ID Q19RB6_9BACL Unreviewed; 493 AA.
AC Q19RB6;
DT 11-JUL-2006, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2006, sequence version 1.
DT 27-MAR-2024, entry version 83.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:ABF82591.1};
OS Exiguobacterium sp. Indian Stream.
OC Bacteria; Bacillota; Bacilli; Bacillales;
OC Bacillales Family XII. Incertae Sedis; Exiguobacterium.
OX NCBI_TaxID=383718 {ECO:0000313|EMBL:ABF82591.1};
RN [1] {ECO:0000313|EMBL:ABF82591.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Indian Stream {ECO:0000313|EMBL:ABF82591.1};
RX PubMed=17156079; DOI=10.1111/j.1574-6941.2006.00233.x;
RA Rodrigues D.F., Tiedje J.M.;
RT "Multi-locus real-time PCR for quantitation of bacteria in the environment
RT reveals Exiguobacterium to be prevalent in permafrost.";
RL FEMS Microbiol. Ecol. 59:489-499(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; DQ490866; ABF82591.1; -; Genomic_DNA.
DR AlphaFoldDB; Q19RB6; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd16928; HATPase_GyrB-like; 1.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:ABF82591.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 396..493
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ABF82591.1"
FT NON_TER 493
FT /evidence="ECO:0000313|EMBL:ABF82591.1"
SQ SEQUENCE 493 AA; 54260 MW; 08F67AD7D7C3BC4A CRC64;
GMYIGSTASK GLHHLVWEIV DNSIDEALAG YCDTIKVTIE PGNSILVEDN GRGIPVDIQE
KMGRPAVEVI LTVLHAGGKF GGGGYKVSGG LHGVGASVVN ALSTKLEVFV KRNEKLYYQA
YHRGVPVADL EIIGTSEETG TMIRFFPDDE IFQETLEYDY DLLATRLREL AFLNKGLTIE
ITDDRADEPI TRSFHYEGGI KSYVEHLNRS KEVVHEEPVY VHGNRDGIEV EVALQYNDGF
AANIYSFTNN IPTHEGGTHE TGFKTALTRV INDYAKKFGL MKEADGTLSG EDVREGMTAI
VSIKHPNPQF EGQTKTKLGN SDARTATDTL FSSAFEQFMM ENPTSARAIV EKGMMASRAR
MAAKRARELT RRKGVLEVSS LPGKLADCSS RDATISEIYI VEGDSAGGSA KSGRDRHFQA
ILPIRGKILN VEKARLDKIL GSNEIRTIIT ALGTSIGSEF NIEKARYHKV IIMTDADVDG
AHIRTLLLTF FYR
//
