSSHEN RPLDLLHKME SKSSNQLSWL SVSMDAALGC RTNIFQIQRS
GGRKHTEKHA SSGSTVHIHP QAAPVVCRHV LDTLIQLAKV FPSHFTQQRT KETNCESDRE
RGSNKQACSP CSSQSTSSGI CTDFWDLLVK LDNMNVSRKG KNSVKSVPVS AGGEGETSPY
SLEASPLGQL MNMLSHPVIR RSSLLTEKLL RLLSLISIAL PENKVSETQA NSGSSASSTT
VATSTTSTTT TTAASSTPTP PAATTPVTSA PALVAATAIS TIAVAASTTV TTPTTATTTV
STSTTTKVSK SPAKMGDGGS SSADFKMVSS GLTENQLQLS VEVLTSHSCS EEGLEDAANV
LLQLSRGDPA TRDTVLKLLL NGARHLGYTL CKQIGTLLAE LREYNLEQQR RAQCETLSPD
GLPEEQPQTT KLKGKMQSRF DMAENVVIVA SQKRPLGGRE LQLPSMSMLT SKTSTQKFFL
RVLQVIIQLR DDTRRANKKA KQTGRLGSSG LGSASSIQAA VRQLEAEADA IIQMSESSQS
EASVRREESP MDVDQPSPSA QDTQSIGSDG TPQGEKEKEE RPPELPLLSE QLSLDELWDM
LGECLKELEE SHDQHAVLVL QPAVEAFFLV HATERESKPP IRDTRESQLA HIKDEPPPLS
PAPLTPATPS SLDPFFSREP SSMHISSSLP PDTQKFLRFA ETHRTVLNQI LRQSTTHLAD
GPFAVLVDYI RVLDFDVKRK YFRQELERLD EGLRKEDMAV HVRRDHVFED SYRELHRKSP
EEMKNRLYIV FEGEEGQDAG GLLREWYMII SREMFNPMYA LFRTSPGDRV TYTINPSSHC
NPNHLSYFKF VGRIVAKAVY DNRLLECYFT RSFYKHILGK SVRYTDMESE DYHFYQGLVY
LLENDVSTLG YDLTFSTEVQ EFGVCEVRDL KPNGANILVT EENKKEYVHL VCQMRMTGAI
RKQLAAFLEG FYEIIPKRLI SIFTEQELEL LISGLPTIDI DDLKSNTEYH KYQSNSIQIQ
WFWRALRSFD QADRAKFLQF VTGTSKVPLQ GFAALEGMNG IQKFQIHRDD RSTDRLPSAH
TCFNQLDLPA YESFEKLRHM LLLAIQECSE GFGLA
//
