ID M5CE93_THACB Unreviewed; 490 AA.
AC M5CE93;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Extracellular metalloproteinase {ECO:0000256|RuleBase:RU364017};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU364017};
DE AltName: Full=Fungalysin {ECO:0000256|RuleBase:RU364017};
GN ORFNames=BN14_11656 {ECO:0000313|EMBL:CCO37500.1};
OS Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14) (Lettuce bottom
OS rot fungus) (Rhizoctonia solani).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Cantharellales; Ceratobasidiaceae; Rhizoctonia; Rhizoctonia solani AG-1.
OX NCBI_TaxID=1108050 {ECO:0000313|EMBL:CCO37500.1, ECO:0000313|Proteomes:UP000012065};
RN [1] {ECO:0000313|EMBL:CCO37500.1, ECO:0000313|Proteomes:UP000012065}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AG1-IB / isolate 7/3/14 {ECO:0000313|Proteomes:UP000012065};
RX PubMed=23280342; DOI=10.1016/j.jbiotec.2012.12.010;
RA Wibberg D.W., Jelonek L.J., Rupp O.R., Hennig M.H., Eikmeyer F.E.,
RA Goesmann A.G., Hartmann A.H., Borriss R.B., Grosch R.G., Puehler A.P.,
RA Schlueter A.S.;
RT "Establishment and interpretation of the genome sequence of the
RT phytopathogenic fungus Rhizoctonia solani AG1-IB isolate 7/3/14.";
RL J. Biotechnol. 0:0-0(2013).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR601842-2};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR601842-2};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU364017}.
CC -!- SIMILARITY: Belongs to the peptidase M36 family.
CC {ECO:0000256|RuleBase:RU364017}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCO37500.1}.
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DR EMBL; CAOJ01017210; CCO37500.1; -; Genomic_DNA.
DR AlphaFoldDB; M5CE93; -.
DR HOGENOM; CLU_012703_4_2_1; -.
DR Proteomes; UP000012065; Unassembled WGS sequence.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.10.170.10; -; 1.
DR InterPro; IPR025711; PepSY.
DR InterPro; IPR001842; Peptidase_M36.
DR PANTHER; PTHR33478; EXTRACELLULAR METALLOPROTEINASE MEP; 1.
DR PANTHER; PTHR33478:SF1; EXTRACELLULAR METALLOPROTEINASE MEP; 1.
DR Pfam; PF03413; PepSY; 1.
DR Pfam; PF02128; Peptidase_M36; 2.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 2.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU364017, ECO:0000313|EMBL:CCO37500.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601842-2,
KW ECO:0000256|RuleBase:RU364017};
KW Metalloprotease {ECO:0000256|RuleBase:RU364017};
KW Protease {ECO:0000256|RuleBase:RU364017};
KW Secreted {ECO:0000256|RuleBase:RU364017};
KW Signal {ECO:0000256|RuleBase:RU364017};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR601842-2};
KW Zymogen {ECO:0000256|RuleBase:RU364017}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|RuleBase:RU364017"
FT CHAIN 21..490
FT /note="Extracellular metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU364017"
FT /id="PRO_5009368178"
FT DOMAIN 152..217
FT /note="PepSY"
FT /evidence="ECO:0000259|Pfam:PF03413"
FT BINDING 223
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR601842-2"
SQ SEQUENCE 490 AA; 52977 MW; F9D9760EA978E11A CRC64;
MVLITRFAIL AFLTAQCVLA APWKEPRCWT THSVRSIGPT GSQFKSYHPK PIFESYGVDG
IVHPLAKRDI SGTNEEAAKA FLEEKFGIEV QELSHKSGHS STNVANEYFK QVINNITVAN
AVANVALKGQ KVVSFGASFV KPKRVAAATP ELTEEDAISK AEDAIGGKYN TLPTSVEYFA
KDSGDVVLTR VVQVQNDDTD EWYEVFVDAN SGEIVNVISF VAHAKYRVIP FALQNPNDGF
QVLSDPQDPI TSPDGWHRYR LNFSNTTVIT NATSGNNAHV YKSSVAEGLT GQNSAPDVNV
YEHTFDPSKE PSGNKDAASV NVFYLANMIH DLLYHYGFIE SSYNFQYYNY GKGGSGNDPV
YISVQDGGKT NFNNANFATP PDGANAGEVW ANIGHEIYAA LVAKSGLSTD KKSVSSTAGN
VVALHLLIDA LATQPCNPTF ISARDAIIQA DANRYGSANK CTLWNAFAKR GLGLGATMTK
VDSKTLPSGC
//
