ID Q3USZ7_MOUSE Unreviewed; 541 AA.
AC Q3USZ7;
DT 11-OCT-2005, integrated into UniProtKB/TrEMBL.
DT 11-OCT-2005, sequence version 1.
DT 27-MAR-2024, entry version 139.
DE RecName: Full=Neuroepithelial cell-transforming gene 1 protein {ECO:0008006|Google:ProtNLM};
GN Name=Net1 {ECO:0000313|MGI:MGI:1927138};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|EMBL:BAE24183.1};
RN [1] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RX PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA Carninci P., Hayashizaki Y.;
RT "High-efficiency full-length cDNA cloning.";
RL Methods Enzymol. 303:19-44(1999).
RN [2] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RX PubMed=11042159; DOI=10.1101/gr.145100;
RA Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT full-length cDNA libraries for rapid discovery of new genes.";
RL Genome Res. 10:1617-1630(2000).
RN [3] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RX PubMed=11076861; DOI=10.1101/gr.152600;
RA Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT pipeline with 384 multicapillary sequencer.";
RL Genome Res. 10:1757-1771(2000).
RN [4] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RX PubMed=11217851; DOI=10.1038/35055500;
RG The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT "Functional annotation of a full-length mouse cDNA collection.";
RL Nature 409:685-690(2001).
RN [5] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RX PubMed=12466851; DOI=10.1038/nature01266;
RG The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT "Analysis of the mouse transcriptome based on functional annotation of
RT 60,770 full-length cDNAs.";
RL Nature 420:563-573(2002).
RN [6] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RA Arakawa T., Carninci P., Fukuda S., Hashizume W., Hayashida K., Hori F.,
RA Iida J., Imamura K., Imotani K., Itoh M., Kanagawa S., Kawai J., Kojima M.,
RA Konno H., Murata M., Nakamura M., Ninomiya N., Nishiyori H., Nomura K.,
RA Ohno M., Sakazume N., Sano H., Sasaki D., Shibata K., Shiraki T.,
RA Tagami M., Tagami Y., Waki K., Watahiki A., Muramatsu M., Hayashizaki Y.;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RG The FANTOM Consortium;
RG Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT "The Transcriptional Landscape of the Mammalian Genome.";
RL Science 309:1559-1563(2005).
RN [8] {ECO:0000313|EMBL:BAE24183.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE24183.1};
RC TISSUE=Egg {ECO:0000313|EMBL:BAE24183.1};
RX PubMed=16141073; DOI=10.1126/science.1112009;
RG RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT "Antisense Transcription in the Mammalian Transcriptome.";
RL Science 309:1564-1566(2005).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; AK139921; BAE24183.1; -; mRNA.
DR RefSeq; NP_001040624.1; NM_001047159.2.
DR RefSeq; NP_062645.2; NM_019671.3.
DR AlphaFoldDB; Q3USZ7; -.
DR SMR; Q3USZ7; -.
DR PeptideAtlas; Q3USZ7; -.
DR Antibodypedia; 10610; 479 antibodies from 36 providers.
DR DNASU; 56349; -.
DR GeneID; 56349; -.
DR KEGG; mmu:56349; -.
DR UCSC; uc007pjd.3; mouse.
DR AGR; MGI:1927138; -.
DR CTD; 10276; -.
DR MGI; MGI:1927138; Net1.
DR VEuPathDB; HostDB:ENSMUSG00000021215; -.
DR OrthoDB; 24012at2759; -.
DR BioGRID-ORCS; 56349; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Net1; mouse.
DR ExpressionAtlas; Q3USZ7; baseline and differential.
DR Genevisible; Q3USZ7; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR CDD; cd13224; PH_Net1; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR001331; GDS_CDC24_CS.
DR InterPro; IPR037853; Net1_PH.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR46006:SF4; NEUROEPITHELIAL CELL-TRANSFORMING GENE 1 PROTEIN; 1.
DR PANTHER; PTHR46006; RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR PROSITE; PS00741; DH_1; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
FT DOMAIN 120..302
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 332..447
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT REGION 70..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 501..541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 521..541
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 541 AA; 61765 MW; 38993EDD3F34D2DD CRC64;
MVAHDEIGGL LPIKRTIRVL DVNNQPFREQ EEPSNKRVRP LARVTSLANL ISPVRNGAVR
RFGQTIQSFT LRGDHRSPAS AQKSFSRSTV PTPTKRRSSA LWSEMLDINM KESLTTREIK
RQEAIYELSR GEQDLIEDLK LARKAYHDPM LKLSIMSEEE LTHIFGDLDA YIPLHEDLLA
RIGEATKPDG TVEQIGHILV NWLPGLNAYR GYCSNQLAAK ALLDQKKQDP RVQDFLQRCL
ESPFSRKLDL WSFLDIPRSR LVKYPLLLKE ILRHTPKDHR DVQLLEEAIL IIQGVLSDIN
LKKGESECQY YINKLEYLDE KQKDPRIEAS KVLLCHGELK NKSGHKLYIF LFQDILVLTR
PVTRNERHLY QVYRQPIPVQ ELVLEDLQDG DVRMGGSFRG AFGNSDKAKN IFRVRFQDPS
PGHSHTLQAN DVFHKQQWFN CIRAAIAPFQ RAASPLELQG LPDLHEECEE NNPSAGNLRA
QRRSCVVPGV MQIDEESALD CGSSVQTVED TRNMKAQRPQ PGLRRARDKA QSGGKKKETL
V
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