ID FATB_VIBA7 Reviewed; 322 AA.
AC P11460;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 27-MAR-2024, entry version 111.
DE RecName: Full=Ferric-anguibactin-binding protein FatB {ECO:0000305};
DE Flags: Precursor;
GN Name=fatB {ECO:0000303|PubMed:1748657};
OS Vibrio anguillarum (strain ATCC 68554 / 775) (Listonella anguillarum).
OG Plasmid pJM1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=882102;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 68554 / 775;
RX PubMed=2830268; DOI=10.1016/s0021-9258(18)69147-1;
RA Actis L.A., Tolmasky M.E., Farrell D.H., Crosa J.H.;
RT "Genetic and molecular characterization of essential components of the
RT Vibrio anguillarum plasmid-mediated iron-transport system.";
RL J. Biol. Chem. 263:2853-2860(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 68554 / 775;
RX PubMed=13129954; DOI=10.1128/jb.185.19.5822-5830.2003;
RA Di Lorenzo M., Stork M., Tolmasky M.E., Actis L.A., Farrell D., Welch T.J.,
RA Crosa L.M., Wertheimer A.M., Chen Q., Salinas P., Waldbeser L., Crosa J.H.;
RT "Complete sequence of virulence plasmid pJM1 from the marine fish pathogen
RT Vibrio anguillarum strain 775.";
RL J. Bacteriol. 185:5822-5830(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 68554 / 775;
RX PubMed=21576332; DOI=10.1128/iai.05138-11;
RA Naka H., Dias G.M., Thompson C.C., Dubay C., Thompson F.L., Crosa J.H.;
RT "Complete genome sequence of the marine fish pathogen Vibrio anguillarum
RT harboring the pJM1 virulence plasmid and genomic comparison with other
RT virulent strains of V. anguillarum and V. ordalii.";
RL Infect. Immun. 79:2889-2900(2011).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154, DISRUPTION PHENOTYPE, FUNCTION,
RP AND SUBUNIT.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=1748657; DOI=10.1016/s0021-9258(18)54358-1;
RA Koester W.L., Actis L.A., Waldbeser L.S., Tolmasky M.E., Crosa J.H.;
RT "Molecular characterization of the iron transport system mediated by the
RT pJM1 plasmid in Vibrio anguillarum 775.";
RL J. Biol. Chem. 266:23829-23833(1991).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF CYS-23.
RC STRAIN=ATCC 68554 / 775;
RX PubMed=21304833; DOI=10.1111/j.1758-2229.2009.00110.x;
RA Naka H., Lopez C.S., Crosa J.H.;
RT "Role of the pJM1 plasmid-encoded transport proteins FatB, C and D in
RT ferric anguibactin uptake in the fish pathogen Vibrio anguillarum.";
RL Environ. Microbiol. Rep. 2:104-111(2010).
CC -!- FUNCTION: Involved in the uptake of iron in complex with the
CC siderophore anguibactin. Binds ferric-anguibactin in the periplasm and
CC mediates its transport into the cytoplasm. {ECO:0000269|PubMed:1748657,
CC ECO:0000269|PubMed:21304833}.
CC -!- SUBUNIT: Part of an iron transport system composed of the outer
CC membrane receptor FatA, the periplasmic binding protein FatB and the
CC inner membrane proteins FatC and FatD. {ECO:0000305|PubMed:1748657}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:21304833}; Lipid-anchor
CC {ECO:0000269|PubMed:21304833}; Periplasmic side
CC {ECO:0000269|PubMed:21304833}. Note=The lipoprotein nature of FatB is
CC not essential for activity. {ECO:0000269|PubMed:21304833}.
CC -!- DISRUPTION PHENOTYPE: Mutants are unable to grow under iron-limiting
CC conditions. {ECO:0000269|PubMed:1748657}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 8 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY312585; AAR12526.1; -; Genomic_DNA.
DR PIR; A29928; A29928.
DR RefSeq; NP_943551.1; NC_005250.1.
DR RefSeq; WP_011154637.1; NC_005250.1.
DR AlphaFoldDB; P11460; -.
DR SMR; P11460; -.
DR eggNOG; COG4607; Bacteria.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006826; P:iron ion transport; IEA:UniProtKB-KW.
DR CDD; cd01140; FatB; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 2.
DR InterPro; IPR002491; ABC_transptr_periplasmic_BD.
DR InterPro; IPR033870; FatB.
DR PANTHER; PTHR30532:SF1; FE(3+) DICITRATE-BINDING PERIPLASMIC PROTEIN; 1.
DR PANTHER; PTHR30532; IRON III DICITRATE-BINDING PERIPLASMIC PROTEIN; 1.
DR Pfam; PF01497; Peripla_BP_2; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS50983; FE_B12_PBP; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Ion transport; Iron; Iron transport;
KW Lipoprotein; Membrane; Palmitate; Plasmid; Signal; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 23..322
FT /note="Ferric-anguibactin-binding protein FatB"
FT /id="PRO_0000031819"
FT DOMAIN 57..322
FT /note="Fe/B12 periplasmic-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00344"
FT LIPID 23
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 23
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT MUTAGEN 23
FT /note="C->A: Localizes mainly in the periplasmic fraction
FT and not in the membrane fraction. Does not affect ferric-
FT anguibactin uptake."
FT /evidence="ECO:0000269|PubMed:21304833"
SQ SEQUENCE 322 AA; 35635 MW; FB8674EED5CF73F7 CRC64;
MFKSTLNIAV AIVCSSLVTL TGCEPKVAQS QVIQPLETPI VIEHNLGQTV ISNRPQRVAA
LDMNEVDFLD QLNVPIAGMV KDFVPHFLEK YKNTPDISDL GAIVQPNMEK IYALKPDLVL
MTPLHANQYE ELSKLAPTVH FDIDFRNSHG HHVDIIKQHV IDLGEIFNKQ TLAQKKVAEI
DAKVDEVQAL TAERSEKALV VMHNNGSFSS FGIESRYGFV FDVLGVKPAS TEIAASLHGQ
PISSEFINQA NPDILYIIDR TAVMEGKPVI DAEHLANPLL RQTKAWKNGK VIFVDADAWY
ITSASITSLK IVIDDIIKGY QS
//
