ID ORT1_YEAST Reviewed; 292 AA.
AC Q12375; D6W2I8; Q92273;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 27-MAR-2024, entry version 166.
DE RecName: Full=Mitochondrial ornithine transporter 1;
GN Name=ORT1; Synonyms=ARG11; OrderedLocusNames=YOR130C;
GN ORFNames=O3299, YOR3299C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX PubMed=8798783; DOI=10.1074/jbc.271.40.25011;
RA Crabeel M., Soetens O., de Rijcke M., Pratiwi R., Pankiewicz R.;
RT "The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial
RT integral membrane protein required for arginine biosynthesis.";
RL J. Biol. Chem. 271:25011-25018(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8904341;
RX DOI=10.1002/(sici)1097-0061(19960315)12:3<281::aid-yea904>3.0.co;2-o;
RA Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C.,
RA Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W.;
RT "Sequencing and analysis of 51 kb on the right arm of chromosome XV from
RT Saccharomyces cerevisiae reveals 30 open reading frames.";
RL Yeast 12:281-288(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9200815;
RX DOI=10.1002/(sici)1097-0061(19970615)13:7<655::aid-yea120>3.0.co;2-i;
RA Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C.,
RA Schwager C., Paces V., Sander C., Ansorge W.;
RT "DNA sequencing and analysis of 130 kb from yeast chromosome XV.";
RL Yeast 13:655-672(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [5]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 105.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP FUNCTION.
RX PubMed=9237680; DOI=10.1016/s0014-5793(97)00630-3;
RA Palmieri L., De Marco V., Iacobazzi V., Palmieri F., Runswick M.J.,
RA Walker J.E.;
RT "Identification of the yeast ARG-11 gene as a mitochondrial ornithine
RT carrier involved in arginine biosynthesis.";
RL FEBS Lett. 410:447-451(1997).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for arginine biosynthesis. Transports ornithine
CC synthesized from glutamate in the mitochondrial matrix to the cytosol,
CC where it is converted to arginine. {ECO:0000269|PubMed:9237680}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 432 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; X87414; CAA60862.1; -; Genomic_DNA.
DR EMBL; X90518; CAA62118.1; -; Genomic_DNA.
DR EMBL; X94335; CAA64049.1; -; Genomic_DNA.
DR EMBL; Z75038; CAA99329.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10904.2; -; Genomic_DNA.
DR PIR; S60997; S60997.
DR RefSeq; NP_014773.4; NM_001183549.4.
DR AlphaFoldDB; Q12375; -.
DR SMR; Q12375; -.
DR BioGRID; 34525; 32.
DR DIP; DIP-8953N; -.
DR STRING; 4932.YOR130C; -.
DR TCDB; 2.A.29.9.2; the mitochondrial carrier (mc) family.
DR MaxQB; Q12375; -.
DR PaxDb; 4932-YOR130C; -.
DR PeptideAtlas; Q12375; -.
DR EnsemblFungi; YOR130C_mRNA; YOR130C; YOR130C.
DR GeneID; 854297; -.
DR KEGG; sce:YOR130C; -.
DR AGR; SGD:S000005656; -.
DR SGD; S000005656; ORT1.
DR VEuPathDB; FungiDB:YOR130C; -.
DR eggNOG; KOG0758; Eukaryota.
DR GeneTree; ENSGT00940000168956; -.
DR HOGENOM; CLU_015166_16_3_1; -.
DR InParanoid; Q12375; -.
DR OMA; PIDCFRQ; -.
DR OrthoDB; 4064096at2759; -.
DR BioCyc; YEAST:G3O-33654-MONOMER; -.
DR Reactome; R-SCE-70635; Urea cycle.
DR BioGRID-ORCS; 854297; 9 hits in 10 CRISPR screens.
DR PRO; PR:Q12375; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12375; Protein.
DR GO; GO:0005740; C:mitochondrial envelope; IDA:SGD.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0000064; F:L-ornithine transmembrane transporter activity; IDA:SGD.
DR GO; GO:0006526; P:arginine biosynthetic process; IMP:SGD.
DR GO; GO:1990575; P:mitochondrial L-ornithine transmembrane transport; IDA:SGD.
DR Gene3D; 1.50.40.10; Mitochondrial carrier domain; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR PANTHER; PTHR45624; MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED; 1.
DR PANTHER; PTHR45624:SF31; MITOCHONDRIAL ORNITHINE TRANSPORTER 1; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; Mitochondrial carrier; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Amino-acid biosynthesis; Arginine biosynthesis; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..292
FT /note="Mitochondrial ornithine transporter 1"
FT /id="PRO_0000090684"
FT TRANSMEM 14..34
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..187
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT REPEAT 11..97
FT /note="Solcar 1"
FT REPEAT 105..196
FT /note="Solcar 2"
FT REPEAT 211..292
FT /note="Solcar 3"
FT CONFLICT 105
FT /note="S -> F (in Ref. 2; CAA62118, 3; CAA64049 and 4;
FT CAA99329)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 292 AA; 31520 MW; 3B80DDCC5875FD66 CRC64;
MEDSKKKGLI EGAILDIING SIAGACGKVI EFPFDTVKVR LQTQASNVFP TTWSCIKFTY
QNEGIARGFF QGIASPLVGA CLENATLFVS YNQCSKFLEK HTNVSPLGQI LISGGVAGSC
ASLVLTPVEL VKCKLQVANL QVASAKTKHT KVLPTIKAII TERGLAGLWQ GQSGTFIRES
FGGVAWFATY EIVKKSLKDR HSLDDPKRDE SKIWELLISG GSAGLAFNAS IFPADTVKSV
MQTEHISLTN AVKKIFGKFG LKGFYRGLGI TLFRAVPANA AVFYIFETLS AL
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