ID YCF78_CHLRE Reviewed; 1995 AA.
AC P36495; B7U1J9; Q37303;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 24-JAN-2024, entry version 88.
DE RecName: Full=Uncharacterized membrane protein ycf78;
DE AltName: Full=ORF-S;
DE AltName: Full=ORF1995;
DE AltName: Full=ORFA;
GN Name=ycf78;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9065699; DOI=10.1007/s004380050368;
RA Boudreau E., Turmel M., Goldschmidt-Clermont M., Rochaix J.-D., Sivan S.,
RA Michaels A., Leu S.;
RT "A large open reading frame (orf1995) in the chloroplast DNA of
RT Chlamydomonas reinhardtii encodes an essential protein.";
RL Mol. Gen. Genet. 253:649-653(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1573-1995.
RC STRAIN=cw15;
RA Sivan S., Michaels A.;
RL Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1897-1995.
RX PubMed=2876928; DOI=10.1016/0378-1119(86)90038-7;
RA Woessner J.P., Gillham N.W., Boynton J.E.;
RT "The sequence of the chloroplast atpB gene and its flanking regions in
RT Chlamydomonas reinhardtii.";
RL Gene 44:17-28(1986).
RN [5]
RP PARTIAL NUCLEOTIDE SEQUENCE.
RX PubMed=8155879; DOI=10.1007/bf00023556;
RA Boudreau E., Otis C., Turmel M.;
RT "Conserved gene clusters in the highly rearranged chloroplast genomes of
RT Chlamydomonas moewusii and Chlamydomonas reinhardtii.";
RL Plant Mol. Biol. 24:585-602(1994).
RN [6]
RP IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
CC -!- FUNCTION: Essential for cell growth. May be involved in binding
CC chloroplast DNA to either the chloroplast envelope or the thylakoid
CC membrane.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ycf78 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA84144.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X92726; CAA63385.1; -; Genomic_DNA.
DR EMBL; M13704; AAA84144.1; ALT_INIT; Genomic_DNA.
DR EMBL; FJ423446; ACJ50146.1; -; Genomic_DNA.
DR EMBL; X76934; CAA54257.1; -; Genomic_DNA.
DR EMBL; BK000554; DAA00959.1; -; Genomic_DNA.
DR PIR; S41289; S41289.
DR PIR; T08166; T08166.
DR RefSeq; NP_958415.1; NC_005353.1.
DR PDB; 7VCF; EM; 2.50 A; A=1-1995.
DR PDB; 7XZI; EM; 2.77 A; A=1-1995.
DR PDB; 7XZJ; EM; 2.97 A; A=1-1995.
DR PDBsum; 7VCF; -.
DR PDBsum; 7XZI; -.
DR PDBsum; 7XZJ; -.
DR AlphaFoldDB; P36495; -.
DR EMDB; EMD-31890; -.
DR EMDB; EMD-33528; -.
DR EMDB; EMD-33529; -.
DR SMR; P36495; -.
DR STRING; 3055.P36495; -.
DR PaxDb; 3055-DAA00959; -.
DR GeneID; 2717035; -.
DR KEGG; cre:ChreCp059; -.
DR HOGENOM; CLU_234019_0_0_1; -.
DR InParanoid; P36495; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; Plastid; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..1995
FT /note="Uncharacterized membrane protein ycf78"
FT /id="PRO_0000217502"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1418..1441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1848..1883
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1422..1441
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1848..1868
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 580
FT /note="L -> V (in strain: CC-503)"
FT VARIANT 1588
FT /note="K -> R (in strain: CC-503 and cw15)"
FT VARIANT 1610
FT /note="P -> A (in strain: CC-503)"
FT VARIANT 1618
FT /note="P -> A (in strain: CC-503)"
FT HELIX 9..22
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 23..26
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 35..54
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 55..59
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 72..74
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 106..122
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 128..139
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 142..165
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 168..176
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 178..196
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 197..199
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 206..220
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 226..228
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 229..232
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 236..242
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 251..281
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 283..292
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 301..303
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 304..321
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 322..326
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 329..337
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 344..346
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 349..351
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 356..358
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 360..362
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 374..376
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 386..388
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 390..393
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 408..410
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 412..416
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 418..424
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 425..429
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 436..438
FT /evidence="ECO:0007829|PDB:7XZI"
FT HELIX 440..449
FT /evidence="ECO:0007829|PDB:7XZI"
FT TURN 468..470
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 471..479
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 483..486
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 540..543
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 546..548
FT /evidence="ECO:0007829|PDB:7XZI"
FT HELIX 560..583
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 586..588
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 599..601
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 602..610
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 614..616
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 617..633
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 642..645
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 646..648
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 653..667
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 681..697
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 698..700
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 714..723
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 732..738
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 744..746
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 755..758
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 808..810
FT /evidence="ECO:0007829|PDB:7XZI"
FT HELIX 815..833
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 836..838
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 840..849
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 850..852
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 855..857
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 861..879
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 883..886
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 892..896
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 903..905
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 913..915
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 916..919
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 920..922
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 929..931
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 951..953
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 955..958
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 968..980
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1047..1062
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1066..1103
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1131..1142
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1144..1147
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1156..1180
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1232..1244
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 1245..1248
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1252..1261
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1262..1264
FT /evidence="ECO:0007829|PDB:7XZJ"
FT HELIX 1272..1276
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 1281..1283
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 1348..1352
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1357..1359
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 1378..1381
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 1383..1389
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1391..1393
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1398..1411
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 1412..1414
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1415..1418
FT /evidence="ECO:0007829|PDB:7XZI"
FT TURN 1427..1430
FT /evidence="ECO:0007829|PDB:7XZI"
FT HELIX 1431..1435
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1436..1438
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1439..1447
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1454..1489
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1500..1503
FT /evidence="ECO:0007829|PDB:7XZI"
FT HELIX 1547..1558
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 1564..1566
FT /evidence="ECO:0007829|PDB:7XZI"
FT STRAND 1580..1583
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1585..1587
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1589..1593
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 1599..1604
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1608..1611
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1616..1619
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1625..1630
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 1631..1633
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1636..1639
FT /evidence="ECO:0007829|PDB:7XZI"
FT HELIX 1640..1643
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1647..1653
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 1664..1666
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1691..1693
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1699..1718
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1721..1724
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 1725..1730
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1742..1744
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1746..1752
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1759..1761
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1774..1786
FT /evidence="ECO:0007829|PDB:7VCF"
FT TURN 1787..1789
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1804..1806
FT /evidence="ECO:0007829|PDB:7VCF"
FT STRAND 1922..1924
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1956..1970
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1974..1976
FT /evidence="ECO:0007829|PDB:7VCF"
FT HELIX 1977..1988
FT /evidence="ECO:0007829|PDB:7VCF"
SQ SEQUENCE 1995 AA; 232195 MW; E5A3F167EAF5FC8A CRC64;
MITFTFMSLV TSVKDYVEIT HKLIEIEPLK NYTEFGAVFT YFIFSIGEFF KNFFSFSFLN
NIWSIPIIIP DIASAMISEV SVLDGYFHNA FTFLETSVNT TTNPSLVIFE KFVIGIINSL
FLILPTSTSH LITLRRFVMQ GLEAGYMAGL GTLAGNFLWL ASIILGWRFF VIPWLSLDIF
RYLLGFVLLV KYIWDSSKER RMALEDLSKW KIFLLNFLLA LTEQSCIYPF ISNLSFGPDA
SILEGFPVDN YPQFLLIHGA YLLGILFGSF SLLQFTCWFW ENPAFSIYLW ITTKSSLKIS
TSSYYKILNF TFLYATMLCA IASIPYYGLD YTITNPIGLV PQDRILNQKK SQSDPDKLIT
ETAFLNLNPT DKNSRIRDGV HARRERWKQR LIKYQAFDAS TYDQGVYDFL TIEDLNYGFD
RFWLRRKMRN HQIRFRLFPG PWMRSLKKQL NNPANPSLET STKAASGPRV EFFRILFEQF
YHPNFHDRAA MQTNPAEARN KFISTSPLAS TESKKALNST FSLGNINNSS TGIEGLVLTN
TQATLLPTDL QTKRTIKPGL IYTNSALRKF VRNVNTRLNL KLLNSKETNL TTKYKSQFIY
SKRWKSIFSK IQPLQNGTTR KSYQLFRNVA KQILVTPDAK SLKLITINQK LSLKERKLLE
LRTQYNNNST LTTTAPLTLV RPLNVYLQKE EAFKRKLRYY GTMPMRKLTV GNQAPYFKAL
MKRGFYYYKP TLRWRKTLYV ASLRRGFRKK SRKQRILVMP SNQQNFNNTL DNTKTNINQN
NLANPLGGNE VPMYGADGEN SLITKPTHSY TVLGKRASRY RHQIYKDVLQ HWYYTPFNRL
LMKFDVDAFI NRQPKSHFLT KNEERALHIR RFLLSEHYDT LRWYTYMQHY KTMKTNIGGT
KSFANRAYNQ QFQGTFKKIR HLFAITPKQG DFYTLKFDQP LYNDNKLKDN LYFHEELLTD
YYNGTNLQTN QTSNISVNST TTFIDNSLRT TQLPVPSSSF DIVNQSSTLI GLTTMQNALR
KNVVESTLTS LNSDGEAATS QPKLNFVYSE LFVKLIKECK KRIHDQTFLK NYITHRIEKR
EQLNQEQTKE LNKRLEKLKV WLNSDKGSIS KLQNTPVQDP NISSPDKVLT TAMQKAVNES
ISLSGIMPSD KIKTTYGNLT NAYTIKTENA ILTKLNVINQ LTNNETTTQK NTLIKSIGVN
KIQTVLQTII TNFKSSLYNQ TQLLRVKTDK DLQWWRTKQR VITKRKSARK RDRFKKQIAV
VNKKLAALSK KVETEKSNLY QTLYGNYEIS DYLLRNVPTG SSAVIDSTVL RKKQDNQAYL
PKETNNVQFN SFVDSNNNVW QTFFAKKLRK KISSKGRRYR SLSLARYLTA TRKPRLVGLD
NLTKIDNITT LQGAFITKEE KQDSLNLTIQ RKQELTNSLK KSQIKKRSRH SWKKRSRHQF
SRNHYKYRKR HTHGNGKLRV MNKKLKKFKA TNELRQWWWN SFLPRYLSNL QVNNSTLTNK
NVSFKPLSNT NSVPSTNMAS PTTSRNLLDN LNSSNQISTS ASMNQNIVTE SVKVETNQVY
LPEGEKSFDI TSMTTTLPFY AGWDESLKKF VVTNRLLSRR DAGLSVNNNP QEINFTNPPI
QGLNEGSFLY WQTEMPFNSY NIDQFITTNQ SFYAPLGWRR FEFRHSILKT WVNNTKAGNN
NIKKKTLIIS LKNLQPLKSS QQKQNQIKTK KLVARRIKKR YKLLKQMPNQ LMYSPTGPLL
TEVLPSHYIS VFDQQYRLPR NRYLKRNPLK TLKKTTLLAL MDSSKQTNGV NKEFTLRKRV
KPRRKYHRKR FIKKDGLIFP RRTKFNTNTT LTGNALITNN VNSIEEDDLR WRPSSRTKQK
RKDNTRSSAA SKTKSNKRVK TNPLRLRQLR RREFQQVLKP LQRYIPQNGG FTWPGDYLRL
EIVEMPKLKS INIKKTSLKQ KINVQPVGIM PRKYLIEKHN IKVLKKKLSQ AYSTQQLTKV
VQEYKNLIQN SPPAI
//
