ID Q57VM9_TRYB2 Unreviewed; 1034 AA.
AC Q57VM9; D6XMV4;
DT 10-MAY-2005, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 96.
DE RecName: Full=Polyribonucleotide 5'-hydroxyl-kinase Clp1 P-loop domain-containing protein {ECO:0000259|Pfam:PF16575};
GN Name=Tb08.29O4.440 {ECO:0000313|EMBL:AAZ12896.1};
GN ORFNames=Tb927.8.1260 {ECO:0000313|EMBL:AAX70329.1};
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431 {ECO:0000313|EMBL:AAX70329.1, ECO:0000313|Proteomes:UP000008524};
RN [1] {ECO:0000313|EMBL:AAZ12896.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ12896.1};
RX PubMed=16020724; DOI=10.1126/science.1112181;
RA El-Sayed N.M., Myler P.J., Blandin G., Berriman M., Crabtree J.,
RA Aggarwal G., Caler E., Renauld H., Worthey E.A., Hertz-Fowler C.,
RA Ghedin E., Peacock C., Bartholomeu D.C., Haas B.J., Tran A.N.,
RA Wortman J.R., Alsmark U.C., Angiuoli S., Anupama A., Badger J.,
RA Bringaud F., Cadag E., Carlton J.M., Cerqueira G.C., Creasy T.,
RA Delcher A.L., Djikeng A., Embley T.M., Hauser C., Ivens A.C.,
RA Kummerfeld S.K., Pereira-Leal J.B., Nilsson D., Peterson J., Salzberg S.L.,
RA Shallom J., Silva J.C., Sundaram J., Westenberger S., White O.,
RA Melville S.E., Donelson J.E., Andersson B., Stuart K.D., Hall N.;
RT "Comparative genomics of trypanosomatid parasitic protozoa.";
RL Science 309:404-409(2005).
RN [2] {ECO:0000313|EMBL:AAZ12896.1, ECO:0000313|Proteomes:UP000008524}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ12896.1,
RC ECO:0000313|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [3] {ECO:0000313|EMBL:AAX70329.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GUTat10.1 {ECO:0000313|EMBL:AAX70329.1};
RA Ghedin E., Blandin G., Bartholomeu D., Caler E., Haas B., Hannick L.,
RA Shallom J., Hou L., Djikeng A., Feldblyum T., Hostetler J., Johnson J.,
RA Jones K., Koo H.L., Larkin C., Pai G., Peterson J., Khalak H.G.,
RA Salzberg S., Simpson A.J., Tallon L., Van Aken S., Wanless D., White O.,
RA Wortman J., Fraser C.M., El-Sayed N.M.A.;
RT ".";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AAZ12896.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=927/4 GUTat10.1 {ECO:0000313|EMBL:AAZ12896.1};
RA Ghedin E., Blandin G., Bartholomeu D., Caler E., Haas B., Hannick L.,
RA Shallom J., Hou L., Djikeng A., Feldblyum T., Hostetler J., Johnson J.,
RA Jones K., Koo H.L., Larkin C., Pai G., Peterson J., Khalak H.G.,
RA Salzberg S., Simpson A.J., Tallon L., Van Aken S., Wanless D., White O.,
RA Wortman J., Fraser C.M., El-Sayed N.M.A.;
RT "Sequencing, closure, and annotation of Trypanosoma brucei chromosomes 2
RT through 8.";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the Clp1 family. NOL9/GRC3 subfamily.
CC {ECO:0000256|ARBA:ARBA00011003}.
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DR EMBL; AC159438; AAX70329.1; -; Genomic_DNA.
DR EMBL; CP000071; AAZ12896.1; -; Genomic_DNA.
DR RefSeq; XP_846962.1; XM_841869.1.
DR AlphaFoldDB; Q57VM9; -.
DR STRING; 185431.Q57VM9; -.
DR PaxDb; 5691-AAZ12896; -.
DR GeneID; 3659102; -.
DR KEGG; tbr:Tb927.8.1260; -.
DR VEuPathDB; TriTrypDB:Tb927.8.1260; -.
DR eggNOG; KOG2750; Eukaryota.
DR InParanoid; Q57VM9; -.
DR OMA; THICIIS; -.
DR OrthoDB; 5480745at2759; -.
DR Proteomes; UP000008524; Chromosome 8.
DR GO; GO:0005730; C:nucleolus; IDA:GeneDB.
DR GO; GO:0005634; C:nucleus; IDA:GeneDB.
DR GO; GO:0005819; C:spindle; IDA:GeneDB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0051731; F:polynucleotide 5'-hydroxyl-kinase activity; IBA:GO_Central.
DR GO; GO:0000448; P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR045116; Clp1/Grc3.
DR InterPro; IPR032319; CLP1_P.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12755; CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P; 1.
DR PANTHER; PTHR12755:SF3; POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9; 1.
DR Pfam; PF16575; CLP1_P; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000008524};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 596..733
FT /note="Polyribonucleotide 5'-hydroxyl-kinase Clp1 P-loop"
FT /evidence="ECO:0000259|Pfam:PF16575"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 50..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 188..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 234..271
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 524..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..71
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..209
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 245..267
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1034 AA; 112017 MW; 192D4FF62C49C066 CRC64;
MRRRRQPLTA GITEVDVGEG EEKKARDGTD FPAVNICSWS VSADAIAADL GNQHEGKGDR
RDVGDEADVG PPLKRFKIPD KALARIEAQA ALARDNVAEQ QNPAVGDDAT DVIRRRKFSA
LKYCTEVADE RDFSGVKKPL ISVGRLPRHT AEQRQQLRRA QRNVKNMTST SGPLGLFADG
GDVIRFGSVG SDSSDSNPSE TSSFDDEGCR TLTGNAVGRS ASAAREIRNI DRHFSGYDSE
DLPPVSGLLD EETEESEEYE ESEESEEERK IADSGVNFGD CCAEANGGTV KVMSVKRGFG
FQQQRRLQEG ARSPGNQHIA DEGTEIGVGN ETAAGAAVST GRCQRYGGVL FEMHINRAAA
APCHHTLLHV RNSITVQGPC CIIGFGIGEI TVGGFFLGRK HITLLSDSQK VVVMPVRPLK
SKSAASESLP VPKRQLHLPA ADDCVVVAPP ASASFCRFMG IGEPTAAGDT ASERDCEADD
LEMFGTVDWD WVEASVARVQ EGRHAADNSC VVLVVQPYKS HEKAPTRRRY LVPRDTSSSK
RENGGGRHRG KLHSDSFVEV PLFLARQPVP GVDAALLPDV VPAVVRHGSG AVVVLGSKGI
GKSTLCRFLA NALLSQHGVC YWLELDIGQP EFGPPGVLSL YCVSKPLLAP HESDGVDFVR
GYYVGGTRLR CPVAGATALT RICGVAEQLR KRYPIIVNTH GWVLSTGRRM TVEAVRRLQP
THIIHLAKRQ EEQWARFATE LVDPIKGLNA SVVNKRFLVH KQDGIACGAA GADSLPSSRI
LGLLPHAHDF RPKRSSTSAE ATKSSSLPKW SGTVHTVQVV RDEGLLRKSM QPKASAIRRG
MWGRYLAPIF EYYEATRLRH VKQQVSELYK GEEPIIVGGR RTTTLFTGEL SHFESLVFTD
VADASDLTVD FVCAALAHSV VAFSIRVISS SPAQGVAVSH KTESLTNCDA QAFHYPNCRS
LVDLPDGFPI TCFGIVESNE ADMRSSGKIR IRLPVGRDDV HKMLSVSGEG KRVCISLACT
LADRSETAAV EALF
//
