ID Q183M8_CLOD6 Unreviewed; 719 AA.
AC Q183M8;
DT 25-JUL-2006, integrated into UniProtKB/TrEMBL.
DT 25-JUL-2006, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE SubName: Full=Precursor of the S-layer proteins {ECO:0000313|EMBL:CAJ69681.1};
GN Name=slpA {ECO:0000313|EMBL:CAJ69681.1};
GN OrderedLocusNames=CD630_27930 {ECO:0000313|EMBL:CAJ69681.1};
OS Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC Clostridioides.
OX NCBI_TaxID=272563 {ECO:0000313|EMBL:CAJ69681.1, ECO:0000313|Proteomes:UP000001978};
RN [1] {ECO:0000313|EMBL:CAJ69681.1, ECO:0000313|Proteomes:UP000001978}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=630 {ECO:0000313|EMBL:CAJ69681.1,
RC ECO:0000313|Proteomes:UP000001978};
RX PubMed=16804543; DOI=10.1038/ng1830;
RA Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA Price C., Rabbinowitsch R., Sharp S., Simmonds M., Steven K., Unwin L.,
RA Whithead S., Dupuy B., Dougan G., Barrell B.and.Parkhill.J.;
RT "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT mobile, mosaic genome.";
RL Nat. Genet. 38:779-786(2006).
RN [2] {ECO:0007829|PDB:3CVZ}
RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 25-286.
RX PubMed=19183279; DOI=10.1111/j.1365-2958.2009.06603.x;
RA Fagan R.P., Albesa-Jove D., Qazi O., Svergun D.I., Brown K.A.,
RA Fairweather N.F.;
RT "Structural insights into the molecular organization of the S-layer from
RT Clostridium difficile.";
RL Mol. Microbiol. 71:1308-1322(2009).
RN [3] {ECO:0007829|PDB:7ACY, ECO:0007829|PDB:7QGQ}
RP STRUCTURE BY NMR OF 25-342 AND 347-719.
RX PubMed=35217634; DOI=10.1038/s41467-022-28196-w;
RA Lanzoni-Mangutchi P., Banerji O., Wilson J., Barwinska-Sendra A.,
RA Kirk J.A., Vaz F., O'Beirne S., Basle A., El Omari K., Wagner A.,
RA Fairweather N.F., Douce G.R., Bullough P.A., Fagan R.P., Salgado P.S.;
RT "Structure and assembly of the S-layer in C. difficile.";
RL Nat. Commun. 13:970-970(2022).
CC -!- INTERACTION:
CC Q183M8; Q183M8: slpA; NbExp=4; IntAct=EBI-6478332, EBI-6478332;
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DR EMBL; AM180355; CAJ69681.1; -; Genomic_DNA.
DR RefSeq; WP_011861686.1; NZ_JAUPES010000010.1.
DR RefSeq; YP_001089306.1; NC_009089.1.
DR PDB; 3CVZ; X-ray; 2.40 A; A/B/C/D=25-286.
DR PDB; 7ACY; X-ray; 2.55 A; A/C=25-342, B/D=347-719.
DR PDB; 7QGQ; EM; -; A/C/E/F/G/H/I/O/P/Q/R/S=25-342, B/D/J/K/L/M/N/T/U/V/W/X=347-719.
DR PDBsum; 3CVZ; -.
DR AlphaFoldDB; Q183M8; -.
DR EMDB; EMD-13957; -.
DR SMR; Q183M8; -.
DR STRING; 272563.CD630_27930; -.
DR ChEMBL; CHEMBL1938206; -.
DR EnsemblBacteria; CAJ69681; CAJ69681; CD630_27930.
DR KEGG; cdf:CD630_27930; -.
DR KEGG; pdc:CDIF630_03056; -.
DR PATRIC; fig|272563.120.peg.2941; -.
DR eggNOG; COG2247; Bacteria.
DR OrthoDB; 1744233at2; -.
DR PhylomeDB; Q183M8; -.
DR BioCyc; PDIF272563:G12WB-2952-MONOMER; -.
DR EvolutionaryTrace; Q183M8; -.
DR Proteomes; UP000001978; Chromosome.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR Gene3D; 3.40.50.12090; -; 2.
DR Gene3D; 3.30.1490.290; Low molecular weight S-layer protein, domain 1; 1.
DR InterPro; IPR007253; Cell_wall-bd_2.
DR InterPro; IPR021071; Low_molc_weight_S-layer_N.
DR InterPro; IPR043090; Low_molc_weight_S-layer_N_sub.
DR NCBIfam; NF033435; S-layer_Clost; 1.
DR PANTHER; PTHR30032:SF8; AMIDASE ENHANCER; 1.
DR PANTHER; PTHR30032; N-ACETYLMURAMOYL-L-ALANINE AMIDASE-RELATED; 1.
DR Pfam; PF04122; CW_binding_2; 3.
DR Pfam; PF12211; LMWSLP_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3CVZ, ECO:0007829|PDB:7ACY};
KW Reference proteome {ECO:0000313|Proteomes:UP000001978};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..719
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004186821"
FT DOMAIN 29..290
FT /note="Low molecular weight S layer protein N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12211"
SQ SEQUENCE 719 AA; 76133 MW; DBE67B738C2FAA20 CRC64;
MNKKNIAIAM SGLTVLASAA PVFAATTGTQ GYTVVKNDWK KAVKQLQDGL KDNSIGKITV
SFNDGVVGEV APKSANKKAD RDAAAEKLYN LVNTQLDKLG DGDYVDFSVD YNLENKIITN
QADAEAIVTK LNSLNEKTLI DIATKDTFGM VSKTQDSEGK NVAATKALKV KDVATFGLKS
GGSEDTGYVV EMKAGAVEDK YGKVGDSTAG IAINLPSTGL EYAGKGTTID FNKTLKVDVT
GGSTPSAVAV SGFVTKDDTD LAKSGTINVR VINAKEESID IDASSYTSAE NLAKRYVFDP
DEISEAYKAI VALQNDGIES NLVQLVNGKY QVIFYPEGKR LETKSANDTI ASQDTPAKVV
IKANKLKDLK DYVDDLKTYN NTYSNVVTVA GEDRIETAIE LSSKYYNSDD KNAITDKAVN
DIVLVGSTSI VDGLVASPLA SEKTAPLLLT SKDKLDSSVK SEIKRVMNLK SDTGINTSKK
VYLAGGVNSI SKDVENELKN MGLKVTRLSG EDRYETSLAI ADEIGLDNDK AFVVGGTGLA
DAMSIAPVAS QLKDGDATPI VVVDGKAKEI SDDAKSFLGT SDVDIIGGKN SVSKEIEESI
DSATGKTPDR ISGDDRQATN AEVLKEDDYF TDGEVVNYFV AKDGSTKEDQ LVDALAAAPI
AGRFKESPAP IILATDTLSS DQNVAVSKAV PKDGGTNLVQ VGKGIASSVI NKMKDLLDM
//
