ID SPE2C_STRPU Reviewed; 151 AA.
AC P04111;
DT 01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 2.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=Calcium-binding protein SPEC 2C;
GN Name=SPEC2C;
OS Strongylocentrotus purpuratus (Purple sea urchin).
OC Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC Euechinoidea; Echinacea; Camarodonta; Echinidea; Strongylocentrotidae;
OC Strongylocentrotus.
OX NCBI_TaxID=7668;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6697391; DOI=10.1016/0092-8674(84)90346-5;
RA Carpenter C.D., Bruskin A.M., Hardin P.E., Keast M.J., Anstrom J.A.,
RA Tyner A.L., Brandhorst B.P., Klein W.H.;
RT "Novel proteins belonging to the troponin C superfamily are encoded by a
RT set of mRNAs in sea urchin embryos.";
RL Cell 36:663-671(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3172223; DOI=10.1016/0022-2836(88)90275-6;
RA Hardin P.E., Angerer L.M., Hardin S.H., Angerer R.C., Klein W.H.;
RT "Spec2 genes of Strongylocentrotus purpuratus. Structure and differential
RT expression in embryonic aboral ectoderm cells.";
RL J. Mol. Biol. 202:417-431(1988).
CC -!- FUNCTION: Calcium-binding protein involved in larval development and
CC metamorphosis. Likely to function as calcium buffers mediating the
CC transport of calcium from the sea water to the blastocoel where calcium
CC is required for skeleton formation.
CC -!- TISSUE SPECIFICITY: Found in cell lineages giving rise to the aboral
CC ectoderm, a squamous epithelium covering the surface of the late stage
CC embryo and larva.
CC -!- DEVELOPMENTAL STAGE: Accumulate in embryos and larvae, but not in
CC adults.
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DR EMBL; X12772; CAA31260.2; -; Genomic_DNA.
DR EMBL; X14534; CAA31260.2; JOINED; Genomic_DNA.
DR EMBL; X14535; CAA31260.2; JOINED; Genomic_DNA.
DR EMBL; X14536; CAA31260.2; JOINED; Genomic_DNA.
DR EMBL; X14537; CAA31260.2; JOINED; Genomic_DNA.
DR EMBL; X14538; CAA31260.2; JOINED; Genomic_DNA.
DR PIR; S01771; MCUR2C.
DR RefSeq; NP_999769.1; NM_214604.1.
DR AlphaFoldDB; P04111; -.
DR SMR; P04111; -.
DR STRING; 7668.P04111; -.
DR EnsemblMetazoa; NM_214604; NP_999769; GeneID_373447.
DR GeneID; 373447; -.
DR KEGG; spu:373447; -.
DR CTD; 373447; -.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_2609113_0_0_1; -.
DR InParanoid; P04111; -.
DR OMA; CRCEFTE; -.
DR OrthoDB; 4490703at2759; -.
DR PhylomeDB; P04111; -.
DR Proteomes; UP000007110; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 2.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR PANTHER; PTHR46212; PEFLIN; 1.
DR PANTHER; PTHR46212:SF3; PEFLIN; 1.
DR Pfam; PF13499; EF-hand_7; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..151
FT /note="Calcium-binding protein SPEC 2C"
FT /id="PRO_0000073640"
FT DOMAIN 10..45
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 46..78
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 81..116
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 118..151
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 23
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 29
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 34
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 59
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 61
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 63
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 70
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 94
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 96
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 98
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 100
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 105
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 131
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000305"
FT BINDING 135
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000305"
FT BINDING 137
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000305"
FT BINDING 142
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000305"
FT CONFLICT 45
FT /note="E -> D (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="E -> L (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 137
FT /note="E -> K (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 140..141
FT /note="RA -> VQ (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="Q -> E (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 151 AA; 16991 MW; 0ACAAC12A3E3E1B9 CRC64;
MAVQLFFTEE QRKVFKSSFK SIDADGDGKI TPEELKAAFK SIEIELTQEK IDEMMSMVDK
DGSRPVDFSE ILMKKAEQMR GKGAQYFKAF DALDTDKSGS LSPEELRTAL SACTDPPMTK
EEIDAIIKKA DGNNDGEIRR AEFVRMIQSS Y
//
