ID Q2FXP5_STAA8 Unreviewed; 306 AA.
AC Q2FXP5;
DT 21-MAR-2006, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2006, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE RecName: Full=Primosomal protein DnaI {ECO:0008006|Google:ProtNLM};
GN OrderedLocusNames=SAOUHSC_01791 {ECO:0000313|EMBL:ABD30859.1};
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061 {ECO:0000313|EMBL:ABD30859.1, ECO:0000313|Proteomes:UP000008816};
RN [1] {ECO:0000313|Proteomes:UP000008816}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47 {ECO:0000313|Proteomes:UP000008816};
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C
RL (2006).
RN [2] {ECO:0007829|PDB:5HE8}
RP X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 136-306.
RA Hood I.V., Berger J.M.;
RT "Bacterial initiation protein.";
RL Submitted (JAN-2016) to the PDB data bank.
RN [3] {ECO:0007829|PDB:5HE9}
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 136-306 IN COMPLEX WITH ADP AND
RP MG(2+).
RA Hood I.V., Berger J.M.;
RT "Structure of inhibited replicative helicase loader from Staphylococcus
RT aureus at 1.9 Angstrom resolution.";
RL Submitted (JAN-2016) to the PDB data bank.
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DR EMBL; CP000253; ABD30859.1; -; Genomic_DNA.
DR RefSeq; WP_000808621.1; NZ_LS483365.1.
DR RefSeq; YP_500295.1; NC_007795.1.
DR PDB; 5HE8; X-ray; 2.60 A; A/B/C/D/E/F/G/H/I/J/K/L=136-306.
DR PDB; 5HE9; X-ray; 1.90 A; A=136-306.
DR PDBsum; 5HE8; -.
DR PDBsum; 5HE9; -.
DR AlphaFoldDB; Q2FXP5; -.
DR SMR; Q2FXP5; -.
DR STRING; 93061.SAOUHSC_01791; -.
DR PaxDb; 1280-SAXN108_1710; -.
DR GeneID; 3920432; -.
DR KEGG; sao:SAOUHSC_01791; -.
DR PATRIC; fig|93061.5.peg.1631; -.
DR eggNOG; COG1484; Bacteria.
DR HOGENOM; CLU_077384_1_0_9; -.
DR OrthoDB; 61127at2; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR009928; DnaI_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR30050; CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA; 1.
DR PANTHER; PTHR30050:SF8; PRIMOSOMAL PROTEIN DNAI; 1.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF07319; DnaI_N; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:5HE8, ECO:0007829|PDB:5HE9};
KW Metal-binding {ECO:0007829|PDB:5HE9};
KW Nucleotide-binding {ECO:0007829|PDB:5HE9};
KW Reference proteome {ECO:0000313|Proteomes:UP000008816}.
FT DOMAIN 1..94
FT /note="Primosomal DnaI N-terminal"
FT /evidence="ECO:0000259|Pfam:PF07319"
FT DOMAIN 147..227
FT /note="Chromosomal replication initiator protein DnaA"
FT /evidence="ECO:0000259|Pfam:PF00308"
FT BINDING 167
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0007829|PDB:5HE9"
FT BINDING 168
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0007829|PDB:5HE9"
FT BINDING 169
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0007829|PDB:5HE9"
FT BINDING 170
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0007829|PDB:5HE9"
FT BINDING 171
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0007829|PDB:5HE9"
FT BINDING 171
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0007829|PDB:5HE9"
FT BINDING 172
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0007829|PDB:5HE9"
SQ SEQUENCE 306 AA; 35636 MW; 7F3440E89643505E CRC64;
MKQFKSIINT SQDFEKRIEK IKKEVINDPD VKQFLEAHRA ELTNAMIDED LNVLQEYKDQ
QKHYDGHKFA DCPNFVKGHV PELYVDNNRI KIRYLQCPCK IKYDEERFEA ELITSHHMQR
DTLNAKLKDI YMNHRDRLDV AMAADDICTA ITNGEQVKGL YLYGPFGTGK SFILGAIANQ
LKSKKVRSTI IYLPEFIRTL KGGFKDGSFE KKLHRVREAN ILMLDDIGAE EVTPWVRDEV
IGPLLHYRMV HELPTFFSSN FDYSELEHHL AMTRDGEEKT KAARIIERVK SLSTPYFLSG
ENFRNN
//
