ID GCIP_LITPI Reviewed; 206 AA.
AC O73763;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 22-FEB-2023, entry version 93.
DE RecName: Full=Guanylyl cyclase inhibitory protein;
GN Name=GCIP;
OS Lithobates pipiens (Northern leopard frog) (Rana pipiens).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX NCBI_TaxID=8404;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Retina;
RX PubMed=9546678; DOI=10.1046/j.1432-1327.1998.2520591.x;
RA Li N., Fariss R.N., Zhang K., Otto-Bruc A.E., Haeseleer F., Bronson J.D.,
RA Qin N., Yamazaki A., Subbaraya I., Milam A.H., Palczewski K., Baehr W.;
RT "Guanylate-cyclase-inhibitory protein is a frog retinal Ca2+-binding
RT protein related to mammalian guanylate-cyclase-activating proteins.";
RL Eur. J. Biochem. 252:591-599(1998).
CC -!- FUNCTION: Does not stimulate guanylyl cyclase (GC) when free calcium
CC ion concentration is low, but inhibits GC when free calcium ions
CC concentration is elevated.
CC -!- TISSUE SPECIFICITY: Retina; inner segments, somata and synaptic
CC terminals of cone receptors.
CC -!- MISCELLANEOUS: Binds two calcium ions. {ECO:0000250}.
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DR EMBL; AF047884; AAC15878.1; -; mRNA.
DR AlphaFoldDB; O73763; -.
DR SMR; O73763; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR CDD; cd00051; EFh; 2.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR028846; Recoverin.
DR PANTHER; PTHR23055; CALCIUM BINDING PROTEINS; 1.
DR PANTHER; PTHR23055:SF85; SI:CH211-245J22.3; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR PRINTS; PR00450; RECOVERIN.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 2: Evidence at transcript level;
KW Calcium; Lipoprotein; Metal-binding; Myristate; Repeat;
KW Sensory transduction; Vision.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..206
FT /note="Guanylyl cyclase inhibitory protein"
FT /id="PRO_0000073813"
FT DOMAIN 31..49
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 51..86
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 87..122
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 135..170
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 64
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 66
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 68
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 75
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 100
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 102
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 104
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 206 AA; 23653 MW; FDCD8F6BE17DA7D9 CRC64;
MGQVASMPHR CGTYVLELHE WYRKFVEECP SGLITLHEFR QFFSDVTVGE NSSEYAEQIF
RALDNNGDGI VDFREYVTAI SMLAHGTPED KLKWSFKLYD KDGDGAITRS EMLEIMRAVY
KMSVVASLTK VNPMTAEECT NRIFVRLDKD QNAIISLQEF VDGSLGDEWV RQMLECDLST
VEIQKMTKHS HLPARSSRER LFHANT
//
