ID NIRD_PSEAE Reviewed; 150 AA.
AC P95412; Q7DCL2;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 27-MAR-2024, entry version 118.
DE RecName: Full=Siroheme decarboxylase NirD subunit {ECO:0000305};
DE EC=4.1.1.111 {ECO:0000250|UniProtKB:I6UH61};
GN Name=nirD {ECO:0000303|PubMed:8982003}; OrderedLocusNames=PA0515;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8982003; DOI=10.1128/jb.179.1.235-242.1997;
RA Kawasaki S., Arai H., Kodama T., Igarashi Y.;
RT "Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas
RT aeruginosa: sequencing and identification of a locus for heme d1
RT biosynthesis.";
RL J. Bacteriol. 179:235-242(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Involved in heme d1 biosynthesis (PubMed:8982003). Catalyzes
CC the decarboxylation of siroheme into didecarboxysiroheme (By
CC similarity). {ECO:0000250|UniProtKB:I6UH61,
CC ECO:0000269|PubMed:8982003}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + siroheme = 12,18-didecarboxysiroheme + 2 CO2;
CC Xref=Rhea:RHEA:19093, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:60052, ChEBI:CHEBI:140497; EC=4.1.1.111;
CC Evidence={ECO:0000250|UniProtKB:I6UH61};
CC -!- PATHWAY: Porphyrin-containing compound metabolism.
CC {ECO:0000305|PubMed:8982003}.
CC -!- SUBUNIT: Probably forms a complex composed of NirD, NirL, NirG and
CC NirH. All proteins are required for the total conversion of siroheme to
CC didecarboxysiroheme. {ECO:0000250|UniProtKB:I6UH61}.
CC -!- DISRUPTION PHENOTYPE: The nirDLGH mutant lacks dissimilatory nitrite
CC reductase (NIR) activity. The NIR activity is restored by adding
CC purified heme d1. {ECO:0000269|PubMed:8982003}.
CC -!- SIMILARITY: Belongs to the Ahb/Nir family. {ECO:0000305}.
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DR EMBL; D84475; BAA12677.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG03904.1; -; Genomic_DNA.
DR PIR; G83581; G83581.
DR PIR; PC4131; PC4131.
DR RefSeq; NP_249206.1; NC_002516.2.
DR RefSeq; WP_003084864.1; NZ_QZGE01000010.1.
DR AlphaFoldDB; P95412; -.
DR SMR; P95412; -.
DR STRING; 208964.PA0515; -.
DR PaxDb; 208964-PA0515; -.
DR DNASU; 879538; -.
DR GeneID; 879538; -.
DR KEGG; pae:PA0515; -.
DR PATRIC; fig|208964.12.peg.545; -.
DR PseudoCAP; PA0515; -.
DR HOGENOM; CLU_112007_1_0_6; -.
DR InParanoid; P95412; -.
DR OrthoDB; 9806536at2; -.
DR PhylomeDB; P95412; -.
DR BioCyc; PAER208964:G1FZ6-520-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR Gene3D; 3.30.70.3460; -; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR000485; AsnC-type_HTH_dom.
DR InterPro; IPR040523; AsnC_trans_reg2.
DR InterPro; IPR019888; Tscrpt_reg_AsnC-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR43413:SF1; SIROHEME DECARBOXYLASE NIRL SUBUNIT; 1.
DR PANTHER; PTHR43413; TRANSCRIPTIONAL REGULATOR, ASNC FAMILY; 1.
DR Pfam; PF17805; AsnC_trans_reg2; 1.
DR Pfam; PF13404; HTH_AsnC-type; 1.
DR SMART; SM00344; HTH_ASNC; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome.
FT CHAIN 1..150
FT /note="Siroheme decarboxylase NirD subunit"
FT /id="PRO_0000287793"
SQ SEQUENCE 150 AA; 17017 MW; EF158C336FB47602 CRC64;
MDDLSRRLLA RYQKGLPICA EPYRRMAETL GCSEAEVLER LRRLEADGAL SRVGPVLRHQ
RAGASTLAAL AVPEERLQRV AERISQYAEV NHNYQREHRY NLWFVLTAGD RAQLDRVLAE
IAADTGLQPL DLPMQEAYCI DLAFPLEASR
//
