ID M9P8F1_9CHLO Unreviewed; 561 AA.
AC M9P8F1;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Light-independent protochlorophyllide reductase subunit N {ECO:0000256|HAMAP-Rule:MF_00352};
DE Short=DPOR subunit N {ECO:0000256|HAMAP-Rule:MF_00352};
DE Short=LI-POR subunit N {ECO:0000256|HAMAP-Rule:MF_00352};
DE EC=1.3.7.7 {ECO:0000256|HAMAP-Rule:MF_00352};
GN Name=chlN {ECO:0000313|EMBL:AFY64458.1};
OS Pleodorina starrii.
OG Plastid {ECO:0000313|EMBL:AFY64458.1}.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Volvocaceae; Pleodorina.
OX NCBI_TaxID=330485 {ECO:0000313|EMBL:AFY64458.1};
RN [1] {ECO:0000313|EMBL:AFY64458.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NIES-1363 {ECO:0000313|EMBL:AFY64458.1};
RX PubMed=23300255; DOI=10.1093/molbev/mst002;
RA Smith D.R., Hamaji T., Olson B.J., Durand P.M., Ferris P., Michod R.E.,
RA Featherston J., Nozaki H., Keeling P.J.;
RT "Organelle genome complexity scales positively with organism size in
RT volvocine green algae.";
RL Mol. Biol. Evol. 30:793-797(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 ADP + chlorophyllide a + oxidized 2[4Fe-4S]-[ferredoxin] + 2
CC phosphate = 2 ATP + 2 H2O + protochlorophyllide a + reduced 2[4Fe-
CC 4S]-[ferredoxin]; Xref=Rhea:RHEA:28202, Rhea:RHEA-COMP:10002,
CC Rhea:RHEA-COMP:10004, ChEBI:CHEBI:15377, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33722, ChEBI:CHEBI:33723, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83348, ChEBI:CHEBI:83350, ChEBI:CHEBI:456216; EC=1.3.7.7;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00352};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00352};
CC Note=Binds 1 [4Fe-4S] cluster per heterodimer. The cluster is bound at
CC the heterodimer interface by residues from both subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00352};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
CC -!- SIMILARITY: Belongs to the BchN/ChlN family. {ECO:0000256|HAMAP-
CC Rule:MF_00352}.
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DR EMBL; JX977846; AFY64458.1; -; Genomic_DNA.
DR RefSeq; YP_007890189.1; NC_021109.1.
DR AlphaFoldDB; M9P8F1; -.
DR GeneID; 15332319; -.
DR UniPathway; UPA00670; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016730; F:oxidoreductase activity, acting on iron-sulfur proteins as donors; IEA:InterPro.
DR GO; GO:0016636; F:oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor; IEA:UniProtKB-UniRule.
DR GO; GO:0036068; P:light-independent chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019685; P:photosynthesis, dark reaction; IEA:InterPro.
DR CDD; cd01979; Pchlide_reductase_N; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 3.
DR HAMAP; MF_00352; ChlN_BchN; 1.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR005970; Protochl_reductN.
DR NCBIfam; TIGR01279; DPOR_bchN; 1.
DR PANTHER; PTHR39429; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT N; 1.
DR PANTHER; PTHR39429:SF3; LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE SUBUNIT N; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|HAMAP-Rule:MF_00352};
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_00352};
KW Chlorophyll biosynthesis {ECO:0000256|ARBA:ARBA00023171, ECO:0000256|HAMAP-
KW Rule:MF_00352}; Iron {ECO:0000256|HAMAP-Rule:MF_00352};
KW Iron-sulfur {ECO:0000256|HAMAP-Rule:MF_00352};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00352};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00352};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, ECO:0000256|HAMAP-
KW Rule:MF_00352};
KW Photosynthesis {ECO:0000256|ARBA:ARBA00022531, ECO:0000256|HAMAP-
KW Rule:MF_00352}; Plastid {ECO:0000313|EMBL:AFY64458.1}.
FT DOMAIN 94..537
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
FT BINDING 94
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared with heterodimeric partner"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00352"
FT BINDING 119
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared with heterodimeric partner"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00352"
FT BINDING 179
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="ligand shared with heterodimeric partner"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00352"
SQ SEQUENCE 561 AA; 63446 MW; 31069A536EB54903 CRC64;
MFTLQSKVTR EVKSLINKKN KNEIPLDCYT QLVYQTLLLT PRVHNNLEHL KNKKQRYNTI
LSNFVNSAGD SSLYNLLFLN GLFECETGNY HTFCPISCVA WLYQKIEDSF FLVIGTKTCG
YFLQNALGVM IFAEPRYAMA ELEESDISAQ LNDYKELKRL CLHIKQDRNP SVIVWIGTCT
TEIIKMDLEG MAPRLEADIG LPIVVARANG LDYAFTQGED TVLSAMALAS LKQKTVPKIT
SPVDTALHKI DVPQADQKNR QLNQRSIIPD TEKKNKANIH SLVLFGSVPS TVATQLTLEL
KKEGITVSGW LPSQRYHDLP NFNKNTFVCG INPFLSRTAT TLMRRSKCTL ICAPFPIGPD
GTRVWIEKIC GAFGICPSVN HSTALVTETK TKLQEREKFI FEGLEDYLKF IRGKSVFFMG
DNLLEISLAR FLTRCGMIVY EIGIPYLDKR FQAAELALLE QTCKEMNVPM PRIVEKPDNY
YQIQRIRELQ PDLTITGMAH ANPLEARGIT TKWSVEFTFA QIHGFTNTRE ILELVTRPLR
RNLMCSLTTK AKGNKTASLA A
//
