ID O52273_LACLL Unreviewed; 531 AA.
AC O52273;
DT 01-JUN-1998, integrated into UniProtKB/TrEMBL.
DT 01-JUN-1998, sequence version 1.
DT 27-MAR-2024, entry version 89.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN Name=HsdM {ECO:0000313|EMBL:AAB91416.1};
OS Lactococcus lactis subsp. lactis bv. diacetylactis.
OG Plasmid pND861 {ECO:0000313|EMBL:AAB91416.1}.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=44688 {ECO:0000313|EMBL:AAB91416.1};
RN [1] {ECO:0000313|EMBL:AAB91416.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=UK19161 {ECO:0000313|EMBL:AAB91416.1};
RC PLASMID=pND861 {ECO:0000313|EMBL:AAB91416.1};
RX PubMed=11092734;
RA Deng Y.M., Liu C.Q., Dunn N.W.;
RT "LldI, a plasmid-encoded type I restriction and modification system in
RT Lactococcus lactis.";
RL DNA Seq. 11:239-245(2000).
RN [2] {ECO:0000313|EMBL:AAB91416.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=UK19161 {ECO:0000313|EMBL:AAB91416.1};
RC PLASMID=pND861 {ECO:0000313|EMBL:AAB91416.1};
RA Deng Y.-M., Dunn N.W.;
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; AF034786; AAB91416.1; -; Genomic_DNA.
DR PIR; T09460; T09460.
DR AlphaFoldDB; O52273; -.
DR REBASE; 3606; M.LldI.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Plasmid {ECO:0000313|EMBL:AAB91416.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 5..157
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 169..476
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 531 AA; 60725 MW; 773B79ACFAE84429 CRC64;
MATGLNQQLW ASADILRGKM DASEYKNYLL GLIFYKYLSD AQLREVYEQE NGKTDTFPER
STQYAGFMEW YEEDKDDLIE NIQPKQGYFI QPDQLFYHYR IKADNYEFNL TDLQAGFNEL
ERQGEEFSGL FADIDLNSTK LGSNAQQRNV TITEVLRALD EIDLFEHNGD VIGDAYEYLI
GMFAEGAGKK AGEFYTPQAV SRIMSEITSI GQESRAPFHI YDPAMGSGSL MLNIRRYLLN
PNQVHYHGQE LNTTTFNLAR MNLILHGVDK ERMNLNNGDT LDADWPSEEP YQFDSVVMNP
PYSAKWSAAD KFLSDPRFER FGKLAPKSKA DFAFLLHGFY HLKESGTMGI VLPHGVLFRG
AAEGTIRQAL LEMGAIDAVI GLPANIFFGT SIPTTVIILK KNRSRRDVLF IDASQDFEKQ
KNQNVLLDEH IDKIVSTYKK REDIERYAHV ASFDEIQEND FNLNIPRYVD TFEEEEPVDL
VAVNTNLLKI NEELVQQDQT LLSLINDFSE SEENQAMIES MRLLLRGGHD E
//
