ID PRPR_ECOLI Reviewed; 528 AA.
AC P77743; Q2MC93;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 27-MAR-2024, entry version 175.
DE RecName: Full=Propionate catabolism operon regulatory protein;
GN Name=prpR; Synonyms=yahP; OrderedLocusNames=b0330, JW0322;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Involved in the transcriptional regulation of the propionate
CC catabolism operon.
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DR EMBL; U73857; AAB18054.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73433.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76113.1; -; Genomic_DNA.
DR PIR; B64760; B64760.
DR RefSeq; NP_414864.1; NC_000913.3.
DR RefSeq; WP_000941041.1; NZ_SSZK01000063.1.
DR PDB; 2PJU; X-ray; 2.10 A; A/B/C/D=2-215.
DR PDBsum; 2PJU; -.
DR AlphaFoldDB; P77743; -.
DR SMR; P77743; -.
DR BioGRID; 4259807; 138.
DR DIP; DIP-10582N; -.
DR IntAct; P77743; 1.
DR STRING; 511145.b0330; -.
DR jPOST; P77743; -.
DR PaxDb; 511145-b0330; -.
DR EnsemblBacteria; AAC73433; AAC73433; b0330.
DR GeneID; 944987; -.
DR KEGG; ecj:JW0322; -.
DR KEGG; eco:b0330; -.
DR PATRIC; fig|1411691.4.peg.1946; -.
DR EchoBASE; EB3369; -.
DR eggNOG; COG3829; Bacteria.
DR HOGENOM; CLU_000445_8_5_6; -.
DR InParanoid; P77743; -.
DR OMA; IMLYARS; -.
DR OrthoDB; 9804019at2; -.
DR PhylomeDB; P77743; -.
DR BioCyc; EcoCyc:G6195-MONOMER; -.
DR EvolutionaryTrace; P77743; -.
DR PRO; PR:P77743; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; IDA:EcoCyc.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:EcoCyc.
DR GO; GO:0019629; P:propionate catabolic process, 2-methylcitrate cycle; IEA:InterPro.
DR GO; GO:0009314; P:response to radiation; IMP:EcoCyc.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 1.20.5.170; -; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR012704; Sig_transdc_resp-reg_PrpR.
DR InterPro; IPR010524; Sig_transdc_resp-reg_PrpR_N.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR NCBIfam; TIGR02329; propionate_PrpR; 1.
DR PANTHER; PTHR32071:SF21; PROPIONATE CATABOLISM OPERON REGULATORY PROTEIN; 1.
DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF06506; PrpR_N; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF159800; PrpR receptor domain-like; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA-binding; Nucleotide-binding;
KW Reference proteome; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..528
FT /note="Propionate catabolism operon regulatory protein"
FT /id="PRO_0000081324"
FT DOMAIN 218..461
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 508..527
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT BINDING 318..327
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT STRAND 13..17
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 20..30
FT /evidence="ECO:0007829|PDB:2PJU"
FT TURN 31..36
FT /evidence="ECO:0007829|PDB:2PJU"
FT STRAND 38..42
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 46..56
FT /evidence="ECO:0007829|PDB:2PJU"
FT TURN 57..59
FT /evidence="ECO:0007829|PDB:2PJU"
FT STRAND 63..68
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 69..76
FT /evidence="ECO:0007829|PDB:2PJU"
FT STRAND 83..86
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 90..99
FT /evidence="ECO:0007829|PDB:2PJU"
FT STRAND 107..114
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 117..126
FT /evidence="ECO:0007829|PDB:2PJU"
FT STRAND 130..137
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 138..150
FT /evidence="ECO:0007829|PDB:2PJU"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 160..168
FT /evidence="ECO:0007829|PDB:2PJU"
FT STRAND 171..177
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 179..195
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 199..204
FT /evidence="ECO:0007829|PDB:2PJU"
FT HELIX 212..215
FT /evidence="ECO:0007829|PDB:2PJU"
SQ SEQUENCE 528 AA; 58649 MW; 8EAFD1F55D96E8FF CRC64;
MAHPPRLNDD KPVIWTVSVT RLFELFRDIS LEFDHLANIT PIQLGFEKAV TYIRKKLANE
RCDAIIAAGS NGAYLKSRLS VPVILIKPSG YDVLQALAKA GKLTSSIGVV TYQETIPALV
AFQKTFNLRL DQRSYITEED ARGQINELKA NGTEAVVGAG LITDLAEEAG MTGIFIYSAA
TVRQAFSDAL DMTRMSLRHN THDATRNALR TRYVLGDMLG QSPQMEQVRQ TILLYARSSA
AVLIEGETGT GKELAAQAIH REYFARHDAR QGKKSHPFVA VNCGAIAESL LEAELFGYEE
GAFTGSRRGG RAGLFEIAHG GTLFLDEIGE MPLPLQTRLL RVLEEKEVTR VGGHQPVPVD
VRVISATHCN LEEDMQQGRF RRDLFYRLSI LRLQLPPLRE RVADILPLAE SFLKVSLAAL
SAPFSAALRQ GLQASETVLL HYDWPGNIRE LRNMMERLAL FLSVEPTPDL TPQFMQLLLP
ELARESAKTP APRLLTPQQA LEKFNGDKTA AANYLGISRT TFWRRLKS
//
