ID N9L5E8_9GAMM Unreviewed; 524 AA.
AC N9L5E8;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=F905_00054 {ECO:0000313|EMBL:ENW91523.1};
OS Acinetobacter sp. CIP 53.82.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Acinetobacter.
OX NCBI_TaxID=1144671 {ECO:0000313|EMBL:ENW91523.1, ECO:0000313|Proteomes:UP000018455};
RN [1] {ECO:0000313|EMBL:ENW91523.1, ECO:0000313|Proteomes:UP000018455}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CIP 53.82 {ECO:0000313|EMBL:ENW91523.1,
RC ECO:0000313|Proteomes:UP000018455};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Cerqueira G., Feldgarden M., Courvalin P., Perichon B.,
RA Grillot-Courvalin C., Clermont D., Rocha E., Yoon E.-J., Nemec A.,
RA Walker B., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B.,
RA Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., McCowan C., Murphy C., Neiman D., Pearson M.,
RA Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Acinetobacter sp. CIP 53.82.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ENW91523.1}.
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DR EMBL; APRK01000006; ENW91523.1; -; Genomic_DNA.
DR RefSeq; WP_005175515.1; NZ_KB850044.1.
DR AlphaFoldDB; N9L5E8; -.
DR PATRIC; fig|1144671.3.peg.40; -.
DR HOGENOM; CLU_013049_0_2_6; -.
DR Proteomes; UP000018455; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..167
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 178..481
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 486..513
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 524 AA; 59619 MW; 4777DCCBCB9221C6 CRC64;
MTQVQLQQLQ KHLWNIANDL RGKMGADEFR DYILGFIFYK YLSEKAISFA NELLKDEGLA
EKYYQLDENN PDHAELIDGI KENSVEEVGY ALAPKQLFHT IAERGRQGEF ILDELSNTLR
EIERSTLGAE SEDDFANLFE DLDLNSTKLG NNANDRNELV SKVLTHLDNI DFDLSNSESD
VLGDAYEYLI GEFASGAGKK AGEFYTPQTV STLLAKIVTQ GKERLRSVYD PTCGSGSLLL
RVKREVQDVD MIYGQEMNRT TYNLARMNMI LHDVHFAKFD IKQEDTLTRP QHKDLRFEAV
VANPPFSAKW SADPIFLQDE RFSRFGKLAP SSKADMAFVQ HMLHQLDHNG TMAVVLPHGV
LFRGSSEGVI RQYMIEKLNV IDAVIGLPAN IFYGTTIPTC ILVLKKNREH KDNILFIDAS
NEFEKQKNQN KLLPEHLDNI IGAFENRQNI DKYAHVATLQ EVKDNDYNLN IPRYVDTFEA
EGDIDLNAIA QQLKELEEQS EKTDKVIAEF CKELGIDSPF SEVK
//
