ID PHLN_PSEAE Reviewed; 692 AA.
AC P15713;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 27-MAR-2024, entry version 132.
DE RecName: Full=Non-hemolytic phospholipase C;
DE EC=3.1.4.3;
DE AltName: Full=PLC-N;
DE AltName: Full=Phosphatidylcholine cholinephosphohydrolase;
DE AltName: Full=Phosphatidylcholine-hydrolyzing phospholipase C;
DE Short=PC-PLC;
DE Flags: Precursor;
GN Name=plcN; OrderedLocusNames=PA3319;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2120196; DOI=10.1128/jb.172.10.5915-5923.1990;
RA Ostroff R.M., Vasil A.I., Vasil M.L.;
RT "Molecular comparison of a nonhemolytic and a hemolytic phospholipase C
RT from Pseudomonas aeruginosa.";
RL J. Bacteriol. 172:5915-5923(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Hydrolyzes phosphatidylserine as well as phosphatidylcholine.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC sn-glycerol + H(+) + phosphocholine; Xref=Rhea:RHEA:10604,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:295975; EC=3.1.4.3;
CC -!- PTM: Predicted to be exported by the Tat system. The position of the
CC signal peptide cleavage has not been experimentally proven.
CC -!- SIMILARITY: Belongs to the bacterial phospholipase C family.
CC {ECO:0000305}.
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DR EMBL; M59304; AAA25968.1; -; Genomic_DNA.
DR EMBL; M30223; AAA25969.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06707.1; -; Genomic_DNA.
DR PIR; A36143; A36143.
DR PIR; E83230; E83230.
DR RefSeq; NP_252009.1; NC_002516.2.
DR RefSeq; WP_003113149.1; NZ_QZGE01000017.1.
DR AlphaFoldDB; P15713; -.
DR SMR; P15713; -.
DR STRING; 208964.PA3319; -.
DR PaxDb; 208964-PA3319; -.
DR GeneID; 882484; -.
DR KEGG; pae:PA3319; -.
DR PATRIC; fig|208964.12.peg.3476; -.
DR PseudoCAP; PA3319; -.
DR HOGENOM; CLU_008770_1_0_6; -.
DR InParanoid; P15713; -.
DR OrthoDB; 9770871at2; -.
DR PhylomeDB; P15713; -.
DR BioCyc; PAER208964:G1FZ6-3383-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016298; F:lipase activity; IDA:PseudoCAP.
DR GO; GO:0034480; F:phosphatidylcholine phospholipase C activity; IEA:UniProtKB-EC.
DR GO; GO:0016042; P:lipid catabolic process; IEA:InterPro.
DR GO; GO:0015628; P:protein secretion by the type II secretion system; IDA:PseudoCAP.
DR CDD; cd16014; PLC; 1.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 2.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR017767; Bact_PC-PLC.
DR InterPro; IPR007312; Phosphoesterase.
DR InterPro; IPR008475; PLipase_C_C.
DR InterPro; IPR006311; TAT_signal.
DR NCBIfam; TIGR03396; PC_PLC; 1.
DR PANTHER; PTHR31956:SF8; ACID PHOSPHATASE PHOA (AFU_ORTHOLOGUE AFUA_1G03570); 1.
DR PANTHER; PTHR31956; NON-SPECIFIC PHOSPHOLIPASE C4-RELATED; 1.
DR Pfam; PF04185; Phosphoesterase; 1.
DR Pfam; PF05506; PLipase_C_C; 2.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Hydrolase; Reference proteome; Signal.
FT SIGNAL 1..35
FT /note="Tat-type signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00648"
FT CHAIN 36..692
FT /note="Non-hemolytic phospholipase C"
FT /id="PRO_0000023941"
FT CONFLICT 18
FT /note="G -> A (in Ref. 1; AAA25968/AAA25969)"
FT /evidence="ECO:0000305"
FT CONFLICT 385
FT /note="P -> A (in Ref. 1; AAA25968/AAA25969)"
FT /evidence="ECO:0000305"
FT CONFLICT 584..585
FT /note="ER -> DG (in Ref. 1; AAA25968/AAA25969)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 692 AA; 77145 MW; 6B16C0BEEB25CAEF CRC64;
MISKSRRSFI RLAAGTVGAT VATSMLPSSI QAALAIPAHR RHGNLKDVEH VVILMQENRS
FDHYFGTLKG VRGFGDRMAI PLPDGQRVWH QKGSKGEILP YHFDTSTTSA QRVDGTPHTW
PDAQQAWNEG RMDKWLPAKT ERSLGYYKEQ DIAFQFAMAN AFTICDAYHC SFQGGTNPNR
LFLWTGTNDP LGQHGGPVTT NDHDSNGPVE QGYTWTTYPE RLQAAGITWR VYQDMADNFS
DNPLIGFRQY RAAAPDSPLI VNGLSTWKLD ALKRDVLANS LPQVSWIVAP AKYSEHPGPS
SPIWGAEYTS WVLDALTANP EVWSKTALLV MFDENDGFFD HVAPPAAPSL NKDGTLRGKT
TADATLEWHT KGDIRYRNQP YGLGPRVPMY VISPWSKGGW VNSQVFDHTS VIRFLEQRFG
VMEPNISPWR RAVCGDLTSA FNFANPNNEP FPELPDTSQA DAIVASQIKL PKPKPPAVAA
MPKQEMGIRP ARALPYELGV HARYRSGGDA LSLTFANTGK AGAVFQVFDL LDSENPPKRY
TVGARKRLHD SFQGDASGDY HLEVHGPNGF LRVFRGNLRR DLAERKAPLP EVRIDYEPLF
GNLRVQLINR GRHPVKLTVK DNVYRQGERR TVNVPPGQRR EVRYSLRSSG NWYDFSVSAQ
GADSFLRRFS GRMEDGRSGF SDPGMGLGTL TF
//
