ID Q1X6A7_BURVI Unreviewed; 603 AA.
AC Q1X6A7;
DT 02-MAY-2006, integrated into UniProtKB/TrEMBL.
DT 02-MAY-2006, sequence version 1.
DT 24-JAN-2024, entry version 86.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:AAY86031.1};
OS Burkholderia vietnamiensis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=60552 {ECO:0000313|EMBL:AAY86031.1};
RN [1] {ECO:0000313|EMBL:AAY86031.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FCF42 {ECO:0000313|EMBL:AAY86031.1};
RX PubMed=18312571; DOI=10.1111/j.1574-6968.2008.01105.x;
RA Tabacchioni S., Ferri L., Manno G., Mentasti M., Cocchi P., Campana S.,
RA Ravenni N., Taccetti G., Dalmastri C., Chiarini L., Bevivino A., Fani R.;
RT "Use of the gyrB gene to discriminate among species of the Burkholderia
RT cepacia complex.";
RL FEMS Microbiol. Lett. 281:175-182(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; AY996914; AAY86031.1; -; Genomic_DNA.
DR AlphaFoldDB; Q1X6A7; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR CDD; cd03366; TOPRIM_TopoIIA_GyrB; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.300.370; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR049353; GyrB_hook.
DR InterPro; IPR041423; GyrB_insert.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034160; TOPRIM_GyrB.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF21249; GyrB_hook; 1.
DR Pfam; PF18053; GyrB_insert; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 284..399
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AAY86031.1"
FT NON_TER 603
FT /evidence="ECO:0000313|EMBL:AAY86031.1"
SQ SEQUENCE 603 AA; 66955 MW; 22768E1BBFD4A36B CRC64;
HRGIAQDRVL EQVDGVEVSP MLVTGDTENR GTEVHFMADP TIFGTVEYHY DILAKRMREL
SFLNNGVRIR LTDLRSGKED DFAFAGGVKG FVEYINKTKT NLHPTVFFAN GEKDGVGVEV
AMQWNDSYNE NVLCFTNNIP QRDGGTHLTG LRAAMTRVIN KYITDNEIAK KAKVETTGDD
MREGLSCVLS VKVPEPKFSS QTKDKLVSSE VRAPVEEVVA KALEEFLLET PTDAKIICGK
IVEAARARDA ARKAREMTRR KGVLDGVGLP GKLADCQEKD PAKCEIYIVE GDSAGGSAKQ
GRDRKFQAIL PLRGKVLNVE KARYDKLLSS EQIVTLVTAL GCGIGKDDYN LDKLRYHRII
IMTDADVDGA HIRTLLLTFF YRQMPEMVER GYVYIAQPPL YKIKAGKDER YLKDDAELNA
HMLRLALQGS ELVPSENGAA ISGDALGELA RSYLLSQSVI GRLSRLYDPA ALEAIMDGVS
IDLSSEESTE ASARALHAAL HDETLKNEVR VVPSYDPVRE LRSLRVERAH HGNVRVSVID
EEFQHTADYQ QLVTTAKTFE GLIQAGAVIK RGERSMAVTD FKSAMKWLLA DAERNVSKQR
YKG
//
