ID Q52068_ENTAG Unreviewed; 275 AA.
AC Q52068;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=Nitrogen fixation protein NifU {ECO:0000256|ARBA:ARBA00015278, ECO:0000256|PIRNR:PIRNR000375};
GN Name=nifU {ECO:0000313|EMBL:CAA68019.1};
OS Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans).
OG Plasmid pEA3 {ECO:0000313|EMBL:CAA68019.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX NCBI_TaxID=549 {ECO:0000313|EMBL:CAA68019.1};
RN [1] {ECO:0000313|EMBL:CAA68019.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pEA3 {ECO:0000313|EMBL:CAA68019.1};
RA Kreutzer R., Steibl H.D., Dayananda S., Dippe R., Halda L., Buck M.,
RA Klingmueller W.;
RT "Genetic characterization of nitrogen fixation in Enterobacter strains from
RT the rhizosphere of cereals.";
RL (In) Polsinelli M., Materassi R., Vincenzini M. (eds.);
RL NITROGEN FIXATION, pp.24-26, Kluwer Academic Publishers, Dortrecht, Boston,
RL London (1991).
RN [2] {ECO:0000313|EMBL:CAA68019.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pEA3 {ECO:0000313|EMBL:CAA68019.1};
RA Deuerling E.;
RL Thesis (1992), Diplomarbeit, Universitaet Bayreuth, Bayreuth, Germany.
RN [3] {ECO:0000313|EMBL:CAA68019.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pEA3 {ECO:0000313|EMBL:CAA68019.1};
RA Steibl R., Steibl H.D., Siddavattam D., Klingmeuller W.;
RT "Identification of a new nif-gene, nifI in the nifUSVWZM-operon of
RT Enterobacter agglomerans 333.";
RL (In) Palacios R., Mora J., Newton W.E. (eds.);
RL NEW HORIZONS IN NITROGEN FIXATION, pp.496-496, Kluwer Academic Publishers,
RL Dortrecht (1993).
RN [4] {ECO:0000313|EMBL:CAA68019.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pEA3 {ECO:0000313|EMBL:CAA68019.1};
RA Lechler A.;
RL Thesis (1993), Diplomarbeit, Universitaet Bayreuth, Bayreuth, Germany.
RN [5] {ECO:0000313|EMBL:CAA68019.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pEA3 {ECO:0000313|EMBL:CAA68019.1};
RA Schwickerath O.;
RL Thesis (1994), Diplomarbeit, Universitaet Bayreuth, Bayreuth, Germany.
RN [6] {ECO:0000313|EMBL:CAA68019.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pEA3 {ECO:0000313|EMBL:CAA68019.1};
RA Steibl H.;
RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:CAA68019.1}
RP NUCLEOTIDE SEQUENCE.
RC PLASMID=pEA3 {ECO:0000313|EMBL:CAA68019.1};
RA Lechler A., Deuerling E., Steibl H.D., Klingmeuller W.;
RT "The nifENX-operon of Enterobacter agglomerans 333.";
RL Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in the formation or repair of [Fe-S] clusters
CC present in iron-sulfur proteins. {ECO:0000256|PIRNR:PIRNR000375}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000256|ARBA:ARBA00006420,
CC ECO:0000256|PIRNR:PIRNR000375}.
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DR EMBL; X99694; CAA68019.1; -; Genomic_DNA.
DR AlphaFoldDB; Q52068; -.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd06664; IscU_like; 1.
DR CDD; cd19947; NifU_Fer2_BFD-like; 1.
DR Gene3D; 3.90.1010.10; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.30.300.130; Fe-S cluster assembly (FSCA); 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR016217; N_fixation_NifU.
DR InterPro; IPR010238; NIF_FeS_clus_asmbl_NifU.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR NCBIfam; TIGR02000; NifU_proper; 1.
DR PANTHER; PTHR10093:SF8; IRON-SULFUR CLUSTER ASSEMBLY ENZYME ISCU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR10093; IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF01106; NifU; 1.
DR Pfam; PF01592; NifU_N; 1.
DR PIRSF; PIRSF000375; NifU; 1.
DR SUPFAM; SSF117916; Fe-S cluster assembly (FSCA) domain-like; 1.
DR SUPFAM; SSF82649; SufE/NifU; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR000375-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000375-1};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|PIRNR:PIRNR000375}; Plasmid {ECO:0000313|EMBL:CAA68019.1}.
FT DOMAIN 4..123
FT /note="NIF system FeS cluster assembly NifU N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01592"
FT DOMAIN 135..184
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 208..272
FT /note="NIF system FeS cluster assembly NifU C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01106"
FT BINDING 35
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 62
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 106
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 137
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 139
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 172
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 175
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
SQ SEQUENCE 275 AA; 29956 MW; 83D2A4D9E888A73A CRC64;
MWNYSEKVKE HFFNPRNAKV VDDANAIGDV GSLSCGDALR LMLKVDPETE IILDAGFQTF
GCGSAIASSS ALTELIIGRS LTQAQQVSNQ QIADYLDGLP PEKMHCSVMG QEALRAAINN
FRGEAPDEDH EEGALICKCF AVDEAQIRRA VLANGLTTLE DVINYTKAGG GCTSCHEKIE
LCLEEILKEH PVKNPVAAKV RDENWLAVEE VVASLRPHIR ADGGDMSLVR VDGQEVTVEL
SGSCSGCMMT DMTLAWLQQK LVERMGRYIR VVSAA
//
