ID Q48857_9LACO Unreviewed; 566 AA.
AC Q48857;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 92.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
OS Lactobacillus sp.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=1591 {ECO:0000313|EMBL:BAA06144.1};
RN [1] {ECO:0000313|EMBL:BAA06144.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=No.1 {ECO:0000313|EMBL:BAA06144.1};
RA Maeda T., Kawano S., Takagi M., Imanaka T.;
RT "Nucleotide sequence of the neutral protease gene (nprL) from Lactobacillus
RT sp. and characterization of the enzyme.";
RL J. Ferment. Bioeng. 0:0-0(1994).
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947,
CC ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
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DR EMBL; D29673; BAA06144.1; -; Genomic_DNA.
DR AlphaFoldDB; Q48857; -.
DR MEROPS; M04.018; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.40; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR025711; PepSY.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF3; NEUTRAL PROTEASE B; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF03413; PepSY; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 28..566
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023157250"
FT DOMAIN 84..134
FT /note="FTP"
FT /evidence="ECO:0000259|Pfam:PF07504"
FT DOMAIN 154..219
FT /note="PepSY"
FT /evidence="ECO:0000259|Pfam:PF03413"
FT DOMAIN 257..400
FT /note="Peptidase M4"
FT /evidence="ECO:0000259|Pfam:PF01447"
FT DOMAIN 403..565
FT /note="Peptidase M4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02868"
FT REGION 229..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 393
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 481
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 566 AA; 60964 MW; 9CCA8FF298C9A04E CRC64;
MKKKSLALVL ATGMAVTTFG GTGSAFADSK NVLSTKKYNE TVQSPEFVSG DLTEATGKKA
ESVVFDYLNA AKGDYKLGEK SAQDSFKVKQ AKKDAVTDST VLRLQQVYEG VPVWGSTQVA
HVSKDGSLKV LSGTVAPDLD KKEKLKNKNK IEGAKAIEIA QKDLGVTPKY EVEPKADLYV
YQNGEETTYA YVVNLNFLEP SPGNYYYFIE ADSGKVLNKY NKLDHVANED KSPVKQEAPK
QEAKPAVKPV TGTNAVGTGK GVLGDTKSLN TTLSASSYYL QDNTRGATIF TYDAKNRSTL
PGTLWVDADN VFNAAYDAAA VDAHYYAGKT YDYYKATFNR NSINDAGAPL KSTVHYGSKY
NNAFWNGSQM VYGDGDGVTF TSLSGGIDVI GHELTHAVTE YSSDLIYQNE SGALNEAISD
VFGTLVEYYD NRNPDWEIGE DIYTPGKAGD ALRSMSDPTK YGDPDHYSKR YTGTSDNGGV
HTNSGIINKA AYLLANGGTH YGVTVNGIGK DKVGAIYYRA NTQYFTQSTT FSQARAGLVQ
AAADLYGASS AEVAAVKQSY SAVGVN
//
