ID Q54LM5_DICDI Unreviewed; 615 AA.
AC Q54LM5;
DT 24-MAY-2005, integrated into UniProtKB/TrEMBL.
DT 24-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 102.
DE RecName: Full=Pseudouridine synthase I TruA alpha/beta domain-containing protein {ECO:0000259|Pfam:PF01416};
GN ORFNames=DDB_G0286567 {ECO:0000313|EMBL:EAL64039.1};
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689 {ECO:0000313|EMBL:EAL64039.1, ECO:0000313|Proteomes:UP000002195};
RN [1] {ECO:0000313|EMBL:EAL64039.1, ECO:0000313|Proteomes:UP000002195}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4 {ECO:0000313|EMBL:EAL64039.1,
RC ECO:0000313|Proteomes:UP000002195};
RX PubMed=15875012; DOI=10.1038/nature03481;
RG The Dictyostelium discoideum Sequencing Consortium;
RA Eichinger L., Pachebat J.A., Glockner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M., Spiegler S., Tivey A., Sugano S.,
RA White B., Walker D., Woodward J., Winckler T., Tanaka Y., Shaulsky G.,
RA Schleicher M., Weinstock G., Rosenthal A., Cox E.C., Chisholm R.L.,
RA Gibbs R., Loomis W.F., Platzer M., Kay R.R., Williams J., Dear P.H.,
RA Noegel A.A., Barrell B., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAL64039.1}.
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DR EMBL; AAFI02000089; EAL64039.1; -; Genomic_DNA.
DR RefSeq; XP_637536.1; XM_632444.1.
DR AlphaFoldDB; Q54LM5; -.
DR SMR; Q54LM5; -.
DR STRING; 44689.Q54LM5; -.
DR PaxDb; 44689-DDB0187011; -.
DR GeneID; 8625675; -.
DR KEGG; ddi:DDB_G0286567; -.
DR dictyBase; DDB_G0286567; -.
DR eggNOG; KOG2553; Eukaryota.
DR HOGENOM; CLU_444414_0_0_1; -.
DR InParanoid; Q54LM5; -.
DR OMA; MISCESQ; -.
DR PhylomeDB; Q54LM5; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142:SF27; PSEUDOU_SYNTH_1 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 2.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000002195};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 393..478
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 92..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 328..359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 558..615
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..119
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..355
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 559..615
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 209
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 277
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 615 AA; 72613 MW; 9CE78F56B0F3E5EA CRC64;
MFRIIKNVNK LLINNNNSYY NNNSRNLQIK NYFASSKKEI GEIIEKRMDR GKIKERKKTK
FVSQAYRSII ERREEEVVII DKIRMDTGEI KESQTNYTSV PERGETEDIA NDEKKSNSPK
SLHITDLIEF CNTHNQEIEQ NLKDKSKKPM ALCFAYNGSK YYGSSYSFDI RFPAIENLIE
KSLIRNGHIA LENIGNLKRI KLSRSSRTDK GVHSVSTVIS AFLLIDKETE RLAQFGTPPQ
YFIDNINRYL PSDIRLISAT KPSRTFRARP ASKSRVYNYL VPMDHLKDIS IDRMNSILSN
FIGTSSFHNY TYQRFTYLDS NTLKKLSKIN DTQDENENND DEEDEEEDDE EDGNDNTKID
IEKEVEELEK LENKKNIEID RLAESELFRK NPVNVRTIMN FYVEKETVLF EGKQWYRFVV
VGDSFLMYQI RKMMGFFLSV VKGYQNEIGL QLSLKSPFSI CSPISPPFPL FLYDVNFPRE
KILLPVSRQI QNEFLNSKLM PHLHKIENET NQFSEFFNQL STFNFRLDDI DQLIILNKEF
HEKIELKKAE REKRIEDYNN NYNNNNNYNN NNNNNNNNNN NNNNNNNNNN NKNNNYNYNN
NYNNIYNNYN NYKRK
//
