ID M7SVW0_EUTLA Unreviewed; 646 AA.
AC M7SVW0;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=3-hydroxyisobutyrate dehydrogenase {ECO:0000256|ARBA:ARBA00012991};
DE EC=1.1.1.31 {ECO:0000256|ARBA:ARBA00012991};
GN ORFNames=UCREL1_4352 {ECO:0000313|EMBL:EMR68633.1};
OS Eutypa lata (strain UCR-EL1) (Grapevine dieback disease fungus) (Eutypa
OS armeniacae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Xylariomycetidae; Xylariales; Diatrypaceae; Eutypa.
OX NCBI_TaxID=1287681 {ECO:0000313|EMBL:EMR68633.1, ECO:0000313|Proteomes:UP000012174};
RN [1] {ECO:0000313|Proteomes:UP000012174}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCR-EL1 {ECO:0000313|Proteomes:UP000012174};
RX PubMed=23723393; DOI=10.1128/genomeA.00228-13;
RA Blanco-Ulate B., Rolshausen P.E., Cantu D.;
RT "Draft genome sequence of the grapevine dieback fungus Eutypa lata UCR-
RT EL1.";
RL Genome Announc. 1:E0022813-E0022813(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-
CC oxopropanoate + H(+) + NADH; Xref=Rhea:RHEA:17681, ChEBI:CHEBI:11805,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57700,
CC ChEBI:CHEBI:57945; EC=1.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00000062};
CC -!- PATHWAY: Amino-acid degradation; L-valine degradation.
CC {ECO:0000256|ARBA:ARBA00005109}.
CC -!- SIMILARITY: Belongs to the HIBADH-related family. 3-hydroxyisobutyrate
CC dehydrogenase subfamily. {ECO:0000256|ARBA:ARBA00006013}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KB706202; EMR68633.1; -; Genomic_DNA.
DR RefSeq; XP_007792274.1; XM_007794083.1.
DR AlphaFoldDB; M7SVW0; -.
DR STRING; 1287681.M7SVW0; -.
DR KEGG; ela:UCREL1_4352; -.
DR eggNOG; KOG0409; Eukaryota.
DR HOGENOM; CLU_443534_0_0_1; -.
DR OMA; CWADELG; -.
DR OrthoDB; 203032at2759; -.
DR Proteomes; UP000012174; Unassembled WGS sequence.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:UniProt.
DR GO; GO:0009083; P:branched-chain amino acid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd07730; metallo-hydrolase-like_MBL-fold; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.60.15.10; Ribonuclease Z/Hydroxyacylglutathione hydrolase-like; 1.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR006115; 6PGDH_NADP-bd.
DR InterPro; IPR029154; HIBADH-like_NADP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR PANTHER; PTHR22981:SF7; 3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR22981; 3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED; 1.
DR Pfam; PF14833; NAD_binding_11; 1.
DR Pfam; PF03446; NAD_binding_2; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF56281; Metallo-hydrolase/oxidoreductase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Branched-chain amino acid catabolism {ECO:0000256|ARBA:ARBA00022456};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000012174}.
FT DOMAIN 317..495
FT /note="6-phosphogluconate dehydrogenase NADP-binding"
FT /evidence="ECO:0000259|Pfam:PF03446"
FT DOMAIN 512..638
FT /note="3-hydroxyisobutyrate dehydrogenase-like NAD-binding"
FT /evidence="ECO:0000259|Pfam:PF14833"
FT REGION 199..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 646 AA; 68060 MW; 53B623FBFA69DD51 CRC64;
MTATTDPKPP VFVTVSALDA GHLTLPERLF ITDADPEKSN TVPSLSFLIQ HPSPSPSREG
SQTTNLVFDL GIKRDLDRYT PSQQIHTAQR QPVITDPDCA ASLRYGVPGK GKPKPKKDEV
LLDPSKDIDL VIISHVHWDH VGTPSDFSSA TFAVGSGTLD LLRNGAGHLY PADLFDEDEL
PRTRTVEFPV VPGATEYADG SDIPTHTATP ENSEAKLPPS ASQWSWGRLG GFPNALDLFG
DGSVYVIDSP GHLYGHVNLL TRISEAKYVY LGGDCCHDTR ILSGKSDIAM YGDGKGGLRS
VHVDTNAAKK TLDRIRAFVS LGVMGYPMAK NLRVGLGPET TLLICDVNTE AIARFQKETE
GQGPVSVVTN GFEAVKAANT VITMLPGSAA VKAVYLDPGT GVLAGAVAAA AENGSGNALL
PPKLIMECGT IETDTITSVA EAAQATSAAK LHSTLTFVDA PVSGGPMGAQ AGTLTFMVGC
QPAASAQIFP VVKSYLRHMG NADGIFLCGD VGAGTAFKII NNYLSAITSL AASEALNIGV
KAGLDPKLLT DVINGSGGQC WVTSKSNPVP GVQDNVPSSR GYEGGFRIEL CAKVLGMGSR
LAETVGARTV LDRPTLDAFG EAIADERYAG KDARVVYKWL NDSERK
//
