ID Q17HK7_AEDAE Unreviewed; 812 AA.
AC Q17HK7;
DT 25-JUL-2006, integrated into UniProtKB/TrEMBL.
DT 25-JUL-2006, sequence version 1.
DT 27-MAR-2024, entry version 102.
DE RecName: Full=alpha-1,2-Mannosidase {ECO:0000256|RuleBase:RU361193};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361193};
GN Name=5575448 {ECO:0000313|EnsemblMetazoa:AAEL002645-PA};
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159 {ECO:0000313|EnsemblMetazoa:AAEL002645-PA, ECO:0000313|Proteomes:UP000008820};
RN [1] {ECO:0000313|EnsemblMetazoa:AAEL002645-PA, ECO:0000313|Proteomes:UP000008820}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVP_AGWG {ECO:0000313|EnsemblMetazoa:AAEL002645-PA,
RC ECO:0000313|Proteomes:UP000008820};
RG Aedes aegypti Genome Working Group (AGWG);
RA Matthews B.J.;
RT "Aedes aegypti genome working group (AGWG) sequencing and assembly.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:AAEL002645-PA}
RP IDENTIFICATION.
RC STRAIN=LVP_AGWG {ECO:0000313|EnsemblMetazoa:AAEL002645-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR601382-2};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 47 family.
CC {ECO:0000256|ARBA:ARBA00007658, ECO:0000256|RuleBase:RU361193}.
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DR RefSeq; XP_001661964.1; XM_001661914.1.
DR AlphaFoldDB; Q17HK7; -.
DR PaxDb; 7159-AAEL002645-PA; -.
DR EnsemblMetazoa; AAEL002645-RA; AAEL002645-PA; AAEL002645.
DR GeneID; 5575448; -.
DR KEGG; aag:5575448; -.
DR VEuPathDB; VectorBase:AAEL002645; -.
DR eggNOG; KOG2430; Eukaryota.
DR HOGENOM; CLU_003818_4_1_1; -.
DR InParanoid; Q17HK7; -.
DR OMA; NYVCANP; -.
DR OrthoDB; 942598at2759; -.
DR Proteomes; UP000008820; Chromosome 2.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd02126; PA_EDEM3_like; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR044674; EDEM1/2/3.
DR InterPro; IPR037322; EDEM3_PA.
DR InterPro; IPR001382; Glyco_hydro_47.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR036026; Seven-hairpin_glycosidases.
DR PANTHER; PTHR45679; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2; 1.
DR PANTHER; PTHR45679:SF2; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3; 1.
DR Pfam; PF01532; Glyco_hydro_47; 1.
DR Pfam; PF02225; PA; 1.
DR PRINTS; PR00747; GLYHDRLASE47.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF48225; Seven-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR601382-2};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycosidase {ECO:0000256|RuleBase:RU361193};
KW Hydrolase {ECO:0000256|RuleBase:RU361193};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601382-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000008820}.
FT DOMAIN 661..743
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT ACT_SITE 141
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 286
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 380
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 398
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT BINDING 484
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-2"
SQ SEQUENCE 812 AA; 90969 MW; 03F55F6D0B94F4BD CRC64;
MRTLSGSWCF LLHYVALAVV LSNCDAKADH IPSVPTHNMS HKERNELKEE AREMFYHAYG
AYMEKAYPAD ELMPLSCTGR YRGITPSRGD LDDVLGNFSM TLVDTLDTLV ILGDLEEFER
AVKLVIKDVK FDSDIIVSVF ETNIRMVGGL LSAHILAEYV QKQADVMTWY RGELLEMAKD
LGYRLLPAFN TSTGIPHARV NLKHGMKVEA LRHSRETCTA CAGTILLEFA ALSRLSGEPI
FEAKAHAAMD ALWKMRHRSS DLMGTVLNVH SGDWIRRESG VGAGIDSYYE YCLKSYILLG
DERYLARFNR HYNAIMKYIS QGPMLLDVQM HRPHTKTRNF MDALLAFWPG LQVLSGDLKP
AVQTHEMLYQ VMQMHTFIPE AFTYDFQVHW GQHHLRPEFI ESTYFLYRAT GDHYYLQAGK
KALKALQKHA KVPCGYAAVN DVRTGKHEDR MDSYVLSETF KYLYLLFSDP SDLLLNIENF
IFTTEAHLLP LTLGQLGNHT FNIKEHEEGN MLDFMRSCPS PNKLFPETVR RPLRDLVTGV
CPRISSAKRL RAADFQASNA DHLRTVYDMG ITMVSLGEGK VQLLHSFFNA KSPEDAERGL
IFMQEMVELS KSNAIPKTQL QEVSFQRSGE ELQVLKAGPS HFGPELTGDM SISQRAVFAS
PSKVCTSLKN SRELKGRIAI LERGDCTFVD KARRVQAAGS VAAIVYDNTP NTSIDNQQMF
AMSGDGQDDV KIPVVFLFTR EAEVLLAAVK ADPKLEITLR AVKELSDLHS SSSSGAAAKA
YSQKTETKTE SAQTSSSSSS SSSSSESKSN KP
//
