ID Q3SE28_PARTE Unreviewed; 1596 AA.
AC Q3SE28;
DT 11-OCT-2005, integrated into UniProtKB/TrEMBL.
DT 11-OCT-2005, sequence version 1.
DT 27-MAR-2024, entry version 95.
DE SubName: Full=Chromosome undetermined scaffold_134, whole genome shotgun sequence {ECO:0000313|EMBL:CAK63262.1};
DE SubName: Full=Dicer-like ribonuclease with mutated helicase and Rnase III domains {ECO:0000313|EMBL:CAI39097.1};
GN Name=Dcr2 {ECO:0000313|EMBL:CAI39097.1};
GN ORFNames=GSPATT00033115001 {ECO:0000313|EMBL:CAK63262.1};
OS Paramecium tetraurelia.
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX NCBI_TaxID=5888 {ECO:0000313|EMBL:CAI39097.1};
RN [1] {ECO:0000313|EMBL:CAI39097.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=D4-2 {ECO:0000313|EMBL:CAI39097.1};
RA Genoscope;
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CAI39097.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=D4-2 {ECO:0000313|EMBL:CAI39097.1};
RA Serrano V., Meyer E.;
RT "RNA metabolism genes in Paramecium.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CAK63262.1, ECO:0000313|Proteomes:UP000000600}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Stock d4-2 {ECO:0000313|EMBL:CAK63262.1,
RC ECO:0000313|Proteomes:UP000000600};
RX PubMed=17086204; DOI=10.1038/nature05230;
RG Genoscope;
RA Aury J.-M., Jaillon O., Duret L., Noel B., Jubin C., Porcel B.M.,
RA Segurens B., Daubin V., Anthouard V., Aiach N., Arnaiz O., Billaut A.,
RA Beisson J., Blanc I., Bouhouche K., Camara F., Duharcourt S., Guigo R.,
RA Gogendeau D., Katinka M., Keller A.-M., Kissmehl R., Klotz C., Koll F.,
RA Le Moue A., Lepere C., Malinsky S., Nowacki M., Nowak J.K., Plattner H.,
RA Poulain J., Ruiz F., Serrano V., Zagulski M., Dessen P., Betermier M.,
RA Weissenbach J., Scarpelli C., Schachter V., Sperling L., Meyer E.,
RA Cohen J., Wincker P.;
RT "Global trends of whole-genome duplications revealed by the ciliate
RT Paramecium tetraurelia.";
RL Nature 444:171-178(2006).
RN [4] {ECO:0000313|EMBL:CAK63262.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Stock d4-2 {ECO:0000313|EMBL:CAK63262.1};
RG Genoscope;
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: Belongs to the helicase family. Dicer subfamily.
CC {ECO:0000256|PROSITE-ProRule:PRU00657}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CR932271; CAI39097.1; -; Genomic_DNA.
DR EMBL; CT868024; CAK63262.1; -; Genomic_DNA.
DR RefSeq; XP_001430660.1; XM_001430623.1.
DR STRING; 5888.Q3SE28; -.
DR EnsemblProtists; CAK63262; CAK63262; GSPATT00033115001.
DR GeneID; 5016444; -.
DR KEGG; ptm:GSPATT00033115001; -.
DR eggNOG; KOG0701; Eukaryota.
DR HOGENOM; CLU_245690_0_0_1; -.
DR InParanoid; Q3SE28; -.
DR OMA; IIQNQQY; -.
DR Proteomes; UP000000600; Partially assembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 3.30.160.380; Dicer dimerisation domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 1.10.1520.10; Ribonuclease III domain; 1.
DR InterPro; IPR038248; Dicer_dimer_sf.
DR InterPro; IPR005034; Dicer_dimerisation_dom.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR036389; RNase_III_sf.
DR PANTHER; PTHR14074:SF16; ANTIVIRAL INNATE IMMUNE RESPONSE RECEPTOR RIG-I; 1.
DR PANTHER; PTHR14074; HELICASE WITH DEATH DOMAIN-RELATED; 1.
DR Pfam; PF03368; Dicer_dimer; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF69065; RNase III domain-like; 1.
DR PROSITE; PS51327; DICER_DSRBF; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:CAI39097.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000000600};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00657}.
FT DOMAIN 118..315
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 561..705
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 744..840
FT /note="Dicer dsRNA-binding fold"
FT /evidence="ECO:0000259|PROSITE:PS51327"
FT COILED 560..587
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1596 AA; 187068 MW; F89CA26600A63D7D CRC64;
MYELPADDKK LENMDLQHNS FDVKLIEDDD EEDKSQISQF LQNVIKQPEE NLFDLDISES
QEQQNEPKDS LSSLVDQMLS SISNNQEILN NKVNLLEEDD TIVDTIEEFE FRDYQIELYE
KGIGKNSIIY LETGLGKTLV IIMLMWDRLF KYPDKKIVFL ANTVQLVEQQ AQQIKQKLPR
VAELISDDDS MIVKAKEIGS KLNVLHGSKC TDIWNQIMWQ IILEESKILV MTTQIFLNIL
RKGLVKISNF SFIAMDECHN AIQDHPYNYI LKEFYLKTKF QSDNQFLPQI VGATASPVMN
SKSVSNNQLL QELLQLSANM DSQYLHIDAQ NLKKHIKEAQ IVPIYYKMLF NDPDIQKVID
LKASLTNCEK NENKALDVMR RNSYLTEFFQ IKQTLENFKK KYSYDLQTKI LIAQIEKQVL
INGFQMYLEL GQYLFVLLVE YVIIKLNDFL TIKRPPNQAA CLDILKSIST ILKDAKLRYS
NVQQSSTPKV QVLFELIRKA YSQGDDNSRI LIFVKQRLTA FFLNRLIEEY LASERINIVS
NFIIGHCSSM VRKQNNSIMI NVIESNKEDQ EELIKKLYAE LQNDQSKFSI EQLKDQVNRL
NIFAYRISST VQNDIINKFR QGKIKILIST SVAEEGIDIP MCNYVIGFNP ISSSKAYVQM
KGRARKENSQ FMIFMVDKLQ QAQVQSHQQV QNNIKQIIEE LTAPQRQQML NKAMQTLQSK
TYENTYFDSF EIEGSGALIN TNWSCQLIQQ LCQKFNQKDA DKTQPKYAIY QLHGNAQTNQ
QFIAFLLLPI SMKSFIFYGK LAPSSKDAKA SAAFEASVQL YQKGYIDDNL QICFDSDLGQ
HIGADQDDSQ IMMTREQWQI ACKYSQRLVQ SQSTMGGKVN SQKRYYTNLF KTLFQTKDQE
GQFEDFLLYE CLFQNEKILL AFPKQMLPQI SKSENTLQAL GIQFQSMQKI SKSQYEGHYK
KYVETIIKEE NLQQWRDDIY AIINQKCQIR IKSESNSNLL LILKGSVKSK FTLSQIINQF
ALRFKVDLFQ DKIKEQLIQK IQIIPQNIKP HINEVLNSTS KYIKYLTSLQ YCYQNELDSL
QLNFIHYSVS DVVLNFDVKD RLDEILFKSL DLSSHKYNEL LLGHQYYKFL IAVVLYANYF
ENDINITRAK YKTFTRSTYV RNCLLESYLF LYLHKNDLND ITTQFIGLEK FQDNFDMMME
QLILDDFSVA KRKSQRNTIK NKIHISNQEF FQFIQNSLFL IQQNDQDLVN GLNWLRTFRI
MTSMKIHVNS SQCLQEFLPG FVPLSEQTRK IKRVFSSLEY KIKYQFKHFE LLFQAMTDIS
FKFVINNKLK FKYYEQFQKK EEFQGMIQEQ QWIEFKSQFE CMNETDQNNY ENSLLAMLGK
ALWNYLTIKI LNENNLDCFA IKIVGKILSK TPFLAYLAIQ LGLQQYLNSI YSTILLNLAS
EVEKCKDSSL AFKIGKIQKS IHTQCLETAF FALVGAIYLD SNCSSNIVMQ WATLILDNVQ
VQQFYSPDFI KRQPKYQFYQ WYQSNIGNPF NFNLIKIPHQ DYRRVSQGDK LFIPYDMLEA
QIVNIKKDKY YEISQLHIYA KSKEKAWEKL YEMIDY
//
