ID NRTB_SYNE7 Reviewed; 279 AA.
AC P38044; Q31NV1;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 27-MAR-2024, entry version 121.
DE RecName: Full=Nitrate import permease protein NrtB {ECO:0000305};
GN Name=nrtB {ECO:0000303|PubMed:8437564}; OrderedLocusNames=Synpcc7942_1238;
OS Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)
OS (Anacystis nidulans R2).
OC Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Synechococcaceae;
OC Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8437564; DOI=10.1007/bf00277112;
RA Omata T., Andriesse X., Hirano A.;
RT "Identification and characterization of a gene cluster involved in nitrate
RT transport in the cyanobacterium Synechococcus sp. PCC7942.";
RL Mol. Gen. Genet. 236:193-202(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33912 / PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Golden S., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 33912 / PCC 7942 / FACHB-805;
RX PubMed=7767600; DOI=10.1093/oxfordjournals.pcp.a078751;
RA Omata T.;
RT "Structure, function and regulation of the nitrate transport system of the
RT cyanobacterium Synechococcus sp. PCC7942.";
RL Plant Cell Physiol. 36:207-213(1995).
CC -!- FUNCTION: Part of the ABC transporter complex NrtABCD involved in
CC nitrate uptake (PubMed:7767600, PubMed:8437564). The complex is
CC probably also involved in nitrite transport (PubMed:7767600). Probably
CC responsible for the translocation of the substrate across the membrane
CC (PubMed:7767600). {ECO:0000269|PubMed:8437564,
CC ECO:0000305|PubMed:7767600}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NrtC and
CC NrtD), two transmembrane proteins (NrtB) and a solute-binding protein
CC (NrtA). {ECO:0000305|PubMed:7767600}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:7767600};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Mutant is unable to grow on nitrate, but grows
CC normally with nitrite or ammonium as the nitrogen source.
CC {ECO:0000269|PubMed:8437564}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. CysTW subfamily. {ECO:0000305}.
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DR EMBL; X61625; CAA43810.1; -; Genomic_DNA.
DR EMBL; CP000100; ABB57268.1; -; Genomic_DNA.
DR PIR; S30892; S30892.
DR RefSeq; WP_011242626.1; NZ_JACJTX010000003.1.
DR AlphaFoldDB; P38044; -.
DR SMR; P38044; -.
DR STRING; 1140.Synpcc7942_1238; -.
DR TCDB; 3.A.1.16.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; 1140-Synpcc7942_1238; -.
DR GeneID; 76399980; -.
DR KEGG; syf:Synpcc7942_1238; -.
DR eggNOG; COG0600; Bacteria.
DR HOGENOM; CLU_046113_1_1_3; -.
DR OrthoDB; 9804353at2; -.
DR BioCyc; SYNEL:SYNPCC7942_1238-MONOMER; -.
DR Proteomes; UP000889800; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006811; P:monoatomic ion transport; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3720.10; MetI-like; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR InterPro; IPR005889; NtrB.
DR NCBIfam; TIGR01183; ntrB; 1.
DR PANTHER; PTHR30151; ALKANE SULFONATE ABC TRANSPORTER-RELATED, MEMBRANE SUBUNIT; 1.
DR PANTHER; PTHR30151:SF7; NITRATE IMPORT PERMEASE PROTEIN NRTB; 1.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; MetI-like; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..279
FT /note="Nitrate import permease protein NrtB"
FT /id="PRO_0000060126"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 84..267
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 279 AA; 30207 MW; 5565FE001113A7AE CRC64;
MTVTLRPPSS VRRSAWVKNP KLKPFLPYVV CLPIFLAIWQ VISAILGQDR LPGPINVVAN
TWMPYIVEPF FDNGGTSKGL GLQILISLQR VAIGYLLAAC TGILVGGVLG MSKFLGKGLD
PVIQVLRTVP PLAWFPISLM VFQDANTSAI FVIFITAIWP IIINTAVGIN QIPDDYNNVA
RVLKLSKKDY ILNILIPSTV PYVFAGLRIA VGLAWLAIVA AEMLKADGGI GYFIWDAYNA
GGDGSSSQII LAIFYVGLVG LSLDRLVAWV GRLVSPVSR
//
