ID Q0A7Y9_ALKEH Unreviewed; 904 AA.
AC Q0A7Y9;
DT 17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 17-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 104.
DE SubName: Full=Assimilatory nitrate reductase (NADH) alpha subunit apoprotein {ECO:0000313|EMBL:ABI57048.1};
DE EC=1.7.1.1 {ECO:0000313|EMBL:ABI57048.1};
GN OrderedLocusNames=Mlg_1702 {ECO:0000313|EMBL:ABI57048.1};
OS Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Ectothiorhodospiraceae; Alkalilimnicola.
OX NCBI_TaxID=187272 {ECO:0000313|EMBL:ABI57048.1, ECO:0000313|Proteomes:UP000001962};
RN [1] {ECO:0000313|Proteomes:UP000001962}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1101 / DSM 17681 / MLHE-1
RC {ECO:0000313|Proteomes:UP000001962};
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S.,
RA Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B.,
RA Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P.;
RT "Complete sequence of Alkalilimnicola ehrilichei MLHE-1.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
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DR EMBL; CP000453; ABI57048.1; -; Genomic_DNA.
DR RefSeq; WP_011629442.1; NC_008340.1.
DR AlphaFoldDB; Q0A7Y9; -.
DR KEGG; aeh:Mlg_1702; -.
DR eggNOG; COG0243; Bacteria.
DR eggNOG; COG1251; Bacteria.
DR HOGENOM; CLU_000422_13_4_6; -.
DR OrthoDB; 9810782at2; -.
DR Proteomes; UP000001962; Chromosome.
DR GO; GO:1990204; C:oxidoreductase complex; IEA:UniProt.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0009703; F:nitrate reductase (NADH) activity; IEA:UniProtKB-EC.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:ABI57048.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001962}.
FT DOMAIN 11..67
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 904 AA; 97995 MW; 96B32D8535B0F499 CRC64;
MNTSIQPPRQ PGEVHTTCPY CGVGCGVLAR MDKDNRVHVR GDPEHPANFG RLCSKGAALG
ETTGLNGRLL HPRVHGQRTD WDTALATVAD GLNRVIRRDG PEAVGFYVSG QLLTEDYYVA
NKLMKGYIGS ANIDTNSRLC MASSVAGHIR AFGEDLVPGC YEDLERADLV VLTGSNLAWC
HPVLYQRLEA ARARRPGMRV VVIDPRETET AAQADLHLPL RPGSDVALWN GLFRRLVETD
QIHRDIRPET EAADSNLKRI LHEAGDADAV ASACDLNPRA LHLFYDWFAG TERTVSVYSQ
GVNQSSQGTD KVNAIINVHI LTGRLGRPGC GPFSVTGQPN AMGGREVGGL SNQLAAHMGF
SQGDRIRVQR YWDSPRIARQ PGMKAVELFR AAAQGRVKAL WIMATNPVVS LPDADLVKRA
LARCELVVVS DCIANTDTTG YAHVLMPAAA WGEKSGTVTN SERCISRQRA FRTPAGEARP
DWWIISQVAR LMGFDRGFAY GSPREIFDEH ARLTAVHNPG PGMGGRTLHL GALAGMNAKQ
WDTLQPVQWP CPGPGLDTAG PGRGTQRLAT NGGLPTEDGH PRLHPVCAET PGNTPDARFP
LVLNTGRTRD HWHTLTRTGL SVSLSTHQPE PRCDLHPEDA TRFGLADGHL VRVRSAWGSV
LLRARYQTGQ RRGELFVPMH WNDCYAAQAR IGAVANPITD PISGQPELKH TPVAVEAVPA
AAYGFVLSRA DDLPPPETAY WVRINGHAHQ RFVFASTEAP DSWRDWAGRW LGTEGTVVEM
ADRARGVYRF ARLVDDRLVA CLFIAADPAA LPGCDWLAGL LDNNRPLGAE ERLALLAGRP
AGEAETGETV CACFGVGERI IESAVAAGAH DTEAVTRHCK AGGYCGSCRP AINAIIQRLA
RSAA
//
