ID Q3SPC4_NITWN Unreviewed; 727 AA.
AC Q3SPC4;
DT 11-OCT-2005, integrated into UniProtKB/TrEMBL.
DT 11-OCT-2005, sequence version 1.
DT 27-MAR-2024, entry version 113.
DE RecName: Full=Anthranilate synthase {ECO:0000256|PIRNR:PIRNR036934};
DE EC=4.1.3.27 {ECO:0000256|PIRNR:PIRNR036934};
GN OrderedLocusNames=Nwi_2614 {ECO:0000313|EMBL:ABA05867.1};
OS Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 /
OS NCIMB 11846 / Nb-255).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Nitrobacter.
OX NCBI_TaxID=323098 {ECO:0000313|EMBL:ABA05867.1, ECO:0000313|Proteomes:UP000002531};
RN [1] {ECO:0000313|EMBL:ABA05867.1, ECO:0000313|Proteomes:UP000002531}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255
RC {ECO:0000313|Proteomes:UP000002531};
RX PubMed=16517654; DOI=10.1128/AEM.72.3.2050-2063.2006;
RA Starkenburg S.R., Chain P.S., Sayavedra-Soto L.A., Hauser L., Land M.L.,
RA Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J.,
RA Hickey W.J.;
RT "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium
RT Nitrobacter winogradskyi Nb-255.";
RL Appl. Environ. Microbiol. 72:2050-2063(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chorismate + L-glutamine = anthranilate + H(+) + L-glutamate +
CC pyruvate; Xref=Rhea:RHEA:21732, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16567, ChEBI:CHEBI:29748, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58359; EC=4.1.3.27;
CC Evidence={ECO:0000256|PIRNR:PIRNR036934};
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 1/5. {ECO:0000256|PIRNR:PIRNR036934}.
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DR EMBL; CP000115; ABA05867.1; -; Genomic_DNA.
DR RefSeq; WP_011315817.1; NC_007406.1.
DR AlphaFoldDB; Q3SPC4; -.
DR STRING; 323098.Nwi_2614; -.
DR MEROPS; C26.A09; -.
DR KEGG; nwi:Nwi_2614; -.
DR eggNOG; COG0147; Bacteria.
DR eggNOG; COG0512; Bacteria.
DR HOGENOM; CLU_006493_3_0_5; -.
DR OrthoDB; 9803598at2; -.
DR UniPathway; UPA00035; UER00040.
DR Proteomes; UP000002531; Chromosome.
DR GO; GO:0004049; F:anthranilate synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01743; GATase1_Anthranilate_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.60.120.10; Anthranilate synthase; 1.
DR InterPro; IPR005801; ADC_synthase.
DR InterPro; IPR019999; Anth_synth_I-like.
DR InterPro; IPR006805; Anth_synth_I_N.
DR InterPro; IPR015890; Chorismate_C.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR010112; TrpE-G_bact.
DR InterPro; IPR006221; TrpG/PapA_dom.
DR NCBIfam; TIGR01815; TrpE-clade3; 1.
DR NCBIfam; TIGR00566; trpG_papA; 1.
DR PANTHER; PTHR11236; AMINOBENZOATE/ANTHRANILATE SYNTHASE; 1.
DR PANTHER; PTHR11236:SF9; ANTHRANILATE SYNTHASE COMPONENT 1; 1.
DR Pfam; PF04715; Anth_synt_I_N; 1.
DR Pfam; PF00425; Chorismate_bind; 1.
DR Pfam; PF00117; GATase; 1.
DR PIRSF; PIRSF036934; TrpE-G; 1.
DR PRINTS; PR00097; ANTSNTHASEII.
DR PRINTS; PR00096; GATASE.
DR SUPFAM; SSF56322; ADC synthase; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 4: Predicted;
KW Amino-acid biosynthesis {ECO:0000256|PIRNR:PIRNR036934};
KW Aromatic amino acid biosynthesis {ECO:0000256|PIRNR:PIRNR036934};
KW Glutamine amidotransferase {ECO:0000256|PROSITE-ProRule:PRU00605};
KW Lyase {ECO:0000256|PIRNR:PIRNR036934, ECO:0000313|EMBL:ABA05867.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002531};
KW Tryptophan biosynthesis {ECO:0000256|PIRNR:PIRNR036934}.
FT DOMAIN 121..200
FT /note="Anthranilate synthase component I N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04715"
FT DOMAIN 246..498
FT /note="Chorismate-utilising enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00425"
FT DOMAIN 529..703
FT /note="Glutamine amidotransferase"
FT /evidence="ECO:0000259|Pfam:PF00117"
FT ACT_SITE 604
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 693
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 695
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 727 AA; 79687 MW; DAAF00B0CEFC2378 CRC64;
MNRTAFSLPA QSEYLTAGGL SISRSIEHFT GGSRLDDLIG LLDSRRGVVL SSGTTVPGRY
ESFDLGFADP PLKIETTGFN FTIEAANARG EVLIAFLGET LSEPCAVVTE KTARRIAGHI
VRGDAPVEED QRTRRASVMS LVRALVAAFA STADPMLGLY GAFAYDLVFQ IEDLVQKRAR
EPDQRDIVLF IPDRLLAYDR AAGHGVVLSY DFAWKGRSTE GQPRDTPDSV YAKTSRQGFT
DHAPGEYQAT VETARAAFAR GDLFEAVPGQ LFAEPCERSP AEVFQRLCRI NPSPYGGLVN
LGDGEFLVSA SPEMFVRSDG RRIETCPISG TIARGADAIG DAEQIRELLN SEKDEFELNM
CTDVDRNDKA RICMPGTIKV LARRQIETYS KLFHTVDHVE GILRSGFDAL DGFLTHAWAV
TVTGAPKKWA IQFVEDNERS TRRWYAGAFG VVGFDGSINT GLTIRTIRMK DGLAEVRVGA
TCLFDSKPEL EDRECQTKAA ALFQALRGDA PKPLSAVAPD ATGSGKRVLL IDHDDSFVHM
LADYFRQVGA RVSVVRYVHA RDMLDRQGYD LLVLSPGPGR PEDFGMARTI GLALEKKMPV
FGVCLGLQGI GEYFGGRLGQ LAQPAHGRPS RVQNRGGRLM RNLPNEIVIG RYHSLYVERD
RLPEVLEITA TTDDGVPMAI EHKSLPVGGV QFHPESLMSL GGEVGLRIVE NAFRLGRERR
ENSNDAV
//
