ID NUC2_NEUCR Reviewed; 1066 AA.
AC Q01317; A7UXH8; Q7RW36;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 09-MAY-2003, sequence version 2.
DT 27-MAR-2024, entry version 134.
DE RecName: Full=Ankyrin repeat protein nuc-2;
DE AltName: Full=Nuclease 2;
GN Name=nuc-2; ORFNames=B23G1.010, NCU01847, NCU11426;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=74-OR23-1VA / FGSC 2489;
RX PubMed=8917314; DOI=10.1007/bf02173977;
RA Poleg Y., Aramayo R., Kang S., Hall J.G., Metzenberg R.L.;
RT "NUC-2, a component of the phosphate-regulated signal transduction pathway
RT in Neurospora crassa, is an ankyrin repeat protein.";
RL Mol. Gen. Genet. 252:709-716(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Controls phosphorus acquisition.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDO64869.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U51118; AAB03277.1; -; Genomic_DNA.
DR EMBL; BX284754; CAD70463.1; -; Genomic_DNA.
DR EMBL; CM002237; EDO64869.2; ALT_SEQ; Genomic_DNA.
DR PIR; S72479; S72479.
DR RefSeq; XP_001727960.2; XM_001727908.2.
DR AlphaFoldDB; Q01317; -.
DR SMR; Q01317; -.
DR STRING; 367110.Q01317; -.
DR PaxDb; 5141-EFNCRP00000009831; -.
DR EnsemblFungi; EDO64869; EDO64869; NCU11426.
DR GeneID; 5846981; -.
DR KEGG; ncr:NCU11426; -.
DR HOGENOM; CLU_002842_1_0_1; -.
DR InParanoid; Q01317; -.
DR OrthoDB; 4907at2759; -.
DR Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR CDD; cd08578; GDPD_NUC-2_fungi; 1.
DR CDD; cd14483; SPX_PHO81_NUC-2_like; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 3.20.20.190; Phosphatidylinositol (PI) phosphodiesterase; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR030395; GP_PDE_dom.
DR InterPro; IPR001995; Peptidase_A2_cat.
DR InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR InterPro; IPR004331; SPX_dom.
DR PANTHER; PTHR24198; ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR24198:SF165; IPT_TIG DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF12796; Ank_2; 3.
DR Pfam; PF03009; GDPD; 1.
DR Pfam; PF03105; SPX; 2.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 9.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF51695; PLC-like phosphodiesterases; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 4.
DR PROSITE; PS51704; GP_PDE; 1.
DR PROSITE; PS51382; SPX; 1.
PE 4: Predicted;
KW ANK repeat; Reference proteome; Repeat.
FT CHAIN 1..1066
FT /note="Ankyrin repeat protein nuc-2"
FT /id="PRO_0000067064"
FT DOMAIN 1..166
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT REPEAT 268..298
FT /note="ANK 1"
FT REPEAT 336..366
FT /note="ANK 2"
FT REPEAT 370..399
FT /note="ANK 3"
FT REPEAT 403..432
FT /note="ANK 4"
FT REPEAT 435..465
FT /note="ANK 5"
FT REPEAT 470..499
FT /note="ANK 6"
FT REPEAT 503..532
FT /note="ANK 7"
FT DOMAIN 717..1048
FT /note="GP-PDE"
FT REGION 923..963
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 941..963
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 857
FT /note="A -> V (in Ref. 1; AAB03277)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1066 AA; 117115 MW; 1E189EA92FB62B75 CRC64;
MKFGKQIQKR QLEVPEYAAS FVNYKALKKL IKKLSATPIL PPQTDLRRAP GEPLDTQSAL
QANKATFFFQ IDRELDKVNA CYVQKEAELK IRLKTLLDKK KALRSRSGGT SRRSTKFTTL
QEGFQQFVND LNKLQQFVEI NGTAFSKILK KWDKTAKSKT KELYLSRVVE KRPAFNPTVI
SELSDQATTS LQELGAWADG DNVSFETRPD HDHVVSTQHL LGTDEGDADT LLLDTVLSGN
IDSLKDMLDR MKATAAPDGS ADVSLAERVT RTFLASINEG SLEALKVLLD TGLVDIQSWD
DINERNCLHQ AAIYGNSFVL EYGLSKGVDA ERTDAYGRVP LHYASMHGRL DMIDALLNAS
PKTINLIDHD NFTPLVHSIV RNHLECVGRL LERSARIDPV SDTDHVPLNL ACQHGSVAIV
ELLLKHGAKI LADAEGLYPQ HLVARSGQTP EILVLLKQYG ADLDQIDKLY GWTPLVHAAS
EGNVPCLQAL LETGADPNIL DEKDLPAMYY AAWEGHLECM KLLTPAKKEK AASELPPIHI
GGALPPMASS TAPMPMSLDA IDPIPALELP PPIIPLRRYG HNFLDTKTVV QISFEEDSEQ
PLLFFQDGKY PAARLTISSK SSDLIPKNII LPFQEDTRVA SFQIDNLESF TLDFEVFPTY
GAKVIAKTVA LPNIFRALLS SSGKCCLPLF DPRLRAIGQI SFHVQIIKPF SGTPLEITDF
ETYWKATSQF DTNTSTFVTG SSLSGDFVQI YVQHTKDGVP VLWPRWTINC GGIDVPVSTL
TLAQFQTVTA AARNRINLSE LSTYTLDQIA DVHRILANIG ITLHEALFLL PKGMHVNIQV
LYPTADEKAE AKSSTAADDV NEFADAILSV VFDHARAQRA ERPDSVRSVV FSSYNPTLCT
ALNWKQPNFP VFLCNDMGRE DRKQQQQGSC SKGDGDEDMG GTTAASRREA ADERTLQSDG
RRTSSIKDVV RTATSNNLMG LICCSRLLDM VPALVDAIKS HGLALVVDKS GEPEAANSKD
SGDKQLGISG ALGGLAGSDP FPKLPKGVDG LLKSNGVLRF NEYIDV
//
