ID NRPD7_ARATH Reviewed; 174 AA.
AC Q8LE42; Q9LIK8;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 27-MAR-2024, entry version 124.
DE RecName: Full=DNA-directed RNA polymerase IV subunit 7;
GN Name=NRPD7; OrderedLocusNames=At3g22900;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=19110459; DOI=10.1016/j.molcel.2008.12.015;
RA Ream T.S., Haag J.R., Wierzbicki A.T., Nicora C.D., Norbeck A.D., Zhu J.K.,
RA Hagen G., Guilfoyle T.J., Pasa-Tolic L., Pikaard C.S.;
RT "Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal
RT their origins as specialized forms of RNA polymerase II.";
RL Mol. Cell 33:192-203(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH NRPD1, AND SUBUNIT.
RX PubMed=21811420; DOI=10.1371/journal.pgen.1002195;
RA Law J.A., Vashisht A.A., Wohlschlegel J.A., Jacobsen S.E.;
RT "SHH1, a homeodomain protein required for DNA methylation, as well as RDR2,
RT RDM4, and chromatin remodeling factors, associate with RNA polymerase IV.";
RL PLoS Genet. 7:E1002195-E1002195(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Component of RNA polymerase IV which mediates 24-nt short-interfering
CC RNAs (siRNA) accumulation. Implicated in siRNA-directed heterochromatin
CC formation through the action of DCL3 and AGO4, and subsequent DNA
CC methylation-dependent silencing of targeted sequences. Essential
CC component of a self-reinforcing loop coupling de novo DNA methylation
CC to siRNA production. Required for intercellular but not intracellular
CC RNA interference (RNAi) leading to systemic post-transcriptional gene
CC silencing. Involved in the maintenance of post-transcriptional RNA
CC silencing. {ECO:0000269|PubMed:19110459}.
CC -!- SUBUNIT: Component of the RNA polymerase IV complex. Interacts with
CC NRPD1. {ECO:0000269|PubMed:19110459, ECO:0000269|PubMed:21811420}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB03035.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AP001300; BAB03035.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE76689.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64801.1; -; Genomic_DNA.
DR EMBL; AY085637; AAM62858.1; -; mRNA.
DR RefSeq; NP_001326806.1; NM_001338603.1.
DR RefSeq; NP_566719.1; NM_113190.3.
DR AlphaFoldDB; Q8LE42; -.
DR SMR; Q8LE42; -.
DR BioGRID; 7194; 1.
DR STRING; 3702.Q8LE42; -.
DR PaxDb; 3702-AT3G22900-1; -.
DR ProteomicsDB; 250479; -.
DR EnsemblPlants; AT3G22900.1; AT3G22900.1; AT3G22900.
DR EnsemblPlants; AT3G22900.2; AT3G22900.2; AT3G22900.
DR GeneID; 821862; -.
DR Gramene; AT3G22900.1; AT3G22900.1; AT3G22900.
DR Gramene; AT3G22900.2; AT3G22900.2; AT3G22900.
DR KEGG; ath:AT3G22900; -.
DR Araport; AT3G22900; -.
DR TAIR; AT3G22900; NRPD7.
DR eggNOG; KOG3298; Eukaryota.
DR HOGENOM; CLU_085878_3_0_1; -.
DR InParanoid; Q8LE42; -.
DR OMA; NIFMSAK; -.
DR OrthoDB; 644205at2759; -.
DR PhylomeDB; Q8LE42; -.
DR PRO; PR:Q8LE42; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LE42; baseline and differential.
DR Genevisible; Q8LE42; AT.
DR GO; GO:0000418; C:RNA polymerase IV complex; IDA:UniProtKB.
DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:InterPro.
DR CDD; cd04329; RNAP_II_Rpb7_N; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.30.1490.120; RNA polymerase Rpb7-like, N-terminal domain; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR InterPro; IPR045113; Rpb7-like.
DR InterPro; IPR005576; Rpb7-like_N.
DR PANTHER; PTHR12709; DNA-DIRECTED RNA POLYMERASE II, III; 1.
DR PANTHER; PTHR12709:SF7; DNA-DIRECTED RNA POLYMERASE IV SUBUNIT 7; 1.
DR Pfam; PF03876; SHS2_Rpb7-N; 1.
DR SUPFAM; SSF88798; N-terminal, heterodimerisation domain of RBP7 (RpoE); 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
PE 1: Evidence at protein level;
KW DNA-directed RNA polymerase; Nucleus; Reference proteome; Transcription.
FT CHAIN 1..174
FT /note="DNA-directed RNA polymerase IV subunit 7"
FT /id="PRO_0000423333"
SQ SEQUENCE 174 AA; 19849 MW; F9B1D02669771280 CRC64;
MFIKVKLPWD VTIPAEDMDT GLMLQRAIVI RLLEAFSKEK ATKDLGYLIT PTILENIGEG
KIKEQTGEIQ FPVVFNGICF KMFKGEIVHG VVHKVHKTGV FLKSGPYEII YLSHMKMPGY
EFIPGENPFF MNQYMSRIQI GARVRFVVLD TEWREAEKDF MALASIDGDN LGPF
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