ID N1MSW4_9SPHN Unreviewed; 450 AA.
AC N1MSW4;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=site-specific DNA-methyltransferase (cytosine-N(4)-specific) {ECO:0000256|ARBA:ARBA00012185};
DE EC=2.1.1.113 {ECO:0000256|ARBA:ARBA00012185};
GN ORFNames=EBBID32_42040 {ECO:0000313|EMBL:CCW19834.1};
OS Sphingobium indicum BiD32.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=1301087 {ECO:0000313|EMBL:CCW19834.1, ECO:0000313|Proteomes:UP000013201};
RN [1] {ECO:0000313|EMBL:CCW19834.1, ECO:0000313|Proteomes:UP000013201}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BiD32 {ECO:0000313|EMBL:CCW19834.1,
RC ECO:0000313|Proteomes:UP000013201};
RA Le V.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CCW19834.1, ECO:0000313|Proteomes:UP000013201}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BiD32 {ECO:0000313|EMBL:CCW19834.1,
RC ECO:0000313|Proteomes:UP000013201};
RA Nielsen J.L., Zhou N.A., Kjeldal H.;
RT "Bisphenol A degrading Sphingobium sp. strain BiD32.";
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC Evidence={ECO:0000256|ARBA:ARBA00001893};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC subfamily. {ECO:0000256|ARBA:ARBA00010203}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCW19834.1}.
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DR EMBL; CAVK010000233; CCW19834.1; -; Genomic_DNA.
DR RefSeq; WP_006966172.1; NZ_CAVK010000233.1.
DR AlphaFoldDB; N1MSW4; -.
DR OrthoDB; 9800801at2; -.
DR Proteomes; UP000013201; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR017985; MeTrfase_CN4_CS.
DR InterPro; IPR000241; RlmKL-like_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF01170; UPF0020; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00093; N4_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000013201};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 76..102
FT /note="UPF0020"
FT /evidence="ECO:0000259|Pfam:PF01170"
SQ SEQUENCE 450 AA; 49240 MW; E830DDD451BEA630 CRC64;
MSMSVTQQKR NDAAESSLRD LFARFESDGS AVEVNFRQLL SSDGGTSKSA HGIHPYPAKL
LVNIPQFFLA AIPTVAGSAL LDPFCGSGTV LYEGALAGLR PIGSDSNPLA RLITQAKLTP
IDREVARRQL DEVMAAYPLV SPTIPDVVNR DYWFSERISD CLGRLKTAIE CSVSAQHRPV
FDVSFSATVR RVSYADPRLS VPVKINPDRS ARYGAKGDEV IRRLGRLAKI DVGAVFWSIT
NQNIQRCLEL GQGFVLDQPE IFSDARSLGL ADASVGLAIT SPPYVGAQKY VRASSLSLGW
LGLTPDCKLR PIEQQSIGRE HLTAKEELAP SGTGLAEADC LLDEIVERNP LRARIAQRYL
LEMRDALSEL SRTMRHDGRL ILVVGPNLVA GLEFPTPLFL QKIAESFGFS TQLHLIDSID
SRGLMTKRNR TAGLIQQESV FLLKRVRAGA
//
