ID   Q45VN5_ICTPU            Unreviewed;        84 AA.
AC   Q45VN5;
DT   13-SEP-2005, integrated into UniProtKB/TrEMBL.
DT   13-SEP-2005, sequence version 1.
DT   27-MAR-2024, entry version 58.
DE   RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|RuleBase:RU369009};
DE            EC=2.3.2.26 {ECO:0000256|RuleBase:RU369009};
DE   Flags: Fragment;
OS   Ictalurus punctatus (Channel catfish) (Silurus punctatus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC   Ictaluridae; Ictalurus.
OX   NCBI_TaxID=7998 {ECO:0000313|EMBL:AAZ66790.1};
RN   [1] {ECO:0000313|EMBL:AAZ66790.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC CRL-2772 {ECO:0000313|EMBL:AAZ66790.1};
RC   TISSUE=Ovary {ECO:0000313|EMBL:AAZ66790.1};
RA   Yeh H.-Y., Klesius P.H.;
RT   "Differential gene expression of Ictalurus punctatus after Edwardsiella
RT   ictaluri.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an
CC       E2 ubiquitin-conjugating enzyme in the form of a thioester and then
CC       directly transfers the ubiquitin to targeted substrates.
CC       {ECO:0000256|RuleBase:RU369009}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26; Evidence={ECO:0000256|RuleBase:RU369009};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|RuleBase:RU369009}.
CC   -!- SIMILARITY: Belongs to the UPL family. K-HECT subfamily.
CC       {ECO:0000256|RuleBase:RU369009}.
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DR   EMBL; DQ103698; AAZ66790.1; -; mRNA.
DR   AlphaFoldDB; Q45VN5; -.
DR   UniPathway; UPA00143; -.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   InterPro; IPR045322; HECTD1/TRIP12-like.
DR   PANTHER; PTHR45670:SF19; E3 UBIQUITIN-PROTEIN LIGASE HECTD1; 1.
DR   PANTHER; PTHR45670; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   PROSITE; PS50237; HECT; 1.
PE   2: Evidence at transcript level;
KW   Transferase {ECO:0000256|RuleBase:RU369009, ECO:0000313|EMBL:AAZ66790.1};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   DOMAIN          15..84
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   ACT_SITE        53
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:AAZ66790.1"
SQ   SEQUENCE   84 AA;  9430 MW;  9432B6057132071D CRC64;
     TVWNVLTREE SLPPFTTGCS TLPPGGLANL HPRLTIVRKV DATDASYPSV NTCVHYLKLP
     EYSSEEIMRE RLLDATMEKG FHLN
//