ID NELFD_PIG Reviewed; 590 AA.
AC A5GFY4; A5GFY3;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE RecName: Full=Negative elongation factor D;
DE Short=NELF-D;
DE AltName: Full=TH1-like protein;
GN Name=NELFCD; Synonyms=NELFD, TH1L;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Porcine genome sequencing project;
RL Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential component of the NELF complex, a complex that
CC negatively regulates the elongation of transcription by RNA polymerase
CC II (By similarity). The NELF complex, which acts via an association
CC with the DSIF complex and causes transcriptional pausing, is
CC counteracted by the P-TEFb kinase complex (By similarity).
CC {ECO:0000250|UniProtKB:Q8IXH7}.
CC -!- SUBUNIT: The NELF complex is composed of NELFA, NELFB, NELFCD and
CC NELFE; NELFA and NELFCD form a stable subcomplex that binds primarily
CC through NELFCD to the N-terminus of NELFB (By similarity). Binds RNA
CC which may help to stabilize the NELF complex on nucleic acid (By
CC similarity). In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and
CC ssRNA in a sequence- and structure-dependent manner (By similarity).
CC Interacts with ARAF1 (By similarity). Interacts with PCF11 (By
CC similarity). Interacts with NELFB (By similarity). Interacts with KAT8
CC (By similarity). {ECO:0000250|UniProtKB:Q8IXH7,
CC ECO:0000250|UniProtKB:Q922L6}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8IXH7}.
CC -!- SIMILARITY: Belongs to the NELF-D family. {ECO:0000305}.
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DR EMBL; CR956646; CAN13211.1; -; Genomic_DNA.
DR EMBL; CR956646; CAN13212.1; -; Genomic_DNA.
DR RefSeq; NP_001107170.1; NM_001113698.1.
DR RefSeq; XP_005673120.1; XM_005673063.2.
DR AlphaFoldDB; A5GFY4; -.
DR SMR; A5GFY4; -.
DR STRING; 9823.ENSSSCP00000033095; -.
DR PaxDb; 9823-ENSSSCP00000008029; -.
DR PeptideAtlas; A5GFY4; -.
DR Ensembl; ENSSSCT00060053771.1; ENSSSCP00060022918.1; ENSSSCG00060039723.1.
DR Ensembl; ENSSSCT00060053840.1; ENSSSCP00060022956.1; ENSSSCG00060039723.1.
DR GeneID; 100134985; -.
DR KEGG; ssc:100134985; -.
DR CTD; 51497; -.
DR eggNOG; ENOG502QPUE; Eukaryota.
DR HOGENOM; CLU_028060_0_0_1; -.
DR InParanoid; A5GFY4; -.
DR OrthoDB; 306548at2759; -.
DR TreeFam; TF324118; -.
DR Reactome; R-SSC-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-SSC-113418; Formation of the Early Elongation Complex.
DR Reactome; R-SSC-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SSC-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-SSC-75955; RNA Polymerase II Transcription Elongation.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR Proteomes; UP000694570; Unplaced.
DR Proteomes; UP000694571; Unplaced.
DR Proteomes; UP000694720; Unplaced.
DR Proteomes; UP000694722; Unplaced.
DR Proteomes; UP000694723; Unplaced.
DR Proteomes; UP000694724; Unplaced.
DR Proteomes; UP000694725; Unplaced.
DR Proteomes; UP000694726; Unplaced.
DR Proteomes; UP000694727; Unplaced.
DR Proteomes; UP000694728; Unplaced.
DR Genevisible; A5GFY4; SS.
DR GO; GO:0032021; C:NELF complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0034244; P:negative regulation of transcription elongation by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR006942; TH1.
DR PANTHER; PTHR12144:SF0; NEGATIVE ELONGATION FACTOR C_D; 1.
DR PANTHER; PTHR12144; NEGATIVE ELONGATION FACTOR D; 1.
DR Pfam; PF04858; TH1; 1.
PE 3: Inferred from homology;
KW Nucleus; Reference proteome; Repressor; RNA-binding; Transcription;
KW Transcription regulation.
FT CHAIN 1..590
FT /note="Negative elongation factor D"
FT /id="PRO_0000312639"
FT REGION 15..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..41
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 28
FT /note="G -> GQ (in Ref. 1; CAN13211)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 590 AA; 66218 MW; 9CDA21F259C0EC65 CRC64;
MAGAAPGAIM DEDYFGSAAE WGDEADGGQQ EDDYGEGEDD AEVQQECLHK FSTRDYIMEP
SIFNTLKRYF QAGGSPENVI QLLSENYTAV AQTVNLLAEW LIQTGVEPVQ VQETVENHLK
SLLIKHFDPR KADSIFTEEG ETPAWLEQMI AHTTWRDLFY KLAEAHPDCL MLNFTVKLIS
DAGYQGEITS VSTACQQLEV FSRVLRTSLA TILDGGEENL EKHLPEFAKM VCHGEHTYLF
AQAMMSVLAQ EEQGGSAVRR VAQEVQRFAQ EKGHDASQIT LALGTAASYP RACQALGAML
SKGALNPADI TVLFKMFTSM DPPPVELIRV PAFLDLFMQS LFKPGARINQ DHKHKYIHIL
AYAASVVETW KKNKRVSINK DELKSTSKAV ETVHNLCCNE NKGASELVAE LSTLYQCIRF
PVVAMGVLKW VDWTVSEPRY FQLQTDHTPV HLALLDEIST CHQLLHPQVL QLLVKLFETE
HSQLDVMEQL ELKKTLLDRM VHLLSRGHVL PVVSYIRKCL EKLDTDISLI RHFVTEVLDV
IAPPYTSDFV QLFLPILEND SIAGTIKTEG EHDPVTEFIA HCKSNFILVN
//
