ID Q0K1V4_CUPNH Unreviewed; 839 AA.
AC Q0K1V4;
DT 03-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 100.
DE RecName: Full=Glucans biosynthesis glucosyltransferase H {ECO:0000256|ARBA:ARBA00020585, ECO:0000256|HAMAP-Rule:MF_01072};
DE EC=2.4.1.- {ECO:0000256|HAMAP-Rule:MF_01072};
GN Name=mdoH {ECO:0000313|EMBL:CAJ96020.1};
GN Synonyms=opgH {ECO:0000256|HAMAP-Rule:MF_01072};
GN OrderedLocusNames=H16_B1230 {ECO:0000313|EMBL:CAJ96020.1};
OS Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS / H16 / Stanier 337) (Ralstonia eutropha).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=381666 {ECO:0000313|EMBL:CAJ96020.1, ECO:0000313|Proteomes:UP000008210};
RN [1] {ECO:0000313|EMBL:CAJ96020.1, ECO:0000313|Proteomes:UP000008210}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier
RC 337 {ECO:0000313|Proteomes:UP000008210};
RX PubMed=16964242; DOI=10.1038/nbt1244;
RA Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA Eitinger T., Ewering C., Potter M., Schwartz E., Strittmatter A., Voss I.,
RA Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT eutropha H16.";
RL Nat. Biotechnol. 24:1257-1262(2006).
CC -!- FUNCTION: Involved in the biosynthesis of osmoregulated periplasmic
CC glucans (OPGs). {ECO:0000256|HAMAP-Rule:MF_01072}.
CC -!- PATHWAY: Glycan metabolism; osmoregulated periplasmic glucan (OPG)
CC biosynthesis. {ECO:0000256|ARBA:ARBA00005001, ECO:0000256|HAMAP-
CC Rule:MF_01072}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_01072}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_01072}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass
CC membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. OpgH
CC subfamily. {ECO:0000256|ARBA:ARBA00009337, ECO:0000256|HAMAP-
CC Rule:MF_01072}.
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DR EMBL; AM260480; CAJ96020.1; -; Genomic_DNA.
DR AlphaFoldDB; Q0K1V4; -.
DR STRING; 381666.H16_B1230; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR KEGG; reh:H16_B1230; -.
DR eggNOG; COG2943; Bacteria.
DR HOGENOM; CLU_015730_0_0_4; -.
DR UniPathway; UPA00637; -.
DR Proteomes; UP000008210; Chromosome 2.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016758; F:hexosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009250; P:glucan biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd04191; Glucan_BSP_MdoH; 1.
DR HAMAP; MF_01072; MdoH_OpgH; 1.
DR InterPro; IPR023725; Glucans_biosynth_gluTrFase_H.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR43867; CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]; 1.
DR PANTHER; PTHR43867:SF5; GLUCANS BIOSYNTHESIS GLUCOSYLTRANSFERASE H; 1.
DR Pfam; PF13632; Glyco_trans_2_3; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519, ECO:0000256|HAMAP-
KW Rule:MF_01072}; Cell membrane {ECO:0000256|HAMAP-Rule:MF_01072};
KW Glycosyltransferase {ECO:0000256|HAMAP-Rule:MF_01072};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_01072};
KW Reference proteome {ECO:0000313|Proteomes:UP000008210};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01072};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_01072};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_01072}.
FT TRANSMEM 158..179
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01072"
FT TRANSMEM 191..217
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01072"
FT TRANSMEM 509..536
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01072"
FT TRANSMEM 564..587
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01072"
FT TRANSMEM 599..620
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01072"
FT TRANSMEM 676..697
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01072"
FT DOMAIN 339..533
FT /note="Glycosyltransferase 2-like"
FT /evidence="ECO:0000259|Pfam:PF13632"
SQ SEQUENCE 839 AA; 93172 MW; D60BCA483BB20C31 CRC64;
MRSELLADGA PATPVAPVTP VASVAPVIAV TADGRDAQDA ITRLQSRLAA KDTPPAQGGS
AYASVGRRFT IAYGNPQVDG APLLQRREDG TVKVDTGPEP ERSSMVPRQW PPHIVTGWLR
NTWRRMLGRP PVPETWDTLH DGPDAEGKWH PAGSHRRWFL LGLVVIQTVL ATYFMSNVLP
YHGADPLEIA ILVLFAVLFS WVSAGFWTAM MGFLVLAKGG DRHLISRSAA PDGPIAPEAR
TAIIMPICNE DVTRVFAGLR ATYESLARTS HLSNFDFIVL SDSGNPDLRT AEHDAWMELC
RAVGGFGRIF YRWRRHRVKR KTGNVADFCR RWGSKYRYMV VLDADSVMSG DCLATLVRLM
EANPGAGIIQ SAPLAVGRET LYARVQQFAT RVYGPLFTAG LHYWQLGESH YWGHNAIIRV
HPFIEHCALA PLPGRGPLSG EILSHDFVEA ALMRRAGWGV WIAYDLEGSY EELPPNLLDE
VKRDRRWCQG NLMNFRLWLK QGFHVVHRAV FLTGIMAYLS APLWLLFLLL STAMLAKHAL
VPPEYFTQQY QLFPTWPEWH PEKALALFSA TATLLFLPKL ASVVLLLKQA RRYGGAVQLI
ASMLIEVMLS ALLAPTRMLF HTKFVIAAYS GWGISWKSPP REDAETSWGE AFRRHGWHTA
LGLAWGGLVY WLNPSYVLWL LPIVGSLALS IPLSVMLSRV SFGRASRNAG LFMIPEETQV
PREIVETQQH VEAAAETPDF VDAVVDPVTN ALMCATATPR VVQPESARKR HEALVEHALT
HGPRALTPAQ KHVLLGNPFA LARLHELVWG SPLADAGWKD TRILVRRAAN VLPLRPRAA
//
