ID Q52293_PSEPU Unreviewed; 400 AA.
AC Q52293;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1996, sequence version 1.
DT 24-JAN-2024, entry version 79.
DE RecName: Full=General secretion pathway protein F {ECO:0000256|ARBA:ARBA00030750};
GN Name=xcpS {ECO:0000313|EMBL:CAA56981.1};
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303 {ECO:0000313|EMBL:CAA56981.1};
RN [1] {ECO:0000313|EMBL:CAA56981.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WCS358 {ECO:0000313|EMBL:CAA56981.1};
RX PubMed=8602167; DOI=10.1007/s004380050102;
RA de Groot A., Krijger J.J., Filloux A., Tommassen J.;
RT "Characterization of type II protein secretion (xcp) genes in the plant
RT growth-stimulating Pseudomonas putida, strain WCS358.";
RL Mol. Gen. Genet. 250:491-504(1996).
RN [2] {ECO:0000313|EMBL:CAA56981.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=WCS358 {ECO:0000313|EMBL:CAA56981.1};
RA de Groot A.;
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the type II secretion system inner membrane
CC complex required for the energy-dependent secretion of extracellular
CC factors such as proteases and toxins from the periplasm.
CC {ECO:0000256|ARBA:ARBA00002684}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429, ECO:0000256|RuleBase:RU003923}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004429,
CC ECO:0000256|RuleBase:RU003923}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the GSP F family.
CC {ECO:0000256|ARBA:ARBA00005745, ECO:0000256|RuleBase:RU003923}.
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DR EMBL; X81085; CAA56981.1; -; Genomic_DNA.
DR PIR; S64729; S64729.
DR AlphaFoldDB; Q52293; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015627; C:type II protein secretion system complex; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015628; P:protein secretion by the type II secretion system; IEA:InterPro.
DR Gene3D; 1.20.81.30; Type II secretion system (T2SS), domain F; 2.
DR InterPro; IPR003004; GspF/PilC.
DR InterPro; IPR011850; T2SS_GspF.
DR InterPro; IPR001992; T2SS_GspF/T4SS_PilC_CS.
DR InterPro; IPR018076; T2SS_GspF_dom.
DR InterPro; IPR042094; T2SS_GspF_sf.
DR NCBIfam; TIGR02120; GspF; 1.
DR PANTHER; PTHR30012; GENERAL SECRETION PATHWAY PROTEIN; 1.
DR PANTHER; PTHR30012:SF0; TYPE II SECRETION SYSTEM PROTEIN F-RELATED; 1.
DR Pfam; PF00482; T2SSF; 2.
DR PRINTS; PR00812; BCTERIALGSPF.
DR PROSITE; PS00874; T2SP_F; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU003923};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU003923}.
FT TRANSMEM 165..187
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 218..237
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 371..392
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 66..188
FT /note="Type II secretion system protein GspF"
FT /evidence="ECO:0000259|Pfam:PF00482"
FT DOMAIN 269..390
FT /note="Type II secretion system protein GspF"
FT /evidence="ECO:0000259|Pfam:PF00482"
SQ SEQUENCE 400 AA; 43644 MW; 4EF4E58248DC6B92 CRC64;
MPTYRYQAVD LAGKTHKASL QADSERHARQ LLRDQGLFAR QLQRHEAGVR QPRHQRLSRA
QLCELTRQLA TLTGAGIPLV DALATLERQL RQPALHSVLV ALRGSLAEGL GLARSLARQG
ASFSGLYCAL VEAGERSGRL AQVLTRLADH LEQVQRQQHK ARTALIYPCV LMGVSLAVVI
GLMTFVVPKL TEQFAHAGQS LPLITALLIG LSQGLVHAGP WLLGLAMLLT LLGGWLLRKP
HWCLRRDELL LRLPRVGGLL QVLESARLAR SLAILSSSGV ALLEALQVAT ETVSNRRIRL
AMEQVRQQVQ GGTSLHRALD ACQQFPPLLV NMVGSGEASG TLADMLERVA DDQERGFARQ
VDTAMALFEP LMILVMGAVV LFIVLAVLLP IMQLNQGLQL
//
