ID Q194Q9_STRAA Unreviewed; 514 AA.
AC Q194Q9;
DT 11-JUL-2006, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2006, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE SubName: Full=Acyl CoA ligase {ECO:0000313|EMBL:CAK50779.1};
GN Name=mtmL {ECO:0000313|EMBL:CAK50779.1};
OS Streptomyces argillaceus.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=41951 {ECO:0000313|EMBL:CAK50779.1};
RN [1] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=8807845; DOI=10.1016/S1074-5521(96)90262-8;
RA Blanco G., Fu H., Mendez C., Khosla C., Salas J.A.;
RT "Deciphering the biosynthetic origin of the aglycone of the aureolic acid
RT group of anti-tumor agents.";
RL Chem. Biol. 3:193-196(1996).
RN [2] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=8654997; DOI=10.1016/0378-1119(96)00029-7;
RA Lombo F., Blanco G., Fernandez E., Mendez C., Salas J.A.;
RT "Characterization of Streptomyces argillaceus genes encoding a polyketide
RT synthase involved in the biosynthesis of the antitumor mithramycin.";
RL Gene 172:87-91(1996).
RN [3] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=8757400; DOI=10.1007/s004380050218;
RA Fernandez E., Lombo F., Mendez C., Salas J.A.;
RT "An ABC transporter is essential for resistance to the antitumor agent
RT mithramycin in the producer Streptomyces argillaceus.";
RL Mol. Gen. Genet. 251:692-698(1996).
RN [4] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=9375253; DOI=10.1016/S1074-5521(97)90313-6;
RA Kantola J., Blanco G., Hautala A., Kunnari T., Hakala J., Mendez C.,
RA Ylihonko K., Mantsala P., Salas J.;
RT "Folding of the polyketide chain is not dictated by minimal polyketide
RT synthase in the biosynthesis of mithramycin and anthracycline.";
RL Chem. Biol. 4:751-755(1997).
RN [5] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=9150235;
RA Lombo F., Siems K., Brana A.F., Mendez C., Bindseil K., Salas J.A.;
RT "Cloning and insertional inactivation of Streptomyces argillaceus genes
RT involved in the earliest steps of biosynthesis of the sugar moieties of the
RT antitumor polyketide mithramycin.";
RL J. Bacteriol. 179:3354-3357(1997).
RN [6] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=9733697;
RA Fernandez E., Weissbach U., Reillo C.S., Brana A.F., Mendez C., Rohr J.,
RA Salas J.A.;
RT "Identification of two genes from Streptomyces argillaceus encoding
RT glycosyltransferases involved in transfer of a disaccharide during
RT biosynthesis of the antitumor drug mithramycin.";
RL J. Bacteriol. 180:4929-4937(1998).
RN [7] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=9889148; DOI=10.1016/S1074-5521(99)80017-9;
RA Prado L., Fernandez E., Weissbach U., Blanco G., Quiros L.M., Brana A.F.,
RA Mendez C., Rohr J., Salas J.A.;
RT "Oxidative cleavage of premithramycin B is one of the last steps in the
RT biosynthesis of the antitumor drug mithramycin.";
RL Chem. Biol. 6:19-30(1999).
RN [8] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=9917296; DOI=10.1021/np980355k;
RA Wohlert S.E., Kunzel E., Machinek R., Mendez C., Salas J.A., Rohr J.;
RT "The structure of mithramycin reinvestigated.";
RL J. Nat. Prod. 62:119-121(1999).
RN [9] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=10102355; DOI=10.1007/s004380050960;
RA Prado L., Lombo F., Brana A.F., Mendez C., Rohr J., Salas J.A.;
RT "Analysis of two chromosomal regions adjacent to genes for a type II
RT polyketide synthase involved in the biosynthesis of the antitumor
RT polyketide mithramycin in Streptomyces argillaceus.";
RL Mol. Gen. Genet. 261:216-225(1999).
RN [10] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=10760873;
RX DOI=10.1002/(SICI)1521-3773(20000218)39:4<796::AID-ANIE796>3.0.CO;2-N;
RA Lombo F., Kunzel E., Prado L., Brana A.F., Bindseil K.U., Frevert J.,
RA Bearden D., Mendez C., Salas J.A., Rohr J.;
RT "The Novel Hybrid Antitumor Compound Premithramycinone H Provides Indirect
RT Evidence for a Tricyclic Intermediate of the Biosynthesis of the Aureolic
RT Acid Antibiotic Mithramycin.";
RL Angew. Chem. Int. Ed. 39:796-799(2000).
RN [11] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=10779713; DOI=10.1111/j.1574-6968.2000.tb09082.x;
RA Garcia-Bernardo J., Brana A.F., Mendez C., Salas J.A.;
RT "Insertional inactivation of mtrX and mtrY genes from the mithramycin gene
RT cluster affects production and growth of the producer organism Streptomyces
RT argillaceus.";
RL FEMS Microbiol. Lett. 186:61-65(2000).
RN [12] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=10652287; DOI=10.1074/jbc.275.5.3065;
RA Lozano M.J., Remsing L.L., Quiros L.M., Brana A.F., Fernandez E.,
RA Sanchez C., Mendez C., Rohr J., Salas J.A.;
RT "Characterization of two polyketide methyltransferases involved in the
RT biosynthesis of the antitumor drug mithramycin by Streptomyces
RT argillaceus.";
RL J. Biol. Chem. 275:3065-3074(2000).
RN [13] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=10660060; DOI=10.1007/PL00008667;
RA Blanco G., Fernandez E., Fernandez M.J., Brana A.F., Weissbach U.,
RA Kunzel E., Rohr J., Mendez C., Salas J.A.;
RT "Characterization of two glycosyltransferases involved in early
RT glycosylation steps during biosynthesis of the antitumor polyketide
RT mithramycin by Streptomyces argillaceus.";
RL Mol. Gen. Genet. 262:991-1000(2000).
RN [14] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=11254130; DOI=10.1007/s004380000372;
RA Gonzalez A., Remsing L.L., Lombo F., Fernandez M.J., Prado L., Brana A.F.,
RA Kunzel E., Rohr J., Mendez C., Salas J.A.;
RT "The mtmVUC genes of the mithramycin gene cluster in Streptomyces
RT argillaceus are involved in the biosynthesis of the sugar moieties.";
RL Mol. Gen. Genet. 264:827-835(2001).
RN [15] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=11853433; DOI=10.1021/ja0105156;
RA Remsing L.L., Garcia-Bernardo J., Gonzalez A., Kunzel E., Rix U.,
RA Brana A.F., Bearden D.W., Mendez C., Salas J.A., Rohr J.;
RT "Ketopremithramycins and ketomithramycins, four new aureolic acid-type
RT compounds obtained upon inactivation of two genes involved in the
RT biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin
RT by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring
RT steps of the mithramycin biosynthetic pathway.";
RL J. Am. Chem. Soc. 124:1606-1614(2002).
RN [16] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=12022840; DOI=10.1021/ja017385l;
RA Trefzer A., Blanco G., Remsing L., Kunzel E., Rix U., Lipata F.,
RA Brana A.F., Mendez C., Rohr J., Bechthold A., Salas J.A.;
RT "Rationally designed glycosylated premithramycins: hybrid aromatic
RT polyketides using genes from three different biosynthetic pathways.";
RL J. Am. Chem. Soc. 124:6056-6062(2002).
RN [17] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=12733914; DOI=10.1021/ja034162h;
RA Remsing L.L., Gonzalez A.M., Nur-e-Alam M., Fernandez-Lozano M.J.,
RA Brana A.F., Rix U., Oliveira M.A., Mendez C., Salas J.A., Rohr J.;
RT "Mithramycin SK, a novel antitumor drug with improved therapeutic index,
RT mithramycin SA, and demycarosyl-mithramycin SK: three new products
RT generated in the mithramycin producer Streptomyces argillaceus through
RT combinatorial biosynthesis.";
RL J. Am. Chem. Soc. 125:5745-5753(2003).
RN [18] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=12813091; DOI=10.1128/JB.185.13.3962-3965.2003;
RA Rodriguez D., Quiros L.M., Brana A.F., Salas J.A.;
RT "Purification and characterization of a monooxygenase involved in the
RT biosynthetic pathway of the antitumor drug mithramycin.";
RL J. Bacteriol. 185:3962-3965(2003).
RN [19] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=14660589; DOI=10.1074/jbc.M312351200;
RA Rodriguez D., Quiros L.M., Salas J.A.;
RT "MtmMII-mediated C-methylation during biosynthesis of the antitumor drug
RT mithramycin is essential for biological activity and DNA-drug
RT interaction.";
RL J. Biol. Chem. 279:8149-8158(2004).
RN [20] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RX PubMed=15712316; DOI=10.1002/cbic.200400309;
RA Nur-e-Alam M., Mendez C., Salas J.A., Rohr J.;
RT "Elucidation of the glycosylation sequence of mithramycin biosynthesis:
RT isolation of 3A-deolivosylpremithramycin B and its conversion to
RT premithramycin B by glycosyltransferase MtmGII.";
RL ChemBioChem 6:632-636(2005).
RN [21] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RA Lombo F.;
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [22] {ECO:0000313|EMBL:CAK50779.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 12956 {ECO:0000313|EMBL:CAK50779.1};
RA Rohr J.;
RL Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; X89899; CAK50779.1; -; Genomic_DNA.
DR AlphaFoldDB; Q194Q9; -.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR PANTHER; PTHR43767; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR PANTHER; PTHR43767:SF1; NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 4: Predicted;
KW Ligase {ECO:0000313|EMBL:CAK50779.1}.
FT DOMAIN 19..378
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 427..502
FT /note="AMP-binding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13193"
FT REGION 324..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 514 AA; 54171 MW; A88F7539276BBBAD CRC64;
MSELPPGPAV PLDGLLRLAA ERHPEQVAVR DERGAVDYAG LDARADRFAR ALLRLTGGRP
TAVGVASVLD PVFAAAFYGT SRSGNRVVLV NPLVREPVLE HVFRTAGIEI ALVSAETRSP
GGRRAALPDL REVYVVDADR TGPPPPGTRP LDDLLTEDGT AALPDPAGVD LDSVVCVQFT
SGTTGPPKGV RLTHRNLVAN AAQAAHALGL DAGSVCLNHL PLYHVMHLDS AVYAGATQVL
CHDPDPVASV AAAAEAGATH YFGLPVRLAR LAADPRLASV VPGPELGLVR SGGSRLAPAV
AARLRERLGV PVIQGYGLAE LSPLSHNDRP ERSKPGSVGP AVPGTECRIV DLETGAALDP
GRPGEVLLRG PQLMAGYLGL PDAPDIDAAG WFHTGDVGYQ DEDGWLFLVD RIKDVFKVDN
ELVSPSEIEQ VLLQDPDVAD CVVADLPDEF SGAVVWAGVV PAGDGPVDLN PIVARANALL
SDHQRIRRAE RLTAVPRSPN GKTERTRLRE RLRA
//
