ID M5C2X4_THACB Unreviewed; 731 AA.
AC M5C2X4;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00012418};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
GN Name=RPO21 {ECO:0000313|EMBL:CCO33694.1};
GN ORFNames=BN14_07779 {ECO:0000313|EMBL:CCO33694.1};
OS Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14) (Lettuce bottom
OS rot fungus) (Rhizoctonia solani).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Cantharellales; Ceratobasidiaceae; Rhizoctonia; Rhizoctonia solani AG-1.
OX NCBI_TaxID=1108050 {ECO:0000313|EMBL:CCO33694.1, ECO:0000313|Proteomes:UP000012065};
RN [1] {ECO:0000313|EMBL:CCO33694.1, ECO:0000313|Proteomes:UP000012065}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AG1-IB / isolate 7/3/14 {ECO:0000313|Proteomes:UP000012065};
RX PubMed=23280342; DOI=10.1016/j.jbiotec.2012.12.010;
RA Wibberg D.W., Jelonek L.J., Rupp O.R., Hennig M.H., Eikmeyer F.E.,
RA Goesmann A.G., Hartmann A.H., Borriss R.B., Grosch R.G., Puehler A.P.,
RA Schlueter A.S.;
RT "Establishment and interpretation of the genome sequence of the
RT phytopathogenic fungus Rhizoctonia solani AG1-IB isolate 7/3/14.";
RL J. Biotechnol. 0:0-0(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCO33694.1}.
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DR EMBL; CAOJ01011921; CCO33694.1; -; Genomic_DNA.
DR AlphaFoldDB; M5C2X4; -.
DR HOGENOM; CLU_411001_0_0_1; -.
DR Proteomes; UP000012065; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:InterPro.
DR CDD; cd02584; RNAP_II_Rpb1_C; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000684; RNA_pol_II_repeat_euk.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR Pfam; PF05001; RNA_pol_Rpb1_R; 9.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR PROSITE; PS00115; RNA_POL_II_REPEAT; 2.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000313|EMBL:CCO33694.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:CCO33694.1}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CCO33694.1};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 1..37
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04992"
FT DOMAIN 38..381
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04998"
FT DOMAIN 122..257
FT /note="RNA polymerase Rpb1"
FT /evidence="ECO:0000259|Pfam:PF04990"
FT REGION 561..642
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 696..731
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 698..731
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 731 AA; 80490 MW; 48C173B1D9F9C42A CRC64;
MHLRSTFATR PVLEELRLTR QAFDWIIGEV ETKFNASIVN PGEMCGTLAA QSIGEPATQM
TLNTFHYAGV SSKNVTLGVP RLKEIINVAT NIKTPSLTVY LQSEYASSKH KAKTIQTELA
HTTLRTITAT TEIIYDPDPT NTIIDEDRDF VETFFAIPDE SVERTLSQQS PWVLRIQLDR
AKVLDKKLDM AYIADRIEQA FYPDMKVIPS DDNSEKLILR CRPIVMQDKD EEAIENTEED
IFIRQIEHNM LDSVTLRGVK GIRRVFMVEH DKPMIDAAGE LQPRSAKEWV LETDGVNLKS
VLSVDGIDFT RTYSNKCPEV FEVLGIEAAR AAILRELRNV IEFDGSYVNY RHLALLTDLM
TNRGTLMAIT RHGINRADTG ALMRCSFEET VEILMEAAAV GEKDDCYGVA ENVLFGQMAP
MGTGSFDVAL DVDMLKDVIV DSRLPIQNMM AAQMGGGATP AGGLAMTPYD SASPMQAEAW
RTEGAAFSPL ATNADEASGG FSFIPYGQSP MGPDSRFGGY SPSSPGYSPT SPFMPTSPMV
TATSPYGASP FGATSPYATS PAYSPTSPNM NLTSPQYSPT SPQYSPTSPT YSPTSPTYSP
TSPRYYSTSP SFSPASPRYS PTSPRYSPTS PMQASPTSPK MTSPRYCEFK SMRWTSANSG
FLQLQRRQRI APHLQLILQC HRLTVLPLLF GHHDATPAPG DTNNQNQQNQ TNGQTSPWGN
TATYQASPSW K
//
