ID Q2K7F2_RHIEC Unreviewed; 819 AA.
AC Q2K7F2;
DT 07-MAR-2006, integrated into UniProtKB/TrEMBL.
DT 07-MAR-2006, sequence version 1.
DT 27-MAR-2024, entry version 103.
DE RecName: Full=beta-mannosidase {ECO:0000256|ARBA:ARBA00012754};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
GN Name=manB2 {ECO:0000313|EMBL:ABC91234.1};
GN OrderedLocusNames=RHE_CH02457 {ECO:0000313|EMBL:ABC91234.1};
OS Rhizobium etli (strain CFN 42 / ATCC 51251).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=347834 {ECO:0000313|EMBL:ABC91234.1, ECO:0000313|Proteomes:UP000001936};
RN [1] {ECO:0000313|EMBL:ABC91234.1, ECO:0000313|Proteomes:UP000001936}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CFN 42 / ATCC 51251 {ECO:0000313|Proteomes:UP000001936};
RX PubMed=16505379; DOI=10.1073/pnas.0508502103;
RA Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.,
RA Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A.,
RA Jimenez-Jacinto V., Collado-Vides J., Davila G.;
RT "The partitioned Rhizobium etli genome: genetic and metabolic redundancy in
RT seven interacting replicons.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
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DR EMBL; CP000133; ABC91234.1; -; Genomic_DNA.
DR RefSeq; WP_011425713.1; NC_007761.1.
DR AlphaFoldDB; Q2K7F2; -.
DR CAZy; GH2; Glycoside Hydrolase Family 2.
DR KEGG; ret:RHE_CH02457; -.
DR eggNOG; COG3250; Bacteria.
DR HOGENOM; CLU_005015_3_2_5; -.
DR OrthoDB; 9758603at2; -.
DR Proteomes; UP000001936; Chromosome.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:ABC91234.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ABC91234.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001936}.
FT DOMAIN 195..291
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 739..802
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 819 AA; 92260 MW; 73A90F677B30C5C7 CRC64;
MTQQPPLSSI DLSGSWQLTS VDGEIRSVIT LPGDVHTALY RASLIPEPYF GRNEEKVQWV
AEREWAVERS FTLHDAEGDW YLDVDYLDTV ASIYVNGFLA LEADNSFRRY RPDVSSMLKS
GDNVIRIVFA SNPAVGAERQ KKQPFYIPYS TGNSPIPDGN MLRKPQCHFG WDWNIAIAPF
GLYGTVALKK LETARIEHVV TRQVHNNDGS VDLTVTASLF SKHAGVAQIY FEFDGERVRL
DVGVKGETDV NYLFHIDEPR LWWPSGSGEQ ALYTLSVELS DEVVTRQIGL RTIELITTPD
AAGARFAFRI NGREIFCRGA NWIPADALYS LSSPEKTEDL LQSAKAANMN MIRVWGGGFY
EQDHFYDLCD RLGLLVWQDF MFACNLYPSA EDFLDNVAIE VDYQVRRLSS HPSIALWCGD
NELVGALTWF EESRKDRDRY LVSYDRLNRT IEQAVKKALP GALWWPSSPA SGYLDFGDAW
HADGAGDMHY WSVWHENKSF DNYRTVRPRF CSEFGFQSYT SLPVIRTYAE EKDMNIASPV
MELHQKNAGG NERIAGTMFR YFRFPKDFPN FVYLSQIQQG LAIKTAVEYW RSLKPHCMGT
IYWQLNDTWP VASWSSLDYG GRWKAMHYLV KRFFQPVAVA AIPSEDGKTI RFSLVNDTLA
DVSVDLSISV LTMNGERKHL KDVQAVCSPD AAVTAATIDV AEIGEGTLLA WQFTASNGMG
GEGHYVNGTY KALELEPAGL TVTHEYVEAS GAVDINVTAK GLALFVMIES ETDGKYSDNA
FDLAAGESRR ITFTPAHQLE RGKLPDFRFY DLHSCQSAD
//
