ID RECG_TREPA Reviewed; 686 AA.
AC P96130;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 27-MAR-2024, entry version 137.
DE RecName: Full=ATP-dependent DNA helicase RecG;
DE EC=3.6.4.12;
GN Name=recG; OrderedLocusNames=TP_0687;
OS Treponema pallidum (strain Nichols).
OC Bacteria; Spirochaetota; Spirochaetia; Spirochaetales; Treponemataceae;
OC Treponema.
OX NCBI_TaxID=243276;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Nichols;
RA Stamm L.V., Barnes N.Y.;
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nichols;
RX PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M., Utterback T.R.,
RA McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A.,
RA Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O.,
RA Venter J.C.;
RT "Complete genome sequence of Treponema pallidum, the syphilis spirochete.";
RL Science 281:375-388(1998).
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA) (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000305}.
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DR EMBL; U70661; AAB38707.1; -; Genomic_DNA.
DR EMBL; AE000520; AAC65656.1; -; Genomic_DNA.
DR PIR; D71292; D71292.
DR RefSeq; WP_010882132.1; NC_021490.2.
DR AlphaFoldDB; P96130; -.
DR SMR; P96130; -.
DR IntAct; P96130; 5.
DR STRING; 243276.TP_0687; -.
DR EnsemblBacteria; AAC65656; AAC65656; TP_0687.
DR GeneID; 57879212; -.
DR KEGG; tpa:TP_0687; -.
DR eggNOG; COG1200; Bacteria.
DR HOGENOM; CLU_005122_7_1_12; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000000811; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd17992; DEXHc_RecG; 1.
DR CDD; cd18811; SF2_C_RecG; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR045562; RecG_dom3_C.
DR InterPro; IPR033454; RecG_wedge.
DR NCBIfam; TIGR00643; recG; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_dom3_C; 1.
DR Pfam; PF17191; RecG_wedge; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..686
FT /note="ATP-dependent DNA helicase RecG"
FT /id="PRO_0000102161"
FT DOMAIN 285..453
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 472..632
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 399..402
FT /note="DEQH box"
FT BINDING 298..305
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 686 AA; 77197 MW; B05AFCDC6FA046B0 CRC64;
MLIGEIKEPV SVLKGTGKVV LAQLERLNIS TIGDILSYWP RLWEDRTQEQ MFSQWTLAHR
LQVRVSVTAH CWFGFGKSKT LKLVVQDGQG CVAELLCFRR NFLHFMFPVG SEAVVYGSFY
EKDGLLESSS FDIEKIDCIE KKILPVYPLT KGLKQMKLRM LICAAMDQWI GTVDSELPKP
ILEKYHLLTK RDVLLSMHTP RTLDDVAKGK HSLIFEEFFS LQMTIGMRSL QKRGRLPLTQ
GESDQQSAIP SVVSELSLLQ KKLHRCLPFE LTVDQKRVIT EITQDLEREE PMARLIQGDV
GSGKTLVAFF SCLKIIEQGG QVALLAPTEL LARQHADTAA RLLAPIGIRL AFLTGNVKSE
GRAYLLEALV AGEINLVVGT HALFSKSVRY HDLRLVIIDE QHRFGVLQRS ALIQKGREGN
PQGKTPHIIM MSATPIPRTL ALSVFGDLDI SIIKSMPGGR KPVITYIARK TKAEKVYEFV
GNEIEKGRQA YFIYPRIHDI GLTDLKSVQC MYMYLKNYFA RYAVAMIHSK MTEEEQQRIM
KYFSEGTVHI LVATSVVEVG VDVPNANCIV IEHAERFGLS ALHQLRGRVG RGDVQSYCFL
MHGDEMTECA KRRLKIMGST ADGFVIAEED LKLRGPGDVG DTKNFEQSGY SGFRVADPVR
DYPILQVARE AAFELLRKEK GSSEAR
//
