ID Q27W60_STRVO Unreviewed; 1942 AA.
AC Q27W60;
DT 04-APR-2006, integrated into UniProtKB/TrEMBL.
DT 04-APR-2006, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE SubName: Full=NigAIX {ECO:0000313|EMBL:ABC84469.1};
OS Streptomyces violaceusniger.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces; Streptomyces violaceusniger group.
OX NCBI_TaxID=68280 {ECO:0000313|EMBL:ABC84469.1};
RN [1] {ECO:0000313|EMBL:ABC84469.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 4137 {ECO:0000313|EMBL:ABC84469.1};
RX PubMed=17584617; DOI=10.1016/j.chembiol.2007.05.011;
RA Harvey B.M., Mironenko T., Sun Y., Hong H., Deng Z., Leadlay P.F.,
RA Weissman K.J., Haydock S.F.;
RT "Insights into polyether biosynthesis from analysis of the nigericin
RT biosynthetic gene cluster in Streptomyces sp. DSM4137.";
RL Chem. Biol. 14:703-714(2007).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; DQ354110; ABC84469.1; -; Genomic_DNA.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:UniProt.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR CDD; cd08956; KR_3_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR036299; Polyketide_synth_docking_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 1.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 1.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF47336; ACP-like; 1.
DR SUPFAM; SSF101173; Docking domain B of the erythromycin polyketide synthase (DEBS); 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS50075; CARRIER; 1.
DR PROSITE; PS52004; KS3_2; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 33..459
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1766..1848
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1370..1390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1458..1487
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1465..1479
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1942 AA; 201709 MW; 63561AF5F94BF8D2 CRC64;
MTDDEKLRSY LKRATADLRL ARRQLREVED RAREPIAIIG IACHFPGDVA TPEDLWRVVD
EGVDVISSFP EDRGWDLDSL YDPDPEHAGT SSAREGGFLR DVADFDAEFF GISPREAAAM
DPQQRLLLET AWEAFERAGL TREALNGSRT GVFAGVDSYH YLSLIGQTTG DSAGYVATGN
LGSVVSGRVS YSFGLEGPAV TVDTACSSSL VATHLAVQAL RQDECSLALA GGVTVMATPG
GFTEFSRQRA LSPDGRCKAF AAAADGTGFS EGVGLVLLER LSDARRNGHR VLAVIRGSAV
NQDGASNGLT APNGPSQQRV IRQALANAGL SPSEVDAVEA HGTGTTLGDP IEAQALLATY
GQDRPEGRPL WLGSIKSNIG HTQAAAGVAS VIKMVQALRH EVLPVSLHID APTPHVDWGA
GEVRLLSEPV EWAANEQPRR AGVSSFGISG TNAHLILEQA PAQPVEAARA PDSSRGGVPA
GGVVPWVVSG RGAKALRGQA RALAERVATD PDASPAEVGW SLISTRSVFD HRAVIVGEHR
DELLAGLNAL ATGDTHPTLT EPTTTGPGAG GMGPVLVFPG QGSQWLGMGA GLLDTSPVFA
TRIAECEHAL TPHVDWSLTE VLRGGVNAAD LARVDVVQPV LWAVMISLAA VWDHHGLTPT
AVVGHSQGEI AAACVAGALS LDEGARIVAL RAHALRRLAG HGAMASLTTS QTHTEELLTN
LGEQADAVGV AAVNGPGSVV ISGPPEQVTH AITACEQAGH RARLIDVDYA SHSAQVDEIA
EELNQLLAGV KPVSAQVAFY STVTGARMDT SGLDTAYWIK NLRERVRFAD AVRALLHDGH
RVFIEASTHP VLTIGMQESI EEARVDAVTI PTLRRDHGGP DQLARSVARA FTAGLSVDWT
RWFPTDPAPR TIDLPTYAFQ RERYWLDGEG GPGGDPAGLG LTAAGHPLLG AAVELADGTT
HVLTGRISAR SHPWLAEHVV AGAVLAPGAV LVEWALRAAD AVGCGGVEEL ALQVPLALPE
SGGLRVQVVV GAAAEDGRRE VRMYSSPDHP NSLDPGLDSG ADTGWVCHAV GVLAPAAEAP
APVEGLGGAW PPPGAEPLDV GAFYEQVLAA GYGYGPAYQG LKAAWRQGGD VLAEVALPDA
AGGRDGYGIH PALLDAALHP SLLMDRPEGQ EDDGQVWLPF AWNGVSLWAT EAATVRVRLS
RDEERQSLRL TVADTVGAPV LTVDSLVTRP AAADQLCSAA AARAVDGLFT LDWMAVPAPA
TAPSPASVVG EGSWVVLGED RLGLAEPVAA GLGADATVSH PDLEALIAAV DAGEPAPAVV
LTHPYTAEGG HGDGLGAVEE LLGLVRSWLR RPALADARLV VVTRGAVAAG RAGDATDDAD
DTALDPSGVD PSGAGAWGLI RSAQSENPGR FVLLDLDEGH EGRAGLVEAV VRAVESHEPQ
MAVRDGRVLV PRMVRAMAAP GPTTDDRGES TTGLDENTTG LDGGATGLDT DGTVLITGGT
GTLGTLVAEH LVRAWGVRHL LLVSRSGPEA AGAGELTAAL TELGAEVRIA AVDVTDPDAV
AALVAGVDSA HPLTGVVHAA GVLDDGVLTT QTPERLARVW RPKATAAAHL HAATAELPLS
LFVMFSSTAG TLGASGQSNY AAANAYCDAL AARRHALGLP GLSVAWGLWA DASGMTGHLG
EADRARMTRS GIGAMSSERA LGLLDAAMRH GHHHLIAIDL DVRALTGRPA LTLPAPLRAL
AGGGTARRTA ATARPHTDWA GHLAALPLEE QRRTLLNLVL SHAAAALGHS DPGRIQTERG
FLELGFDSLT AIELRNRLTA VTGLRLATTL IFDHPNPAAL AAHLHSELAP EDADPLAPML
GEIDRMESAL LSVAQDETAH EALLERLRTT LSKLGALHGG GTEEGPAAGR IQDATADEIF
QFIDEDLGRS ESNGEHAHGG TR
//
