ID Q48614_9LACT Unreviewed; 599 AA.
AC Q48614;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2001, sequence version 2.
DT 27-MAR-2024, entry version 71.
DE SubName: Full=AbiK {ECO:0000313|EMBL:AAB53491.2};
GN Name=abiK {ECO:0000313|EMBL:AAB53491.2};
OS Lactococcus lactis.
OG Plasmid pSRQ800 {ECO:0000313|EMBL:AAB53491.2}.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=1358 {ECO:0000313|EMBL:AAB53491.2};
RN [1] {ECO:0000313|EMBL:AAB53491.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=W-1 {ECO:0000313|EMBL:AAB53491.2};
RC PLASMID=pSRQ800 {ECO:0000313|EMBL:AAB53491.2};
RX PubMed=9097424;
RA Emond E., Holler B.J., Boucher I., Vandenbergh P.A., Vedamuthu E.R.,
RA Kondo J.K., Moineau S.;
RT "Phenotypic and genetic characterization of the bacteriophage abortive
RT infection mechanism AbiK from Lactococcus lactis.";
RL Appl. Environ. Microbiol. 63:1274-1283(1997).
RN [2] {ECO:0000313|EMBL:AAB53491.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=W-1 {ECO:0000313|EMBL:AAB53491.2};
RC PLASMID=pSRQ800 {ECO:0000313|EMBL:AAB53491.2};
RX PubMed=10708383;
RA Boucher I., Emond E., Dion E., Montpetit D., Moineau S.;
RT "Microbiological and molecular impacts of AbiK on the lytic cycle of
RT Lactococcus lactis phages of the 936 and P335 species.";
RL Microbiology 146:445-453(2000).
RN [3] {ECO:0000313|EMBL:AAB53491.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=W-1 {ECO:0000313|EMBL:AAB53491.2};
RC PLASMID=pSRQ800 {ECO:0000313|EMBL:AAB53491.2};
RX PubMed=11467810;
RA Boucher I., Emond E., Parrot M., Moineau S.;
RT "DNA sequence analysis of three Lactococcus lactis plasmids encoding phage
RT resistance mechanisms.";
RL J. Dairy Sci. 84:1610-1620(2001).
RN [4] {ECO:0007829|PDB:7R06, ECO:0007829|PDB:7R07}
RP STRUCTURE BY ELECTRON MICROSCOPY (2.27 ANGSTROMS) IN COMPLEX WITH MG(2+).
RX PubMed=36107766; DOI=10.1093/nar/gkac772;
RA Figiel M., Gapinska M., Czarnocki-Cieciura M., Zajko W., Sroka M.,
RA Skowronek K., Nowotny M.;
RT "Mechanism of protein-primed template-independent DNA synthesis by Abi
RT polymerases. .";
RL Nucleic Acids Res. 50:10026-10040(2022).
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DR EMBL; U35629; AAB53491.2; -; Genomic_DNA.
DR RefSeq; NP_862563.1; NC_004960.1.
DR RefSeq; WP_011117213.1; NC_004960.1.
DR PDB; 7R06; EM; 2.27 A; A/B/C/D/E/F=1-599.
DR PDB; 7R07; X-ray; 3.10 A; A/B/C/D/E/F=1-599.
DR PDB; 7Z0Z; EM; 2.68 A; A/B/C/D/E/F=1-599.
DR AlphaFoldDB; Q48614; -.
DR EMDB; EMD-14220; -.
DR EMDB; EMD-14435; -.
DR SMR; Q48614; -.
DR CDD; cd01646; RT_Bac_retron_I; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR000477; RT_dom.
DR Pfam; PF00078; RVT_1; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:7R06, ECO:0007829|PDB:7R07};
KW Metal-binding {ECO:0007829|PDB:7R07};
KW Plasmid {ECO:0000313|EMBL:AAB53491.2}.
FT DOMAIN 1..326
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
FT BINDING 163
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:7R07"
FT BINDING 163
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:7R07"
FT BINDING 164
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:7R07"
FT BINDING 247
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:7R07"
FT BINDING 247
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:7R07"
FT BINDING 248
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:7R07"
SQ SEQUENCE 599 AA; 71401 MW; C54BDA32A61BE634 CRC64;
MKKEFTELYD FIFDPIFLVR YGYYDRSIKN KKMNTAKVEL DNEYGKSDSF YFKVFNMESF
ADYLRSHDLK THFNGKKPLS TDPVYFNIPK NIEARRQYKM PNLYSYMALN YYICDNKKEF
IEVFIDNKFS TSKFFNQLNF DYPKTQEITQ TLLYGGIKKL HLDLSNFYHT LYTHSIPWMI
DGKSASKQNR KKGFSNTLDT LITACQYDET HGIPTGNLLS RIITELYMCH FDKQMEYKKF
VYSRYVDDFI FPFTFENEKQ EFLNEFNLIC RENNLIINDN KTKVDNFPFV DKSSKSDIFS
FFENITSTNS NDKWIKEISN FIDYCVNEEH LGNKGAIKCI FPVITNTLKQ KKVDTKNIDN
IFSKRNMVTN FNVFEKILDL SLKDSRLTNK FLTFFENINE FGFSSLSASN IVKKYFSNNS
KGLKEKIDHY RKNNFNQELY QILLYMVVFE IDDLLNQEEL LNLIDLNIDD YSLILGTILY
LKNSSYKLEK LLKKIDQLFI NTHANYDVKT SRMAEKLWLF RYFFYFLNCK NIFSQKEINS
YCQSQNYNSG QNGYQTELNW NYIKGQGKDL RANNFFNELI VKEVWLISCG ENEDFKYLN
//
